FastQCFastQC Report
Thu 26 May 2016
SRR1546778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546778_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2534097
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80840.3190090987045879No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48810.192612989952634No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45930.18124799484786888No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC27850.10990108113462112No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA25490.10058809903488303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAT207.858279E-444.03
CGCTAAC254.4443637E-544.041
CGCACGA254.4443637E-544.02
CGTTTTT105050.042.722511
CGACGGT16200.042.09876628
CGGTCTA16650.041.4894931
TCACGAC17000.041.0235325
ACGGTCT16750.040.8477630
CACGACG17000.040.50588226
GACGGTC16900.040.48520729
TCTCACG17500.040.10285623
GAGCGAT16350.040.097867
GTTAGCG1100.040.01
GCGTAAG1900.039.368421
CTCACGA17900.039.32961324
ATTCGAT1300.038.9230814
GTATGCG1300.038.923081
ACGACGG17750.038.9183127
AGCGATC2100.038.76198
CGAGGTT2000.038.56
Produced by FastQC (version 0.11.5)