##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546783_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2824608
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.459315416510893	31.0	31.0	33.0	30.0	34.0
2	31.84988182430978	31.0	31.0	34.0	30.0	34.0
3	31.934051379872887	31.0	31.0	34.0	30.0	34.0
4	35.661939285026456	37.0	35.0	37.0	33.0	37.0
5	35.55590510258415	37.0	35.0	37.0	33.0	37.0
6	35.60102322162934	37.0	35.0	37.0	33.0	37.0
7	36.01693119894867	37.0	35.0	37.0	35.0	37.0
8	21.92316632962875	35.0	0.0	37.0	0.0	37.0
9	29.855596245567526	37.0	18.0	39.0	17.0	39.0
10	34.28840462110141	35.0	32.0	39.0	27.0	39.0
11	35.89696198552153	37.0	35.0	39.0	32.0	39.0
12	36.49003755565374	37.0	35.0	39.0	32.0	39.0
13	36.668657739410214	39.0	35.0	39.0	33.0	39.0
14	37.82939508774315	40.0	36.0	41.0	33.0	41.0
15	38.064703491599545	40.0	37.0	41.0	33.0	41.0
16	38.09010383033681	40.0	37.0	41.0	33.0	41.0
17	38.041321131994245	40.0	36.0	41.0	33.0	41.0
18	37.94596347528577	40.0	36.0	41.0	33.0	41.0
19	37.91952547043696	40.0	36.0	41.0	33.0	41.0
20	37.763405399970544	40.0	36.0	41.0	33.0	41.0
21	37.6665834692814	39.0	35.0	41.0	33.0	41.0
22	37.632912956417314	39.0	35.0	41.0	33.0	41.0
23	37.46023023371739	39.0	35.0	41.0	33.0	41.0
24	37.29642697322956	39.0	35.0	41.0	32.0	41.0
25	37.156327532882436	39.0	35.0	41.0	32.0	41.0
26	37.09941450282658	39.0	35.0	41.0	32.0	41.0
27	36.985614641040456	39.0	35.0	41.0	32.0	41.0
28	36.97139496878859	39.0	35.0	41.0	32.0	41.0
29	36.86327483318039	39.0	35.0	41.0	31.0	41.0
30	36.71531023065855	39.0	35.0	41.0	31.0	41.0
31	36.55786325040501	39.0	35.0	41.0	31.0	41.0
32	36.136439817489716	39.0	35.0	41.0	30.0	41.0
33	35.817428825521986	39.0	35.0	41.0	27.0	41.0
34	35.54892962138463	39.0	35.0	41.0	25.0	41.0
35	35.29645919008939	39.0	35.0	41.0	24.0	41.0
36	35.13756457533222	39.0	35.0	41.0	23.0	41.0
37	35.11201200308149	39.0	35.0	41.0	23.0	41.0
38	35.01899555619753	39.0	35.0	40.0	23.0	41.0
39	34.93446913695635	38.0	35.0	40.0	22.0	41.0
40	34.89702854342974	38.0	34.0	40.0	22.0	41.0
41	34.884579382342615	38.0	34.0	40.0	22.0	41.0
42	34.63407488755962	38.0	34.0	40.0	20.0	41.0
43	34.672138222365724	38.0	34.0	40.0	21.0	41.0
44	34.4721904774043	38.0	34.0	40.0	19.0	41.0
45	34.27152227848962	38.0	34.0	40.0	18.0	41.0
46	34.3456461923212	38.0	34.0	40.0	20.0	41.0
47	34.22975577496063	38.0	33.0	40.0	19.0	41.0
48	34.23152522403109	38.0	34.0	40.0	19.0	41.0
49	34.15770294497502	38.0	33.0	40.0	18.0	41.0
50	33.70025469020834	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	6.0
13	4.0
14	10.0
15	58.0
16	149.0
17	429.0
18	1135.0
19	2441.0
20	4653.0
21	8225.0
22	12636.0
23	19189.0
24	28942.0
25	44051.0
26	59198.0
27	64905.0
28	62007.0
29	61828.0
30	67376.0
31	81328.0
32	102640.0
33	132115.0
34	192097.0
35	278148.0
36	261770.0
37	389892.0
38	464889.0
39	484485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.23215964834766	23.363489730256376	28.504946527093317	17.899404094302643
2	34.69511521598749	24.046770383713422	28.333843138587728	12.92427126171136
3	26.040108928389355	24.01427029874588	36.92908892136537	13.016531851499394
4	23.261387066807146	26.55572029817943	36.59852977829136	13.584362856722066
5	21.75066416295642	30.618337128550227	34.81484864448448	12.816150064008882
6	19.00777028175237	38.87229661602601	31.62187461056543	10.498058491656186
7	83.13330557726948	3.1061301249589324	12.062240140932829	1.6983241568387542
8	51.234826213053275	40.006365485051376	6.9942094619855215	1.7645988399098211
9	78.05631790322764	5.073907600629893	13.889891977931097	2.9798825182113764
10	37.172166898911286	33.64997904133954	18.798466902309986	10.379387157439192
11	26.891058865513372	24.041353702885498	34.46662333322004	14.600964098381086
12	26.293701639307116	22.240572851170853	34.60607631218208	16.859649197339948
13	20.10838318095821	28.618307389910385	35.55158804336743	15.72172138576397
14	16.59908206731695	31.43016659302813	34.6187152341139	17.352036105541018
15	15.218217890765729	24.83378224518234	43.95887854173039	15.98912132232154
16	17.913211319942448	23.439889712129965	41.69934376734754	16.947555200580044
17	18.237539509907215	23.32327176018761	34.78241936580226	23.65676936410291
18	20.06494352490682	24.377860573927425	36.94689670212645	18.6102991990393
19	22.612624477449614	26.466044137806026	32.852346237070776	18.068985147673587
20	23.989842130306226	25.182892635013427	32.18156289297489	18.64570234170547
21	21.32950837779968	26.987886460705347	33.557824660979506	18.12478050051547
22	20.724964313632192	24.608405838969514	32.778672297182474	21.887957550215816
23	17.83928955805549	28.448974158538103	32.66895795806002	21.042778325346383
24	18.262746547485527	25.388514087618532	37.76690429256024	18.5818350723357
25	17.746922758839457	26.79897529143867	35.62784641267036	19.826255537051512
26	17.809126080503916	29.276416408931787	33.654935481312805	19.259522029251492
27	18.282713919949245	28.62846809185558	33.92403476871835	19.164783219476828
28	17.234356059318674	27.488982542002287	37.55154697572194	17.7251144229571
29	18.176823120234737	24.974297318424362	36.17882552198394	20.670054039356966
30	19.833442375012744	26.77922033783095	33.725033703791816	19.662303583364487
31	23.15177893711269	25.94568874689869	30.38410993667086	20.518422379317766
32	23.559446124913617	26.56173883243268	30.10821324587341	19.770601796780298
33	23.052579331362086	27.1804795568093	30.026148761173232	19.740792350655383
34	19.814678709399676	28.31840736838528	30.63961441729259	21.227299504922453
35	21.117160328088	27.15937928378026	30.60555659404774	21.117903794083993
36	23.084902400616297	28.14255995876242	29.655194632317123	19.11734300830416
37	20.95550957867428	29.044986065323048	30.460722337400448	19.538782018602227
38	19.806111148874464	29.968300026056717	29.713361995717634	20.51222682935119
39	19.43211234974906	28.690458994664038	31.08640207773964	20.791026577847262
40	21.06058610610747	26.528035040614483	31.18312346350361	21.22825538977444
41	18.4941414879516	25.926748065572287	31.23778591578017	24.34132453069594
42	20.99505488903239	26.982221957878753	29.623190191346904	22.399532961741947
43	21.23073360976107	26.80906518709853	30.249790413395417	21.710410789744984
44	20.646227724342634	29.468443054753084	29.478143515843612	20.40718570506067
45	19.36512960382467	30.456721782279168	28.75344118546715	21.424707428429006
46	21.404563040251958	29.440899409758803	28.935023904201927	20.21951364578731
47	20.585405125242158	26.83777713580079	30.043602510507654	22.5332152284494
48	21.226733054639794	25.595268440788953	30.778819574255966	22.399178930315287
49	20.75353464976379	25.227146563346135	31.21126896192321	22.808049824966865
50	19.63844186520749	27.617177321596483	30.819993429176723	21.924387384019305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1465.0
1	1981.5
2	2498.0
3	26431.5
4	50365.0
5	38158.5
6	25952.0
7	26433.5
8	26915.0
9	27756.5
10	28598.0
11	28850.5
12	29103.0
13	28387.0
14	27671.0
15	26737.5
16	25804.0
17	24407.0
18	23010.0
19	22069.5
20	21129.0
21	20825.5
22	20522.0
23	21813.5
24	23105.0
25	26445.0
26	29785.0
27	34770.0
28	39755.0
29	46837.5
30	53920.0
31	62130.0
32	70340.0
33	78145.0
34	85950.0
35	95893.0
36	105836.0
37	116643.5
38	127451.0
39	145038.5
40	162626.0
41	185904.5
42	209183.0
43	224368.0
44	239553.0
45	250021.5
46	260490.0
47	261498.0
48	262506.0
49	246345.0
50	230184.0
51	202633.5
52	175083.0
53	152110.0
54	129137.0
55	113759.5
56	98382.0
57	87641.0
58	76900.0
59	67866.5
60	58833.0
61	49570.5
62	40308.0
63	32796.5
64	25285.0
65	19488.5
66	13692.0
67	11174.5
68	8657.0
69	7437.0
70	6217.0
71	4954.5
72	3692.0
73	2872.5
74	2053.0
75	1625.0
76	1197.0
77	939.5
78	682.0
79	528.0
80	374.0
81	324.0
82	274.0
83	182.5
84	91.0
85	53.0
86	15.0
87	13.5
88	12.0
89	7.0
90	2.0
91	2.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2824608.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.07752420833811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.27362986644201	25.992164682314982
2	7.043555819094263	4.80053887875931
3	3.1040738138937103	3.17337451612294
4	1.9031220072504689	2.5941474509399547
5	1.434736533966531	2.4446134484415785
6	1.1095493255133797	2.2686416400316447
7	0.9252808118091655	2.2071895484756308
8	0.8036632487984183	2.19094830530238
9	0.6979462876841586	2.1405853363208966
>10	6.364312795528179	42.6319563430479
>50	0.3154867718240912	6.695823504265194
>100	0.022783679283388463	1.307272464852079
>500	0.0010935523013568171	0.26730582617689896
>1k	5.467761506783946E-4	0.27118144389924076
>5k	0.0	0.0
>10k+	2.187104602713579E-4	1.0142566110492834
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16820	0.5954808596449489	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10397	0.3680864743001507	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05696365654986462	0.0
2	0.0	0.0	0.0	0.1729443519242316	0.0
3	0.0	0.0	0.0	0.2737370990948125	0.0
4	0.0	0.0	0.0	0.40037414041169606	0.0
5	0.0	0.0	0.0	0.65885248501739	0.0
6	0.0	0.0	0.0	1.1316260521814	0.0
7	0.0	0.0	0.0	1.493233751373642	0.0
8	0.0	0.0	0.0	2.423628340640542	0.0
9	0.0	0.0	0.0	3.3582005007420497	0.0
10	0.0	0.0	0.0	4.56254460795976	0.0
11	3.540314266616819E-5	0.0	0.0	5.171018420963192	0.0
12	3.540314266616819E-5	0.0	0.0	5.777474254834654	0.0
13	3.540314266616819E-5	0.0	0.0	6.123823199537776	0.0
14	3.540314266616819E-5	0.0	0.0	6.277756063850276	0.0
15	3.540314266616819E-5	0.0	0.0	6.418271137092297	0.0
16	3.540314266616819E-5	0.0	0.0	6.671828444867394	0.0
17	3.540314266616819E-5	0.0	0.0	6.948964245658159	0.0
18	3.540314266616819E-5	0.0	0.0	7.412532995728965	0.0
19	3.540314266616819E-5	0.0	0.0	7.5995677984343315	0.0
20	7.080628533233638E-5	0.0	0.0	7.834680068880354	0.0
21	7.080628533233638E-5	0.0	0.0	8.055489469689245	0.0
22	7.080628533233638E-5	0.0	0.0	8.287380054152647	0.0
23	7.080628533233638E-5	0.0	0.0	8.552762011578244	0.0
24	7.080628533233638E-5	0.0	0.0	8.754382909062072	0.0
25	7.080628533233638E-5	0.0	0.0	8.936178046652845	0.0
26	7.080628533233638E-5	0.0	0.0	9.107352241443769	0.0
27	7.080628533233638E-5	0.0	0.0	9.302812992103682	0.0
28	7.080628533233638E-5	0.0	0.0	9.496893020199616	0.0
29	7.080628533233638E-5	0.0	0.0	9.704036807939367	0.0
30	7.080628533233638E-5	0.0	0.0	9.96743618937566	0.0
31	1.0620942799850457E-4	0.0	0.0	10.190405181887186	0.0
32	1.0620942799850457E-4	0.0	0.0	10.397761388482932	0.0
33	1.0620942799850457E-4	0.0	0.0	10.611454757615924	0.0
34	1.0620942799850457E-4	0.0	0.0	10.821289184198303	0.0
35	1.0620942799850457E-4	0.0	0.0	11.05774677406564	0.0
36	1.4161257066467277E-4	0.0	0.0	11.275086666893246	0.0
37	1.4161257066467277E-4	0.0	0.0	11.510623775051263	0.0
38	1.4161257066467277E-4	0.0	0.0	11.74357645379465	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCGA	20	7.8584335E-4	44.0	2
CGCGTCA	25	4.4444932E-5	44.0	39
CGTTTTT	26075	0.0	43.13097	1
GAGCGAT	1680	0.0	41.25	7
AACGCCG	55	7.8216544E-11	40.000004	13
ATCGCGA	55	7.8216544E-11	40.000004	2
GCGAACG	100	0.0	39.600002	1
GCGATCG	95	0.0	39.36842	9
GTTTTTA	11875	0.0	39.331367	2
ATTACGA	90	0.0	39.11111	2
CGAGGTT	270	0.0	39.11111	6
CTATGCG	70	0.0	37.714287	1
TATACGA	105	0.0	37.714287	2
CGGTCTA	295	0.0	37.288136	31
AATCGCG	125	0.0	36.96	1
AGCGATA	405	0.0	36.938274	8
GAGACCG	555	0.0	36.864864	7
CGTACTA	30	1.3015754E-4	36.666668	23
TAAGCGA	120	0.0	36.666668	2
TCGACAG	150	0.0	36.666668	1
>>END_MODULE