FastQCFastQC Report
Thu 26 May 2016
SRR1546780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546780_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1938770
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141280.7287094394899859No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87000.4487381174662286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGA207.8578206E-444.0000042
TGCGCGA502.7284841E-1144.02
ACGTACG254.4439745E-544.01
CGTTTTT204600.043.333331
GAGCGAT11250.041.653337
CGACGGT1350.040.7407428
GTTAGCG557.8216544E-1140.01
GTTTTTA85600.039.6822432
ACGTGCG501.3496901E-939.6000021
ATTACGA750.038.133332
TATAGCG1450.037.9310341
GCGCGAC3250.037.9076929
AGACCGA3350.037.4328358
AATGCGG1600.037.1250042
GCGAGGT2850.037.0526285
CGCGAGA1550.036.9032254
CGAGGAT2150.036.8372086
CGGTCTA1500.036.66666831
GCGCGAG2100.036.6666643
AACGCGA301.3014252E-436.6666642
Produced by FastQC (version 0.11.5)