FastQCFastQC Report
Thu 26 May 2016
SRR1546777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546777_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2568941
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA81990.31915875062915033No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49760.19369849288091864No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29750.11580647434098332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGT408.3200575E-944.031
CGAACGC207.8583E-444.040
TGCGCAA254.4443805E-544.013
ACCGTTC207.8583E-444.012
TTCGCAC254.4443805E-544.01
ACGGTCC254.4443805E-544.033
CGACGGT11400.043.42105528
GACGGTC11550.043.23809429
CGGTCTA11500.043.04347631
TCACGAC11750.042.5021325
ACGGTCT12000.041.61666530
CGTTTTT67000.041.6029851
GGGCGTA700.040.8571437
CTCACGA12300.040.78048724
CACGACG12200.040.57377226
ACGACGG12150.040.5596727
ACGTTAG1150.040.173911
GGTCTAA12450.039.9357432
ACGCTTA501.3496901E-939.60000244
TCGTAGA1450.039.4482772
Produced by FastQC (version 0.11.5)