##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546782_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1320286
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394478166094316	31.0	31.0	33.0	30.0	34.0
2	31.788992687947914	31.0	31.0	34.0	30.0	34.0
3	31.93667811368143	31.0	31.0	34.0	30.0	34.0
4	35.63961293234951	37.0	35.0	37.0	33.0	37.0
5	35.51299415429687	37.0	35.0	37.0	33.0	37.0
6	35.56449360214378	37.0	35.0	37.0	33.0	37.0
7	35.93118536438317	37.0	35.0	37.0	35.0	37.0
8	22.23640408214584	35.0	0.0	37.0	0.0	37.0
9	29.992703853559	37.0	18.0	39.0	17.0	39.0
10	34.27030582767673	35.0	32.0	39.0	27.0	39.0
11	35.88379260251188	37.0	35.0	39.0	32.0	39.0
12	36.14492920473291	37.0	35.0	39.0	32.0	39.0
13	36.15947983997407	38.0	35.0	39.0	32.0	39.0
14	37.223933299300306	39.0	35.0	41.0	32.0	41.0
15	37.5316582922185	39.0	35.0	41.0	33.0	41.0
16	37.63278789595588	39.0	35.0	41.0	33.0	41.0
17	37.598515018715645	39.0	35.0	41.0	33.0	41.0
18	37.573134154266576	39.0	36.0	41.0	33.0	41.0
19	37.57426875692085	39.0	36.0	41.0	33.0	41.0
20	37.45344720765046	39.0	35.0	41.0	32.0	41.0
21	37.34161234762771	39.0	35.0	41.0	32.0	41.0
22	37.27842528058314	39.0	35.0	41.0	32.0	41.0
23	37.16027739444333	39.0	35.0	41.0	32.0	41.0
24	37.06737176642031	39.0	35.0	41.0	32.0	41.0
25	36.92275007081799	38.0	35.0	41.0	32.0	41.0
26	36.85776187886564	38.0	35.0	41.0	32.0	41.0
27	36.74157871855037	38.0	35.0	40.0	31.0	41.0
28	36.76479793014544	38.0	35.0	40.0	31.0	41.0
29	36.67359344869218	38.0	35.0	40.0	31.0	41.0
30	36.60774786675008	38.0	35.0	40.0	31.0	41.0
31	36.52342295532937	38.0	35.0	40.0	31.0	41.0
32	36.26095255118967	38.0	35.0	40.0	30.0	41.0
33	36.16231634660975	38.0	35.0	40.0	30.0	41.0
34	36.079999333477744	38.0	35.0	40.0	30.0	41.0
35	35.93733857664173	38.0	35.0	40.0	30.0	41.0
36	35.800320536611004	38.0	35.0	40.0	29.0	41.0
37	35.771026126157516	38.0	35.0	40.0	29.0	41.0
38	35.71585095956482	38.0	35.0	40.0	29.0	41.0
39	35.62702853775621	38.0	35.0	40.0	28.0	41.0
40	35.53261111607637	38.0	35.0	40.0	28.0	41.0
41	35.53006469810329	38.0	35.0	40.0	28.0	41.0
42	35.30868236124597	38.0	34.0	40.0	27.0	41.0
43	35.326380799311664	38.0	34.0	40.0	27.0	41.0
44	35.12265069840928	37.0	34.0	40.0	27.0	41.0
45	34.915672058932685	37.0	34.0	40.0	26.0	41.0
46	34.97858267072437	37.0	34.0	40.0	26.0	41.0
47	34.82677389595891	37.0	34.0	40.0	26.0	41.0
48	34.859323661691484	37.0	34.0	40.0	26.0	41.0
49	34.78998035274176	37.0	34.0	40.0	26.0	41.0
50	34.29484596519239	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	2.0
13	5.0
14	8.0
15	25.0
16	64.0
17	181.0
18	492.0
19	1004.0
20	1833.0
21	3228.0
22	4889.0
23	7173.0
24	9814.0
25	13548.0
26	17565.0
27	20292.0
28	23008.0
29	26638.0
30	32456.0
31	41201.0
32	53211.0
33	70470.0
34	110921.0
35	176728.0
36	126739.0
37	181498.0
38	210321.0
39	186971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.889075548782614	25.476146834852447	29.606236830504905	13.028540785860033
2	30.168160534914406	26.33421849508364	29.85792472237076	13.639696247631194
3	27.940537126046934	25.729501032352083	32.138112499867454	14.191849341733533
4	25.612935379152702	27.719903111901512	31.493555184255534	15.173606324690256
5	22.98108137176339	32.672314937824076	29.81225279977217	14.53435089064036
6	19.88061677545623	41.6399174118335	27.48737773482412	10.992088077886155
7	86.55192890025343	4.787750532839096	6.608643884734065	2.051676682173408
8	54.23529447407607	39.866816735161926	3.7573677218420856	2.1405210689199157
9	81.90717768725867	5.778142008625404	8.566098557433769	3.7485817466821585
10	48.346191658474	25.51522927608109	13.854801156719073	12.283777908725837
11	42.6958249954934	21.927446023058643	21.358478390288166	14.018250591159795
12	39.00942674541728	21.28364611909844	24.192485567520976	15.514441567963305
13	23.848620677641055	37.481121514580934	24.041987872324633	14.628269935453378
14	16.475445471662958	40.59885509654727	26.485549343096874	16.440150088692903
15	14.62274083039584	23.115824904604	45.46916349942361	16.79227076557655
16	15.626992939408582	19.412839339355262	43.35507609714865	21.605091624087507
17	16.38569219093439	20.33650284862522	28.889498184484268	34.38830677595612
18	21.310231268073736	23.686686066503775	32.74502645638899	22.258056209033498
19	27.35687570723313	26.166679037723643	24.632390254838725	21.844055000204502
20	29.708790368147508	23.913227891532593	23.82544388109849	22.55253785922141
21	22.779837095901946	29.508833692093983	25.79766808100669	21.913661130997376
22	22.67773800525038	26.130323278441185	23.874221191469122	27.317717524839313
23	19.62544478999247	31.461289447892348	23.656238118104714	25.257027644010467
24	20.314083463734374	25.347538336390752	34.982571957893974	19.355806241980904
25	18.505157215936546	26.166376073062956	32.144777722402566	23.183688988597925
26	17.691697101991537	32.28255090185005	26.972186329325616	23.053565666832792
27	19.259311997552047	31.715779762869563	27.04179246011849	21.983115779459904
28	17.042671057634482	28.529348944092416	34.81109395994505	19.61688603832806
29	17.356618187271547	24.42819207353558	34.318397680502564	23.896792058690313
30	20.326883720648407	29.272445515592832	29.53148030048035	20.86919046327841
31	27.50502542630915	26.41420116550505	23.236632063053005	22.844141345132797
32	28.112090865161033	26.073517404562345	24.33866601630253	21.475725713974096
33	27.22894887925798	26.916895278750207	23.538990794418783	22.315165047573025
34	19.997030946325268	26.153575816148926	27.65582608616618	26.19356715135963
35	20.941371793687125	24.446748659002672	29.754765255406785	24.85711429190342
36	27.30612912656803	24.289737223601556	26.74708358643506	21.657050063395356
37	20.51434310444858	30.271320001878383	29.17193698940987	20.042399904263167
38	20.63719527435722	29.72310544836498	25.314363706045505	24.325335571232294
39	20.45155367852117	30.108552237924208	26.635138144311156	22.804755939243467
40	22.907157994555725	25.074491435946456	26.588860292391193	25.429490277106627
41	18.190831380473625	24.59073261399424	26.99922592529194	30.219210080240188
42	22.05370654540001	26.138351841949397	23.803327460868324	28.004614151782263
43	22.50254869020803	26.346034116850443	25.41737169067914	25.734045502262386
44	21.139510681776525	28.884196302922245	27.100870568952484	22.875422446348743
45	18.21135723623518	32.4754636495426	24.54528791489117	24.767891199331054
46	22.71416950569801	29.915715231396835	26.555382697385266	20.814732565519893
47	21.367037141952576	26.738979281761676	27.334683545837795	24.559300030447947
48	23.049854349739373	23.484305673164755	28.767251943897005	24.698588033198867
49	21.606227741565085	22.83959687522249	29.98827526763141	25.56590011558102
50	19.576515997291498	28.914871474816824	27.682335493976307	23.82627703391538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	824.0
1	1139.5
2	1455.0
3	4675.5
4	7896.0
5	6056.5
6	4217.0
7	4491.5
8	4766.0
9	5320.5
10	5875.0
11	6130.5
12	6386.0
13	6344.0
14	6302.0
15	6064.0
16	5826.0
17	5605.0
18	5384.0
19	5153.0
20	4922.0
21	5004.5
22	5087.0
23	5424.5
24	5762.0
25	6829.5
26	7897.0
27	9219.5
28	10542.0
29	12423.0
30	14304.0
31	17325.5
32	20347.0
33	23617.5
34	26888.0
35	29849.5
36	32811.0
37	37330.0
38	41849.0
39	55962.5
40	70076.0
41	87117.0
42	104158.0
43	114660.5
44	125163.0
45	128854.0
46	132545.0
47	130111.0
48	127677.0
49	122450.0
50	117223.0
51	103894.0
52	90565.0
53	79601.0
54	68637.0
55	63232.5
56	57828.0
57	55064.5
58	52301.0
59	48426.5
60	44552.0
61	40304.0
62	36056.0
63	30616.0
64	25176.0
65	20435.5
66	15695.0
67	13485.0
68	11275.0
69	9841.0
70	8407.0
71	7004.5
72	5602.0
73	4626.0
74	3650.0
75	2808.0
76	1966.0
77	1591.5
78	1217.0
79	958.5
80	700.0
81	492.0
82	284.0
83	203.0
84	122.0
85	83.5
86	45.0
87	30.5
88	16.0
89	10.0
90	4.0
91	4.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1320286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.858481071225423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.78382053609415	22.627897709821116
2	6.726738364283502	4.017003802420896
3	2.8993270071541084	2.597085016872108
4	1.7813830222115343	2.127575649972217
5	1.3515680069240186	2.017788387560734
6	1.0421815150360176	1.867077422369038
7	0.871000834257484	1.8204733345889048
8	0.7471926924710339	1.784803109176346
9	0.6472153123935983	1.739237953870035
>10	7.6848505650654	48.59657663191323
>50	0.4196623082237858	7.876208743150857
>100	0.04301166155423268	1.9929772295680055
>500	0.0012801089569685433	0.25203658714659455
>1k	7.68065374181126E-4	0.6832584215699915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4568	0.34598564250473	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2741	0.20760653373587237	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1629	0.12338235806484353	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05832069718227717	0.0
2	0.0	0.0	0.0	0.1902618069115328	0.0
3	0.0	0.0	0.0	0.2978142614554725	0.0
4	0.0	0.0	0.0	0.4625512957041126	0.0
5	0.0	0.0	0.0	0.7656674387216104	0.0
6	0.0	0.0	0.0	1.1806532826978398	0.0
7	0.0	0.0	0.0	1.47922495580503	0.0
8	0.0	0.0	0.0	2.3411594154599835	0.0
9	0.0	0.0	0.0	3.060851966922318	0.0
10	7.574116517178854E-5	0.0	0.0	4.149101028110576	0.0
11	7.574116517178854E-5	0.0	0.0	4.787599050508754	0.0
12	7.574116517178854E-5	0.0	0.0	5.444956623034706	0.0
13	7.574116517178854E-5	0.0	0.0	5.748451471878063	0.0
14	7.574116517178854E-5	0.0	0.0	5.866986395371912	0.0
15	7.574116517178854E-5	0.0	0.0	6.009228303564531	0.0
16	7.574116517178854E-5	0.0	0.0	6.29590861373975	0.0
17	7.574116517178854E-5	0.0	0.0	6.604478120649617	0.0
18	7.574116517178854E-5	0.0	0.0	7.127016419169786	0.0
19	7.574116517178854E-5	0.0	0.0	7.321519731330939	0.0
20	7.574116517178854E-5	0.0	0.0	7.571465576397841	0.0
21	7.574116517178854E-5	0.0	0.0	7.8212599391344	0.0
22	7.574116517178854E-5	0.0	0.0	8.060904985737938	0.0
23	7.574116517178854E-5	0.0	0.0	8.336375603467733	0.0
24	7.574116517178854E-5	0.0	0.0	8.529667056986138	0.0
25	7.574116517178854E-5	0.0	0.0	8.694101126574092	0.0
26	7.574116517178854E-5	0.0	0.0	8.851794232461755	0.0
27	7.574116517178854E-5	0.0	0.0	9.031073570423377	0.0
28	7.574116517178854E-5	0.0	0.0	9.19974914526095	0.0
29	7.574116517178854E-5	0.0	0.0	9.383345729637366	0.0
30	7.574116517178854E-5	0.0	0.0	9.614659248072009	0.0
31	7.574116517178854E-5	0.0	0.0	9.813934253638985	0.0
32	7.574116517178854E-5	0.0	0.0	9.997152132189541	0.0
33	7.574116517178854E-5	0.0	0.0	10.18302095152111	0.0
34	7.574116517178854E-5	0.0	0.0	10.362830477638935	0.0
35	7.574116517178854E-5	0.0	0.0	10.579980398186454	0.0
36	7.574116517178854E-5	0.0	0.0	10.77554408666001	0.0
37	7.574116517178854E-5	0.0	0.0	10.96648756405809	0.0
38	7.574116517178854E-5	0.0	0.0	11.166595722441956	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGT	20	7.856906E-4	44.000004	13
TCGAGTA	20	7.856906E-4	44.000004	41
TATACGA	40	8.314601E-9	44.000004	2
TTCGATC	20	7.856906E-4	44.000004	28
TCGACCA	25	4.4432003E-5	44.0	44
CGGTCTA	535	0.0	42.766354	31
TCACGAC	570	0.0	41.68421	25
CGACGGT	560	0.0	41.25	28
CGTTTTT	4040	0.0	41.00495	1
CACGACG	570	0.0	40.91228	26
CGACAGA	70	0.0	40.857143	2
GACGGTC	560	0.0	40.857143	29
GCGATAT	105	0.0	39.809525	9
ACGGTCT	575	0.0	39.791306	30
TCTAGCG	50	1.3478711E-9	39.600002	1
GAGCGAT	800	0.0	39.600002	7
ACATACG	445	0.0	39.550564	17
CTCACGA	615	0.0	38.99187	24
TCTAAAC	290	0.0	38.689655	34
TCTCACG	615	0.0	38.634148	23
>>END_MODULE