FastQCFastQC Report
Thu 26 May 2016
SRR1546779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546779_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1756911
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102680.5844348404671608No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62740.35710403088147324No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA27380.15584170171397413No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGA254.4438035E-544.02
ACCGCGA254.4438035E-544.02
TGCGCGA254.4438035E-544.02
TCGACGA254.4438035E-544.02
GTACGCG254.4438035E-544.01
CGTTTTT173900.043.430711
GAGCGAT12300.042.032527
CACGACG2850.040.1403526
GTTTTTA76200.039.9580042
TTCGCGA501.3496901E-939.6000022
TTAGCGA1500.039.62
CGACGGT2900.039.44827728
GTAAGCG452.3521352E-839.111111
TAATGCG850.038.823531
AGCGATA2400.038.58
GCGAAGA1950.038.3589742
ACGAGAT2500.037.845
CGCGTCA357.2924067E-637.71428739
CGGTCTA3050.037.50819831
CGAGAAT2300.037.3043486
Produced by FastQC (version 0.11.5)