FastQCFastQC Report
Thu 26 May 2016
SRR1546781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546781_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1695896
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131920.7778778887384603No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81540.480807785383066No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17090.10077268889129995No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA207.8575424E-444.029
GATCGCG254.4437387E-544.01
CCGCTCG454.802132E-1044.019
CGTTTTT191950.043.2091671
GAGCGAT10500.042.1142857
GCGTTAG900.041.5555571
CGACGGT4400.041.528
CGGTCTA4450.041.03370731
TCACGAC4550.040.1318725
ACGCCGA557.8216544E-1140.012
GACGGTC4650.039.74193229
CGCAGAG2550.039.686273
TTGCGGA501.3478711E-939.62
GTTTTTA81850.039.5650562
ACGGTCT4650.039.26881430
TACGCGA404.1260864E-738.52
CTACGCG404.1260864E-738.51
TCTCACG4800.038.04166423
ACGAGGT1450.037.9310345
CGTTAGA1000.037.3999982
Produced by FastQC (version 0.11.5)