FastQCFastQC Report
Thu 26 May 2016
SRR1546783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546783_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2824608
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168200.5954808596449489No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103970.3680864743001507No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGA207.8584335E-444.02
CGCGTCA254.4444932E-544.039
CGTTTTT260750.043.130971
GAGCGAT16800.041.257
AACGCCG557.8216544E-1140.00000413
ATCGCGA557.8216544E-1140.0000042
GCGAACG1000.039.6000021
GCGATCG950.039.368429
GTTTTTA118750.039.3313672
ATTACGA900.039.111112
CGAGGTT2700.039.111116
CTATGCG700.037.7142871
TATACGA1050.037.7142872
CGGTCTA2950.037.28813631
AATCGCG1250.036.961
AGCGATA4050.036.9382748
GAGACCG5550.036.8648647
CGTACTA301.3015754E-436.66666823
TAAGCGA1200.036.6666682
TCGACAG1500.036.6666681
Produced by FastQC (version 0.11.5)