FastQCFastQC Report
Thu 26 May 2016
SRR1546782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546782_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1320286
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45680.34598564250473No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27410.20760653373587237No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16290.12338235806484353No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT207.856906E-444.00000413
TCGAGTA207.856906E-444.00000441
TATACGA408.314601E-944.0000042
TTCGATC207.856906E-444.00000428
TCGACCA254.4432003E-544.044
CGGTCTA5350.042.76635431
TCACGAC5700.041.6842125
CGACGGT5600.041.2528
CGTTTTT40400.041.004951
CACGACG5700.040.9122826
CGACAGA700.040.8571432
GACGGTC5600.040.85714329
GCGATAT1050.039.8095259
ACGGTCT5750.039.79130630
TCTAGCG501.3478711E-939.6000021
GAGCGAT8000.039.6000027
ACATACG4450.039.55056417
CTCACGA6150.038.9918724
TCTAAAC2900.038.68965534
TCTCACG6150.038.63414823
Produced by FastQC (version 0.11.5)