FastQCFastQC Report
Thu 26 May 2016
SRR1546785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546785_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1972181
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159840.8104732780611922No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99220.5030978393970938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG207.8578544E-444.012
TCGACGA600.044.02
TCACGAC4950.043.55555725
CGTTTTT220050.043.1901861
CGACGGT5050.043.12871628
CACGACG5150.041.8640826
CGGTCTA5150.041.8640831
GAGCGAT13350.041.5280887
GACGGTC5350.040.7102829
ACGGTCT5350.040.7102830
TCTCACG5300.040.6792523
GTTTTTA99400.039.5955732
TTTCGCG404.1265412E-738.51
CTACGCG404.1265412E-738.51
AATCGCG404.1265412E-738.51
TCGCGAG1150.038.260873
CGCGAGT357.2928615E-637.7142874
TACGCGA700.037.7142872
GAGACCG3850.037.7142877
TCGCGGA357.2928615E-637.7142872
Produced by FastQC (version 0.11.5)