FastQCFastQC Report
Thu 26 May 2016
SRR1546784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546784_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1565833
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98600.6296967812020822No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60220.38458762843802624No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA16020.10230976100261012No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTA207.8573555E-444.00000429
TGCGCGA351.4465331E-744.02
CGTTTTT143050.043.1387631
CGACGGT2350.042.12766328
GAGCGAT10100.042.0396047
TAGTGCG850.041.4117661
TATTACG700.040.8571431
TGTACGA650.040.6153832
GTTTTTA63000.040.0190472
ACGTTAG557.8216544E-1140.01
TATACGA501.3478711E-939.62
AGCGATC1350.039.111118
TACGCGA404.1258136E-738.5000042
CGGACGA404.1258136E-738.5000042
TCACGAC2700.038.29629525
TGTTGCG750.038.133331
AGCGATA2500.037.848
TAATGCG651.0913936E-1137.2307661
GGCACCG3550.037.1830988
TTCGTAA301.3013104E-436.66666834
Produced by FastQC (version 0.11.5)