##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546779_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1756911
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46407814624645	31.0	31.0	33.0	30.0	34.0
2	31.85684932247564	31.0	31.0	34.0	30.0	34.0
3	31.923170837908124	31.0	31.0	34.0	30.0	34.0
4	35.658766437229886	37.0	35.0	37.0	33.0	37.0
5	35.553235764361425	37.0	35.0	37.0	33.0	37.0
6	35.60253137466838	37.0	35.0	37.0	33.0	37.0
7	36.0190151920046	37.0	35.0	37.0	35.0	37.0
8	22.17535378855275	35.0	0.0	37.0	0.0	37.0
9	30.000114405339826	37.0	18.0	39.0	17.0	39.0
10	34.305766769062295	35.0	32.0	39.0	27.0	39.0
11	35.95056095613267	37.0	35.0	39.0	32.0	39.0
12	36.423160308063416	37.0	35.0	39.0	32.0	39.0
13	36.55179744449207	39.0	35.0	39.0	32.0	39.0
14	37.71346812672924	39.0	36.0	41.0	33.0	41.0
15	37.97153754515738	40.0	36.0	41.0	33.0	41.0
16	38.00508619958552	40.0	36.0	41.0	33.0	41.0
17	37.96208174460744	40.0	36.0	41.0	33.0	41.0
18	37.87868366695866	39.0	36.0	41.0	33.0	41.0
19	37.83027711705374	39.0	36.0	41.0	33.0	41.0
20	37.65882677039418	39.0	35.0	41.0	33.0	41.0
21	37.58394306826015	39.0	35.0	41.0	33.0	41.0
22	37.54925320633772	39.0	35.0	41.0	33.0	41.0
23	37.38483622676391	39.0	35.0	41.0	33.0	41.0
24	37.19499337188964	39.0	35.0	41.0	33.0	41.0
25	37.059960920046606	38.0	35.0	41.0	32.0	41.0
26	36.99153457403363	39.0	35.0	41.0	32.0	41.0
27	36.8742594246379	38.0	35.0	40.0	32.0	41.0
28	36.88267760859827	38.0	35.0	40.0	32.0	41.0
29	36.786718849161964	38.0	35.0	40.0	32.0	41.0
30	36.642443470386375	38.0	35.0	40.0	31.0	41.0
31	36.444113560675525	38.0	35.0	40.0	31.0	41.0
32	35.99002112229931	38.0	35.0	40.0	30.0	41.0
33	35.64310713519353	38.0	35.0	40.0	27.0	41.0
34	35.33990623315581	38.0	35.0	41.0	24.0	41.0
35	35.02140404380188	38.0	35.0	41.0	23.0	41.0
36	34.781859752713714	38.0	35.0	40.0	21.0	41.0
37	34.668539271482736	38.0	34.0	40.0	20.0	41.0
38	34.50825625202415	38.0	34.0	40.0	18.0	41.0
39	34.316159441201066	38.0	34.0	40.0	18.0	41.0
40	34.03824098090342	38.0	33.0	40.0	15.0	41.0
41	33.92777721808333	38.0	34.0	40.0	15.0	41.0
42	33.38030554763446	38.0	33.0	40.0	12.0	41.0
43	33.17854404690961	38.0	33.0	40.0	10.0	41.0
44	32.679253530770765	37.0	31.0	40.0	10.0	41.0
45	32.36243440902812	37.0	31.0	40.0	9.0	41.0
46	32.2667477180119	37.0	30.0	40.0	9.0	41.0
47	32.076978856641006	37.0	30.0	40.0	9.0	41.0
48	32.0762269688106	37.0	30.0	40.0	9.0	41.0
49	31.951159165148376	36.0	30.0	40.0	9.0	41.0
50	31.484298293994403	36.0	29.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	1.0
13	4.0
14	6.0
15	22.0
16	80.0
17	247.0
18	706.0
19	1538.0
20	2995.0
21	5328.0
22	8694.0
23	13793.0
24	20687.0
25	32104.0
26	43166.0
27	46358.0
28	44903.0
29	45093.0
30	51111.0
31	60684.0
32	75360.0
33	93861.0
34	132997.0
35	195402.0
36	154944.0
37	217291.0
38	255428.0
39	254102.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.198747119233698	24.169522531306367	28.825535271849283	17.80619507761065
2	35.346241215405904	25.03120533709448	27.609992765712093	12.012560681787523
3	25.427241334364687	23.743376869972355	37.79394630689887	13.035435488764085
4	22.883800033126324	26.100980641591974	37.30923194174321	13.705987383538496
5	20.771854692696444	30.608721784996508	35.80363490239403	12.815788619913018
6	18.307131095428282	38.363525528612435	32.86028717447839	10.469056201480894
7	81.91610161243229	3.2553726398206853	12.98899033587928	1.8395354118677611
8	50.96604210458015	39.54878761644728	7.5262207362808935	1.9589495426916903
9	76.64076324867908	5.212899230524483	14.863928793205803	3.2824087275906404
10	38.619258459876455	28.584714877418378	21.17546079454224	11.62056586816293
11	31.924041684524713	23.70262352503912	30.9317888043276	13.441545986108574
12	28.937720806574724	22.05330833491281	32.983970161265994	16.025000697246476
13	20.102213487194287	32.10999305030249	34.00900785526416	13.778785607239069
14	16.504023254450566	34.77415759819365	33.14533291669299	15.576486230662795
15	14.964901466266648	24.40362659235442	46.02913864162726	14.602333299751665
16	16.043100646532466	22.75226235136555	43.7348277744291	17.469809227672886
17	16.946959749241707	22.67291854852067	34.112257251505625	26.26786445073199
18	19.80401966861156	24.38655116849971	37.31350079770689	18.495928365181843
19	23.975716470555426	26.854405260141235	31.48378033947081	17.68609792983253
20	24.798410391875287	24.896821751357923	31.394703545028747	18.910064311738044
21	19.975798432589926	28.472130916136333	33.26776370573125	18.28430694554249
22	20.237621598362125	25.39508261943832	31.435343053802956	22.931952728396602
23	17.624000305080905	29.925192568092523	31.055187200717622	21.39561992610895
24	17.597305725788047	25.55519317711597	38.44008034556104	18.40742075153494
25	16.478410118668503	27.55239166924221	36.56667867638144	19.40251953570784
26	16.23565450953406	31.375977496868085	33.30009317489617	19.088274818701688
27	17.796063659456856	30.292940279843428	33.427475836852295	18.483520223847425
28	16.529579472153113	27.58375353105536	38.96384051326447	16.922826483527054
29	16.87433227977968	24.824421954213957	38.248095663354604	20.053150102651756
30	18.013319968968265	28.232619637534285	35.17281182712158	18.581248566375873
31	23.027518183903453	25.597141801719044	31.80428604522369	19.571053969153816
32	24.0307562534471	25.878203278367547	32.675474170290926	17.41556629789443
33	22.666999068251037	26.48449466136873	31.818287892784554	19.030218377595677
34	18.333541084323564	26.36394216895449	34.471182660931596	20.831334085790346
35	20.510259199242306	25.534133487695165	33.36976090422338	20.58584640883915
36	25.088521843166784	24.959545474984218	31.254969659817718	18.696963022031284
37	21.620218667877882	28.6272326828166	31.518272695657323	18.234275953648194
38	21.30250194802127	29.58339950060077	29.09572539531029	20.018373156067668
39	21.892400924121937	28.581584383045016	28.69382683584997	20.83218785698308
40	22.941458047675724	25.82680625256487	28.887348306203332	22.344387393556076
41	18.572198591732878	25.23286609281859	29.284579583143373	26.910355732305163
42	21.02115588097519	26.796519573273773	28.346171206168098	23.83615333958294
43	20.86776165668039	26.968526009570205	28.988377897343693	23.175334436405713
44	19.96162583079052	29.411620736622403	29.939479006050963	20.68727442653612
45	18.470030639002204	31.814474381457003	27.715860393611287	21.99963458592951
46	21.334319154470545	29.668662783715284	28.682044793390215	20.314973268423955
47	21.11541222065318	26.656387261506133	29.71727082362169	22.510929694219
48	21.958141305962567	25.09597811158334	30.68533352002463	22.260547062429456
49	20.80611937656489	24.23594593010118	31.283371781496044	23.674562911837878
50	19.354366840437564	28.870215964269107	29.38834124210048	22.38707595319285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1113.0
1	1386.0
2	1659.0
3	15000.5
4	28342.0
5	21880.5
6	15419.0
7	16101.0
8	16783.0
9	17860.0
10	18937.0
11	19432.5
12	19928.0
13	19689.0
14	19450.0
15	18784.5
16	18119.0
17	17364.5
18	16610.0
19	15988.0
20	15366.0
21	14935.5
22	14505.0
23	14961.5
24	15418.0
25	16335.0
26	17252.0
27	19431.5
28	21611.0
29	24902.5
30	28194.0
31	32158.0
32	36122.0
33	41846.0
34	47570.0
35	53828.5
36	60087.0
37	67339.5
38	74592.0
39	88509.5
40	102427.0
41	119778.0
42	137129.0
43	148378.5
44	159628.0
45	165147.5
46	170667.0
47	167887.0
48	165107.0
49	157550.0
50	149993.0
51	134815.0
52	119637.0
53	102065.5
54	84494.0
55	71675.0
56	58856.0
57	50631.5
58	42407.0
59	36493.0
60	30579.0
61	25216.5
62	19854.0
63	16045.0
64	12236.0
65	9537.0
66	6838.0
67	5405.0
68	3972.0
69	3240.5
70	2509.0
71	2055.0
72	1601.0
73	1251.0
74	901.0
75	691.0
76	481.0
77	388.5
78	296.0
79	206.0
80	116.0
81	85.5
82	55.0
83	39.5
84	24.0
85	18.5
86	13.0
87	12.5
88	12.0
89	6.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1756911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.77044382616894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7580570491292	35.34815999579457
2	7.023267207052348	6.148230455249185
3	2.8361786633750365	3.724223965987078
4	1.6645739903529793	2.914365693569878
5	1.1055070237733629	2.4194266541753597
6	0.8296665062934458	2.1788922724902666
7	0.6603318774949851	2.0232113545366044
8	0.550488625343312	1.9276105162027517
9	0.45559855661880844	1.7947575926790484
>10	3.8882546615187694	33.44358671597133
>50	0.21022520551521726	5.777086455071262
>100	0.01731379827860036	1.117935768816224
>500	1.3420881348511626E-4	0.049932224318830064
>1k	1.3420881348511626E-4	0.16084050609994907
>5k	1.3420881348511626E-4	0.3685585592662821
>10k+	1.3420881348511626E-4	0.6031812697714671
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10268	0.5844348404671608	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6274	0.35710403088147324	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2738	0.15584170171397413	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07205828866687043	0.0
2	0.0	0.0	0.0	0.2609124764999479	0.0
3	0.0	0.0	0.0	0.4412858704851868	0.0
4	0.0	0.0	0.0	0.6106171570443807	0.0
5	0.0	0.0	0.0	0.9726161427642037	0.0
6	0.0	0.0	0.0	1.54891169786062	0.0
7	0.0	0.0	0.0	1.9359546385673492	0.0
8	0.0	0.0	0.0	2.929687388831876	0.0
9	0.0	0.0	0.0	3.899969890335936	0.0
10	0.0	0.0	0.0	5.302032943046062	0.0
11	0.0	0.0	0.0	6.107309932034121	0.0
12	0.0	0.0	0.0	6.872630429202162	0.0
13	0.0	0.0	0.0	7.293767299538793	0.0
14	0.0	0.0	0.0	7.477328105976911	0.0
15	0.0	0.0	0.0	7.666580720366598	0.0
16	0.0	0.0	0.0	8.00029142056712	0.0
17	0.0	0.0	0.0	8.387789705909976	0.0
18	0.0	0.0	0.0	9.003870998587862	0.0
19	0.0	0.0	0.0	9.249870938254698	0.0
20	0.0	0.0	0.0	9.563261884068117	0.0
21	0.0	0.0	0.0	9.86919656146498	0.0
22	0.0	0.0	0.0	10.178660159791816	0.0
23	0.0	0.0	0.0	10.538211668092465	0.0
24	0.0	0.0	0.0	10.807149593804125	0.0
25	0.0	0.0	0.0	11.04034296557993	0.0
26	0.0	0.0	0.0	11.268242955960774	0.0
27	0.0	0.0	0.0	11.53501799465084	0.0
28	0.0	0.0	0.0	11.779993408886392	0.0
29	0.0	0.0	0.0	12.039653687636994	0.0
30	0.0	0.0	0.0	12.381219082810683	0.0
31	0.0	0.0	0.0	12.660231508596622	0.0
32	0.0	0.0	0.0	12.927461891922812	0.0
33	0.0	0.0	0.0	13.17898288530267	0.0
34	0.0	0.0	0.0	13.417981901189076	0.0
35	0.0	0.0	0.0	13.693294651806495	0.0
36	0.0	0.0	0.0	13.953467193272738	0.0
37	0.0	0.0	0.0	14.23561011343204	0.0
38	0.0	0.0	0.0	14.512971914912024	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGA	25	4.4438035E-5	44.0	2
ACCGCGA	25	4.4438035E-5	44.0	2
TGCGCGA	25	4.4438035E-5	44.0	2
TCGACGA	25	4.4438035E-5	44.0	2
GTACGCG	25	4.4438035E-5	44.0	1
CGTTTTT	17390	0.0	43.43071	1
GAGCGAT	1230	0.0	42.03252	7
CACGACG	285	0.0	40.14035	26
GTTTTTA	7620	0.0	39.958004	2
TTCGCGA	50	1.3496901E-9	39.600002	2
TTAGCGA	150	0.0	39.6	2
CGACGGT	290	0.0	39.448277	28
GTAAGCG	45	2.3521352E-8	39.11111	1
TAATGCG	85	0.0	38.82353	1
AGCGATA	240	0.0	38.5	8
GCGAAGA	195	0.0	38.358974	2
ACGAGAT	250	0.0	37.84	5
CGCGTCA	35	7.2924067E-6	37.714287	39
CGGTCTA	305	0.0	37.508198	31
CGAGAAT	230	0.0	37.304348	6
>>END_MODULE