##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546785_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1972181
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.456269987389597	31.0	31.0	33.0	30.0	34.0
2	31.844597934976555	31.0	31.0	34.0	30.0	34.0
3	31.919047491077137	31.0	31.0	34.0	30.0	34.0
4	35.659505897278194	37.0	35.0	37.0	33.0	37.0
5	35.55123439481468	37.0	35.0	37.0	33.0	37.0
6	35.59959658875123	37.0	35.0	37.0	33.0	37.0
7	36.01156993196872	37.0	35.0	37.0	35.0	37.0
8	21.926621846574935	35.0	0.0	37.0	0.0	37.0
9	29.845253554313725	37.0	18.0	39.0	17.0	39.0
10	34.24631157079396	35.0	32.0	39.0	27.0	39.0
11	35.90882784085233	37.0	35.0	39.0	32.0	39.0
12	36.50952726955589	38.0	35.0	39.0	32.0	39.0
13	36.70696452303313	39.0	35.0	39.0	33.0	39.0
14	37.88112551535584	40.0	37.0	41.0	33.0	41.0
15	38.10200280805869	40.0	37.0	41.0	33.0	41.0
16	38.12279248202878	40.0	37.0	41.0	33.0	41.0
17	38.07096052542845	40.0	37.0	41.0	33.0	41.0
18	37.955537549545404	39.0	36.0	41.0	33.0	41.0
19	37.89954775956162	40.0	37.0	41.0	33.0	41.0
20	37.72973778775883	39.0	35.0	41.0	33.0	41.0
21	37.64698980468831	39.0	35.0	41.0	33.0	41.0
22	37.619732164542704	39.0	35.0	41.0	33.0	41.0
23	37.438419698800466	39.0	35.0	41.0	33.0	41.0
24	37.23216783855032	39.0	35.0	41.0	33.0	41.0
25	37.09352234911501	39.0	35.0	41.0	32.0	41.0
26	37.02554735087703	39.0	35.0	41.0	32.0	41.0
27	36.905757635835656	39.0	35.0	41.0	32.0	41.0
28	36.88417797352271	39.0	35.0	41.0	32.0	41.0
29	36.757025344022686	38.0	35.0	40.0	31.0	41.0
30	36.59971980259419	38.0	35.0	40.0	31.0	41.0
31	36.39351053478357	38.0	35.0	40.0	30.0	41.0
32	35.92514733688237	38.0	35.0	40.0	29.0	41.0
33	35.531823397548195	38.0	35.0	41.0	25.0	41.0
34	35.1943016386427	39.0	35.0	41.0	23.0	41.0
35	34.8874839581154	38.0	35.0	41.0	21.0	41.0
36	34.71139210853365	38.0	34.0	40.0	19.0	41.0
37	34.675915648715815	38.0	34.0	40.0	19.0	41.0
38	34.57878054803286	38.0	34.0	40.0	18.0	41.0
39	34.491076123337564	38.0	34.0	40.0	18.0	41.0
40	34.45257813557681	38.0	34.0	40.0	18.0	41.0
41	34.4337461926669	38.0	34.0	40.0	18.0	41.0
42	34.1876217243752	38.0	34.0	40.0	17.0	41.0
43	34.22446621278676	38.0	34.0	40.0	17.0	41.0
44	34.03055956831548	38.0	33.0	40.0	15.0	41.0
45	33.84352501114249	38.0	33.0	40.0	15.0	41.0
46	33.90017853330906	38.0	33.0	40.0	15.0	41.0
47	33.7774550104681	38.0	33.0	40.0	15.0	41.0
48	33.775036875418635	38.0	33.0	40.0	15.0	41.0
49	33.70361898831801	38.0	33.0	40.0	15.0	41.0
50	33.248305809659456	37.0	32.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	0.0
9	0.0
10	0.0
11	2.0
12	2.0
13	2.0
14	6.0
15	28.0
16	100.0
17	296.0
18	856.0
19	1917.0
20	3531.0
21	6342.0
22	9616.0
23	14852.0
24	23156.0
25	35189.0
26	48150.0
27	50899.0
28	46750.0
29	44342.0
30	47827.0
31	57174.0
32	71425.0
33	91857.0
34	132222.0
35	192388.0
36	183413.0
37	266487.0
38	314925.0
39	328425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.497089770158013	23.362510844592865	27.776811560399373	19.36358782484975
2	36.1344115981241	23.84634067562764	27.69030834390961	12.328939382338639
3	25.20924803555049	23.826464203843358	38.08544956066406	12.878838199942095
4	22.64401695381915	25.805187252082845	38.003763346264876	13.547032447833136
5	21.103691801107505	30.092065586272255	36.255445113810545	12.548797498809694
6	18.309424946290427	38.1169375427509	33.478215234808566	10.09542227615011
7	80.50143470604371	3.2189743233506456	14.45719231652673	1.8223986540789106
8	49.73174368883992	40.177194689534076	8.366676283769086	1.7243853378569207
9	75.31560237118195	5.353413302328742	16.140557078685983	3.19042724780332
10	35.10722393127203	31.552884851846763	22.38942571701076	10.950465499870447
11	26.878871665430303	24.6581322911031	34.030395790244405	14.432600253222194
12	25.093335753665613	22.46908372000339	35.897465800552794	16.540114725778214
13	20.351681716840393	28.396785082099463	36.59481558741312	14.656717613647025
14	16.791612940191595	31.65226721076818	35.1316131734359	16.42450667560432
15	15.532397888429106	24.79468162404972	43.99286880869454	15.68005167882664
16	17.19046071329153	23.517364785483686	41.97165473148763	17.320519769737157
17	18.231592333563704	23.2513648595134	35.653573378914004	22.86346942800889
18	19.72222630681464	24.460635205389362	37.82558497419862	17.991553513597385
19	22.28710245154983	26.56034106403013	33.544841979514054	17.60771450490599
20	23.640933565428327	24.680493321860418	33.37462433721854	18.303948775492714
21	20.295905903160005	27.281116692636225	34.92402573597454	17.498951668229235
22	20.19363334298424	24.70300646847323	33.58505127064909	21.51830891789344
23	17.671400343071962	28.524207463716568	33.45524574062928	20.349146452582193
24	17.666329814555564	25.73856050737737	38.19035879566835	18.404750882398723
25	17.25587053115307	27.435970633527045	36.50592922252065	18.80222961279923
26	17.128752381246954	29.573401224329814	34.481672828203905	18.816173566219327
27	18.196605686800552	28.19842600653794	34.76577454097773	18.839193765683778
28	17.109890015165952	27.191773980177274	38.18153607604982	17.516799928606957
29	18.056354868036962	24.86627748670127	37.12813377676795	19.949233868493817
30	19.136580263170572	27.380194819846658	34.84888050336151	18.634344413621264
31	22.17484095019676	26.50633993533048	31.68989053235986	19.628928582112902
32	22.495349057718332	27.70136209607536	31.32019829822922	18.48309054797709
33	22.50685915745056	28.045194634772365	30.442084169759266	19.00586203801781
34	18.839295176254105	28.274585344854252	31.697090682853148	21.189028796038496
35	20.335760257298897	27.49397747975465	31.4524376819369	20.717824581009552
36	22.483078378708647	28.392323017005033	29.48111760533136	19.643480998954963
37	20.34047584881915	29.66183124165581	30.981436288048613	19.016256621476426
38	19.43711048833753	31.08614270191225	29.276775306120484	20.199971503629737
39	19.332556190329385	29.868049636417755	29.895531900976636	20.90386227227623
40	20.820452078181464	28.609747279788216	29.66862574986779	20.901174892162537
41	18.66557886928228	26.827862148555333	29.972603934425894	24.53395504773649
42	20.23242288613469	28.64478463183653	28.707202837873403	22.41558964415538
43	20.685474609074927	27.761853501275997	29.343097819114977	22.209574070534092
44	20.63644259832135	29.4676807047629	29.34117101827875	20.554705678637
45	19.046781203145148	30.568695266813744	29.041502782959576	21.343020747081532
46	20.99254581602804	30.424844372803513	28.769215401628955	19.81339440953949
47	19.945025329825203	28.287515192571068	30.116201302010314	21.651258175593416
48	20.573517339432843	27.00086858153486	30.311771586887815	22.113842492144485
49	20.58355698589531	25.445940306695984	31.245255886756844	22.725246820651858
50	19.351215735269733	28.19436958372482	30.885096246237033	21.569318434768412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1006.0
1	1404.0
2	1802.0
3	22992.0
4	44182.0
5	32843.0
6	21504.0
7	21849.0
8	22194.0
9	22948.5
10	23703.0
11	23968.0
12	24233.0
13	23978.0
14	23723.0
15	22690.0
16	21657.0
17	20445.5
18	19234.0
19	18403.5
20	17573.0
21	17200.5
22	16828.0
23	17362.0
24	17896.0
25	19827.0
26	21758.0
27	25009.5
28	28261.0
29	33158.5
30	38056.0
31	43064.5
32	48073.0
33	53133.0
34	58193.0
35	64510.5
36	70828.0
37	78898.0
38	86968.0
39	99451.0
40	111934.0
41	127455.5
42	142977.0
43	154202.0
44	165427.0
45	172523.0
46	179619.0
47	180033.5
48	180448.0
49	169235.0
50	158022.0
51	137474.0
52	116926.0
53	102936.0
54	88946.0
55	77831.5
56	66717.0
57	59007.5
58	51298.0
59	45048.5
60	38799.0
61	32406.5
62	26014.0
63	21156.0
64	16298.0
65	12597.0
66	8896.0
67	6952.5
68	5009.0
69	3894.5
70	2780.0
71	2335.0
72	1890.0
73	1511.5
74	1133.0
75	882.5
76	632.0
77	502.5
78	373.0
79	302.5
80	232.0
81	163.0
82	94.0
83	62.0
84	30.0
85	19.0
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1972181.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.20628474231254
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.85241231442731	30.843898154772738
2	8.300538295605442	6.84068689046375
3	3.959563312203603	4.894766798936276
4	2.46787005300235	4.067670244441631
5	1.8013288696483272	3.711303515863848
6	1.3757744168668615	3.401433141756293
7	1.0926107403752983	3.151570049628942
8	0.8571274573770857	2.825523045530764
9	0.7036586193190212	2.609564168613789
>10	4.501844316667544	32.497171073282956
>50	0.06848951724204663	1.809700341721599
>100	0.016418174131295617	1.2356089532474206
>500	0.00183859910410368	0.5415889120087536
>1k	2.6265701487195427E-4	0.16759600924171764
>5k	1.3132850743597714E-4	0.5369349705186705
>10k+	1.3132850743597714E-4	0.8649837299708151
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15984	0.8104732780611922	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9922	0.5030978393970938	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06713379755712078	0.0
2	0.0	0.0	0.0	0.20738461632071295	0.0
3	0.0	0.0	0.0	0.34616498181454947	0.0
4	0.0	0.0	0.0	0.4800269346474791	0.0
5	0.0	0.0	0.0	0.7791881171150112	0.0
6	0.0	0.0	0.0	1.2596713993289663	0.0
7	0.0	0.0	0.0	1.6101970356676187	0.0
8	0.0	0.0	0.0	2.4347663830043995	0.0
9	0.0	0.0	0.0	3.2563948238016693	0.0
10	0.0	0.0	0.0	4.422667087858569	0.0
11	0.0	0.0	0.0	5.035896806631846	0.0
12	0.0	0.0	0.0	5.664591637380139	0.0
13	0.0	0.0	0.0	6.033371176377828	0.0
14	0.0	0.0	0.0	6.20541420893924	0.0
15	0.0	0.0	0.0	6.369699332870563	0.0
16	0.0	0.0	0.0	6.673271875147362	0.0
17	0.0	0.0	0.0	6.999408269322136	0.0
18	0.0	0.0	0.0	7.574000560800454	0.0
19	0.0	0.0	0.0	7.786962758489206	0.0
20	0.0	0.0	0.0	8.053824674307277	0.0
21	0.0	0.0	0.0	8.277181455454647	0.0
22	0.0	0.0	0.0	8.515394885155064	0.0
23	0.0	0.0	0.0	8.789102014470274	0.0
24	0.0	0.0	0.0	8.999224716189843	0.0
25	0.0	0.0	0.0	9.184755354604876	0.0
26	0.0	0.0	0.0	9.36328866366728	0.0
27	0.0	0.0	0.0	9.57128174340996	0.0
28	0.0	0.0	0.0	9.774660642202718	0.0
29	0.0	0.0	0.0	9.98534110205909	0.0
30	0.0	0.0	0.0	10.258236946811676	0.0
31	0.0	0.0	0.0	10.486410730049625	0.0
32	0.0	0.0	0.0	10.70170537085592	0.0
33	0.0	0.0	0.0	10.917811296224839	0.0
34	5.0705285163988495E-5	0.0	0.0	11.130063619921295	0.0
35	5.0705285163988495E-5	0.0	0.0	11.362750173538839	0.0
36	5.0705285163988495E-5	0.0	0.0	11.587121060389487	0.0
37	5.0705285163988495E-5	0.0	0.0	11.820365372143835	0.0
38	5.0705285163988495E-5	0.0	0.0	12.064562025493604	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACCG	20	7.8578544E-4	44.0	12
TCGACGA	60	0.0	44.0	2
TCACGAC	495	0.0	43.555557	25
CGTTTTT	22005	0.0	43.190186	1
CGACGGT	505	0.0	43.128716	28
CACGACG	515	0.0	41.86408	26
CGGTCTA	515	0.0	41.86408	31
GAGCGAT	1335	0.0	41.528088	7
GACGGTC	535	0.0	40.71028	29
ACGGTCT	535	0.0	40.71028	30
TCTCACG	530	0.0	40.67925	23
GTTTTTA	9940	0.0	39.595573	2
TTTCGCG	40	4.1265412E-7	38.5	1
CTACGCG	40	4.1265412E-7	38.5	1
AATCGCG	40	4.1265412E-7	38.5	1
TCGCGAG	115	0.0	38.26087	3
CGCGAGT	35	7.2928615E-6	37.714287	4
TACGCGA	70	0.0	37.714287	2
GAGACCG	385	0.0	37.714287	7
TCGCGGA	35	7.2928615E-6	37.714287	2
>>END_MODULE