##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546780_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1938770
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.479112014318357	31.0	31.0	33.0	30.0	34.0
2	31.87574544685548	31.0	31.0	34.0	30.0	34.0
3	31.950338616751857	31.0	31.0	34.0	30.0	34.0
4	35.67643505934175	37.0	35.0	37.0	33.0	37.0
5	35.569433713127395	37.0	35.0	37.0	33.0	37.0
6	35.62103653347225	37.0	35.0	37.0	33.0	37.0
7	36.0248987760281	37.0	35.0	37.0	35.0	37.0
8	22.305506584071345	35.0	0.0	37.0	0.0	37.0
9	30.069767945656267	37.0	18.0	39.0	17.0	39.0
10	34.36688673746757	35.0	32.0	39.0	27.0	39.0
11	35.93229986022065	37.0	35.0	39.0	32.0	39.0
12	36.52007974127927	38.0	35.0	39.0	32.0	39.0
13	36.72019631003162	39.0	35.0	39.0	33.0	39.0
14	37.9108935046447	40.0	37.0	41.0	33.0	41.0
15	38.13059775011992	40.0	37.0	41.0	33.0	41.0
16	38.14945609845417	40.0	37.0	41.0	33.0	41.0
17	38.09518096525117	40.0	37.0	41.0	33.0	41.0
18	37.99029281451642	40.0	37.0	41.0	33.0	41.0
19	37.9512531140878	40.0	37.0	41.0	33.0	41.0
20	37.782690571857415	40.0	36.0	41.0	33.0	41.0
21	37.705785111178734	40.0	35.0	41.0	33.0	41.0
22	37.67754658881662	39.0	35.0	41.0	33.0	41.0
23	37.49471984815115	39.0	35.0	41.0	33.0	41.0
24	37.30229320651753	39.0	35.0	41.0	33.0	41.0
25	37.163447443482205	39.0	35.0	41.0	32.0	41.0
26	37.08966406536103	39.0	35.0	41.0	32.0	41.0
27	36.9726873223745	39.0	35.0	41.0	32.0	41.0
28	36.94668991164501	39.0	35.0	41.0	32.0	41.0
29	36.845391665849995	39.0	35.0	41.0	31.0	41.0
30	36.684670693274605	39.0	35.0	41.0	31.0	41.0
31	36.50281312378467	39.0	35.0	41.0	31.0	41.0
32	36.03055803421757	39.0	35.0	41.0	29.0	41.0
33	35.64597038328425	39.0	35.0	41.0	25.0	41.0
34	35.32373050954987	39.0	35.0	41.0	23.0	41.0
35	35.02252562191492	39.0	35.0	41.0	22.0	41.0
36	34.85368300520433	39.0	35.0	41.0	21.0	41.0
37	34.812937584138396	39.0	34.0	41.0	20.0	41.0
38	34.706137396390496	38.0	34.0	40.0	20.0	41.0
39	34.60718238883416	38.0	34.0	40.0	18.0	41.0
40	34.53344543189754	38.0	34.0	40.0	18.0	41.0
41	34.510892988853755	38.0	34.0	40.0	18.0	41.0
42	34.26805964606426	38.0	34.0	40.0	18.0	41.0
43	34.306944609210994	38.0	34.0	40.0	18.0	41.0
44	34.118387431206386	38.0	33.0	40.0	17.0	41.0
45	33.92221717893303	38.0	33.0	40.0	17.0	41.0
46	34.00704725160798	38.0	33.0	40.0	17.0	41.0
47	33.90071643361513	38.0	33.0	40.0	17.0	41.0
48	33.895434734393454	38.0	33.0	40.0	17.0	41.0
49	33.80639064974185	38.0	33.0	40.0	16.0	41.0
50	33.33946264899911	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	7.0
14	9.0
15	28.0
16	124.0
17	327.0
18	816.0
19	1928.0
20	3637.0
21	6124.0
22	9655.0
23	14579.0
24	21758.0
25	33681.0
26	44819.0
27	48684.0
28	45656.0
29	43674.0
30	46893.0
31	54916.0
32	68715.0
33	87021.0
34	126520.0
35	184989.0
36	175858.0
37	262809.0
38	316066.0
39	339473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.854546955028187	23.02098753333299	27.424294784837812	19.700170726801012
2	35.63114758326155	24.310413303279915	27.40603578557539	12.65240332788314
3	26.0363013663302	23.58082701919258	37.835380163712045	12.547491450765175
4	22.961207363431456	26.183611258684632	37.38483677795716	13.47034459992676
5	21.253217245985855	30.02960639993398	36.108099465124795	12.609076888955368
6	18.690561541595958	38.508693656287235	32.61335795375418	10.187386848362621
7	81.58260134002487	3.2835767006916754	13.473645661940303	1.6601762973431609
8	51.27513836091955	39.18654610913105	7.924663575359634	1.6136519545897656
9	76.80550039458008	5.223260108212939	15.077652325959242	2.89358717124775
10	36.76093605739722	31.050975618562283	21.328522723169847	10.859565600870654
11	26.769033975149192	24.624787880976083	34.215868824048236	14.390309319826489
12	25.62769178396612	22.627903258251365	35.33714674768023	16.40725821010228
13	20.338771489140022	28.66915621760188	35.75942478994414	15.232647503313956
14	16.79735089773413	31.217318196588558	35.16837995223776	16.81695095343955
15	15.691495123196667	25.102616607436673	43.49639204237739	15.709496226989279
16	17.321291334196424	23.782191802018808	41.6355214904295	17.26099537335527
17	18.131289425769946	23.48076357690701	35.594320110172944	22.7936268871501
18	19.85557853690742	24.609778364633247	37.51569293933783	18.018950159121506
19	22.3784667598529	26.339225385166888	33.6376671807383	17.644640674241916
20	23.675784131175952	24.69168596584432	33.59697127560257	18.035558627377153
21	21.054534576045636	26.795339313069626	34.38938089613518	17.76074521474956
22	20.38199476987987	24.911619222496736	33.457914038282006	21.248471969341388
23	18.168168477952516	27.996461674154233	33.37858539176901	20.456784456124243
24	17.75295677156135	25.51117461070679	38.26240348262042	18.473465135111436
25	17.546176183869157	26.84475208508487	36.57014498883313	19.038926742212848
26	17.52301717068038	29.601087287300707	34.491765397649026	18.384130144369884
27	18.43287238816363	28.337502643428564	34.64041634644646	18.589208621961347
28	17.133182378518338	27.0385863201927	38.42240183208942	17.405829469199542
29	18.207832801209015	25.337198326774192	36.99840620599659	19.456562666020208
30	19.437426822160443	27.081242230898972	34.84322534390361	18.638105603036976
31	22.437060610593313	26.564213393027536	31.884751672452122	19.113974323927028
32	23.350268469184073	26.930734434718918	32.003074114000114	17.715922982096895
33	22.86795236154882	28.09193457707722	30.57108372834323	18.469029333030736
34	20.418822242968478	28.641097190486754	31.488263177169028	19.45181738937574
35	20.538846794617204	28.165898997818207	31.648364684825946	19.646889522738643
36	22.93082727708805	29.02639302238017	30.003662115671276	18.039117584860502
37	20.869675103287136	30.384264250014187	30.938172140068186	17.807888506630494
38	20.73201050150353	30.94039004110854	29.351805526184126	18.975793931203803
39	21.30350686259845	29.963378843287238	29.7792414778442	18.95387281627011
40	22.49044497284361	27.813097995120618	29.7918783558648	19.904578676170974
41	20.039922218726307	27.025227334856638	30.271048138768393	22.663802307648666
42	21.970011914770705	27.774981044682967	28.94396963022948	21.31103741031685
43	21.34951541441223	28.2640540136272	29.793528886871574	20.592901685089
44	20.75810952304812	30.17660681772464	29.78527623183771	19.28000742738953
45	19.13305858869283	30.88473619872393	29.369136101755238	20.613069110828
46	21.405839785018337	30.10057923322519	29.191807176715134	19.301773805041343
47	20.98939018037209	28.144235778354314	30.087168668795165	20.779205372478426
48	21.59998349468993	26.406432944598894	30.578253222403895	21.415330338307278
49	21.08398623869773	26.08715835297637	31.180387565312028	21.64846784301387
50	19.791414144019146	28.40945547950505	30.602856450223598	21.196273926252214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1069.0
1	1544.0
2	2019.0
3	21845.5
4	41672.0
5	31458.0
6	21244.0
7	21592.5
8	21941.0
9	22355.0
10	22769.0
11	22952.5
12	23136.0
13	22374.5
14	21613.0
15	20770.5
16	19928.0
17	18813.0
18	17698.0
19	16895.5
20	16093.0
21	16229.0
22	16365.0
23	17141.5
24	17918.0
25	19674.5
26	21431.0
27	24865.5
28	28300.0
29	32831.5
30	37363.0
31	43563.0
32	49763.0
33	56309.0
34	62855.0
35	69310.5
36	75766.0
37	82818.0
38	89870.0
39	101162.5
40	112455.0
41	125360.0
42	138265.0
43	147567.0
44	156869.0
45	162893.5
46	168918.0
47	168465.0
48	168012.0
49	160579.0
50	153146.0
51	135550.5
52	117955.0
53	102621.5
54	87288.0
55	77170.5
56	67053.0
57	59358.0
58	51663.0
59	45951.5
60	40240.0
61	34025.0
62	27810.0
63	22769.0
64	17728.0
65	13472.5
66	9217.0
67	7181.5
68	5146.0
69	4288.5
70	3431.0
71	2782.0
72	2133.0
73	1679.0
74	1225.0
75	973.0
76	721.0
77	563.5
78	406.0
79	274.5
80	143.0
81	116.5
82	90.0
83	54.0
84	18.0
85	16.5
86	15.0
87	9.0
88	3.0
89	3.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1938770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.46807767858074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.72146858089339	29.88580803886911
2	7.535138550812361	5.94794867284661
3	3.5114680725539706	4.157726839602489
4	2.358717501739534	3.7237618232193523
5	1.6065660313003043	3.170403645956479
6	1.3094293315897851	3.1008395144278618
7	1.0791943136405755	2.9815607500734194
8	0.8555170308832917	2.7012490104200406
9	0.7592045083853899	2.696790825979494
>10	5.158215967993315	36.66088246595763
>50	0.08928991614419002	2.1918377829360507
>100	0.013973744373452774	1.0246869338866367
>500	0.0011178151941210405	0.2854435694197797
>1k	4.1918069779539027E-4	0.21215251382494063
>5k	1.3972689926513006E-4	0.47978343391655404
>10k+	1.3972689926513006E-4	0.7791241786635719
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14128	0.7287094394899859	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8700	0.4487381174662286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06870335315689845	0.0
2	0.0	0.0	0.0	0.21255744621589978	0.0
3	0.0	0.0	0.0	0.32654724387111417	0.0
4	0.0	0.0	0.0	0.42645594887480204	0.0
5	0.0	0.0	0.0	0.6398902396880496	0.0
6	0.0	0.0	0.0	1.0065144395673546	0.0
7	0.0	0.0	0.0	1.2749836236376673	0.0
8	0.0	0.0	0.0	1.9856403802410807	0.0
9	0.0	0.0	0.0	2.708366644831517	0.0
10	0.0	0.0	0.0	3.7550611985949853	0.0
11	0.0	0.0	0.0	4.318253325562083	0.0
12	0.0	0.0	0.0	4.882631771690298	0.0
13	0.0	0.0	0.0	5.20154530965509	0.0
14	0.0	0.0	0.0	5.350763628486102	0.0
15	0.0	0.0	0.0	5.4864682246991645	0.0
16	0.0	0.0	0.0	5.718625726620486	0.0
17	0.0	0.0	0.0	5.985495958777988	0.0
18	0.0	0.0	0.0	6.410714009397711	0.0
19	0.0	0.0	0.0	6.590054519102318	0.0
20	0.0	0.0	0.0	6.806222501895531	0.0
21	0.0	0.0	0.0	6.997116728647544	0.0
22	0.0	0.0	0.0	7.203484683588048	0.0
23	0.0	0.0	0.0	7.434043233596559	0.0
24	0.0	0.0	0.0	7.615859539811324	0.0
25	0.0	0.0	0.0	7.770648400790192	0.0
26	0.0	0.0	0.0	7.914450914755231	0.0
27	0.0	0.0	0.0	8.116486225802957	0.0
28	0.0	0.0	0.0	8.277000366211567	0.0
29	0.0	0.0	0.0	8.447778746318543	0.0
30	0.0	0.0	0.0	8.670548853138845	0.0
31	0.0	0.0	0.0	8.851952526601918	0.0
32	0.0	0.0	0.0	9.023091960366624	0.0
33	5.157909396163547E-5	0.0	0.0	9.191291385775518	0.0
34	5.157909396163547E-5	0.0	0.0	9.357633963801792	0.0
35	5.157909396163547E-5	0.0	0.0	9.539656586392404	0.0
36	5.157909396163547E-5	0.0	0.0	9.718945517003048	0.0
37	1.0315818792327094E-4	0.0	0.0	9.898595501271425	0.0
38	1.0315818792327094E-4	0.0	0.0	10.084434976815198	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCGA	20	7.8578206E-4	44.000004	2
TGCGCGA	50	2.7284841E-11	44.0	2
ACGTACG	25	4.4439745E-5	44.0	1
CGTTTTT	20460	0.0	43.33333	1
GAGCGAT	1125	0.0	41.65333	7
CGACGGT	135	0.0	40.74074	28
GTTAGCG	55	7.8216544E-11	40.0	1
GTTTTTA	8560	0.0	39.682243	2
ACGTGCG	50	1.3496901E-9	39.600002	1
ATTACGA	75	0.0	38.13333	2
TATAGCG	145	0.0	37.931034	1
GCGCGAC	325	0.0	37.907692	9
AGACCGA	335	0.0	37.432835	8
AATGCGG	160	0.0	37.125004	2
GCGAGGT	285	0.0	37.052628	5
CGCGAGA	155	0.0	36.903225	4
CGAGGAT	215	0.0	36.837208	6
CGGTCTA	150	0.0	36.666668	31
GCGCGAG	210	0.0	36.666664	3
AACGCGA	30	1.3014252E-4	36.666664	2
>>END_MODULE