##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546777_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2568941
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.407292732686347	31.0	31.0	33.0	30.0	34.0
2	31.790808741812288	31.0	31.0	34.0	30.0	34.0
3	31.946334695892197	31.0	31.0	34.0	30.0	34.0
4	35.64184930677661	37.0	35.0	37.0	33.0	37.0
5	35.51743072339925	37.0	35.0	37.0	33.0	37.0
6	35.57228912614186	37.0	35.0	37.0	33.0	37.0
7	35.940585245048446	37.0	35.0	37.0	35.0	37.0
8	22.144263336526606	35.0	0.0	37.0	0.0	37.0
9	29.96301588864828	37.0	18.0	39.0	17.0	39.0
10	34.28909344356293	35.0	32.0	39.0	27.0	39.0
11	35.89976531185418	37.0	35.0	39.0	32.0	39.0
12	36.108847964978565	37.0	35.0	39.0	32.0	39.0
13	36.100920963151744	37.0	35.0	39.0	32.0	39.0
14	37.176142620636284	39.0	35.0	41.0	32.0	41.0
15	37.507168907343534	39.0	35.0	41.0	33.0	41.0
16	37.625713864195404	39.0	35.0	41.0	33.0	41.0
17	37.59173877484925	39.0	35.0	41.0	33.0	41.0
18	37.57616153893764	39.0	36.0	41.0	33.0	41.0
19	37.58555451448671	39.0	36.0	41.0	33.0	41.0
20	37.47135453869902	39.0	35.0	41.0	33.0	41.0
21	37.348741757790464	39.0	35.0	41.0	32.0	41.0
22	37.30603466564627	39.0	35.0	41.0	32.0	41.0
23	37.213697005886864	39.0	35.0	41.0	32.0	41.0
24	37.126703571627374	39.0	35.0	41.0	32.0	41.0
25	36.965350313611715	38.0	35.0	41.0	32.0	41.0
26	36.90108686809078	38.0	35.0	41.0	32.0	41.0
27	36.77710581909044	38.0	35.0	40.0	31.0	41.0
28	36.83152824451788	38.0	35.0	41.0	32.0	41.0
29	36.750298274658704	38.0	35.0	40.0	31.0	41.0
30	36.68177509720932	38.0	35.0	40.0	31.0	41.0
31	36.61179879179787	38.0	35.0	40.0	31.0	41.0
32	36.37360531051511	38.0	35.0	40.0	31.0	41.0
33	36.27594405632515	38.0	35.0	40.0	30.0	41.0
34	36.234808039577395	38.0	35.0	40.0	30.0	41.0
35	36.124640464689534	38.0	35.0	40.0	30.0	41.0
36	35.97202076653376	38.0	35.0	40.0	30.0	41.0
37	35.93595648946395	38.0	35.0	40.0	30.0	41.0
38	35.86421330812969	38.0	35.0	40.0	30.0	41.0
39	35.805655715720995	38.0	35.0	40.0	29.0	41.0
40	35.71771247373918	38.0	35.0	40.0	29.0	41.0
41	35.74012949304792	38.0	35.0	40.0	29.0	41.0
42	35.503798257725656	38.0	35.0	40.0	28.0	41.0
43	35.519023208395986	38.0	35.0	40.0	29.0	41.0
44	35.31988239511924	38.0	34.0	40.0	28.0	41.0
45	35.092962820088125	37.0	34.0	40.0	27.0	41.0
46	35.16725530091972	37.0	34.0	40.0	27.0	41.0
47	35.03612500248157	37.0	34.0	40.0	27.0	41.0
48	35.078244303781204	37.0	34.0	40.0	27.0	41.0
49	35.01664615886468	37.0	34.0	40.0	27.0	41.0
50	34.5107011021273	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	0.0
13	2.0
14	22.0
15	38.0
16	133.0
17	341.0
18	787.0
19	1742.0
20	3265.0
21	5383.0
22	8401.0
23	12217.0
24	17240.0
25	23897.0
26	30830.0
27	37430.0
28	42427.0
29	50183.0
30	62424.0
31	79689.0
32	103247.0
33	137039.0
34	219211.0
35	359188.0
36	242797.0
37	348919.0
38	414296.0
39	367789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.33525370960252	24.473820146122467	29.056564553253654	13.13436159102136
2	29.30927568986598	26.341087631051085	30.897595546180312	13.452041132902623
3	28.72627281046937	25.974049228845658	31.78955842115487	13.510119539530102
4	25.266987447356716	28.741454163408193	30.855632729595577	15.135925659639518
5	22.57809735607007	33.1479002437191	30.21895014326915	14.055052256941675
6	20.26807933697193	42.01221437160293	26.72435840293724	10.995347888487903
7	87.45409100481483	4.706842235769526	5.89815024946077	1.9409165099548802
8	54.59374115637533	39.989902453968384	3.247096760883181	2.1692596287731014
9	83.33441678886359	5.11751729603755	8.210231375496752	3.3378345396021163
10	49.71406505637926	25.01735150787815	13.23989145721914	12.028691978523447
11	43.790846111296446	20.96646049870355	21.314814158830426	13.927879231169577
12	39.81270881659018	21.08172200140058	23.66364194428755	15.441927237721693
13	22.13484077680258	40.04572312092804	23.10800442672681	14.711431675542569
14	15.816673096034513	41.71952567225172	25.805108019218814	16.658693212494953
15	13.369945047395015	23.117774989772048	47.1975417107672	16.314738252065737
16	14.719917662569907	18.487851608892537	44.79663020676614	21.995600521771422
17	15.051026862820127	20.046937629163146	28.723781511525566	36.17825399649116
18	20.341961921274173	23.346001328952283	33.83943033335527	22.472606416418284
19	27.586231057856136	25.924145396877545	24.7200305495533	21.76959299571302
20	29.283778802237965	23.765395935523625	23.906971783314603	23.043853478923808
21	21.16140464105637	30.116339768021145	26.336727857899422	22.385527733023064
22	22.553729338275964	25.657537483344306	23.427007471171972	28.361725707207757
23	18.494819460625994	30.974047282518363	23.75274480807461	26.778388448781033
24	19.26291806623819	24.517145391817095	37.0703725776497	19.149563964295016
25	16.630198980825174	26.70832066598649	33.88984021042133	22.771640142766998
26	17.043715678950978	33.3136494765742	26.78111330700082	22.861521537474
27	18.300186730641148	32.390195025888104	27.267967617784915	22.04165062568584
28	16.03606310927343	28.49037794172774	36.686712540303574	18.786846408695258
29	16.173785229010708	23.892335402019743	35.426738099473674	24.507141269495875
30	19.779473331617968	29.934124606209327	29.90995122114521	20.37645084102749
31	27.982892561565254	26.55086278742875	22.816172111387534	22.650072539618467
32	30.19816336770677	26.542104314579433	23.49680276814454	19.762929549569257
33	26.652461072480836	27.34083811189124	23.329924665455533	22.676776150172387
34	19.63493906633122	26.51220872725376	27.505069209452454	26.34778299696256
35	19.872352070366738	26.138630665320843	28.615371080924007	25.37364618338841
36	28.57994792406677	24.868262836709757	25.978486855089315	20.573302384134163
37	21.244123551299932	31.358914042790392	27.498724182454946	19.898238223454722
38	19.867603031755106	32.69249858210056	24.967564455548025	22.472333930596307
39	20.086681632626053	32.35266983554702	26.254670698937808	21.305977832889116
40	23.248568184321865	26.25439821311583	26.02951955689134	24.46751404567096
41	17.80216050115592	24.4708617286267	27.15064300815005	30.576334762067326
42	21.90497952269048	26.689013099171994	24.146214335011976	27.25979304312555
43	21.343970141782158	26.779634098252934	26.11161564239895	25.764780117565955
44	20.6537246281639	29.767090797336333	27.537222536445956	22.041962038053814
45	17.284164953574255	35.089050312949965	24.085449996710707	23.54133473676507
46	22.344032034990295	30.76458353850867	26.31547396378508	20.57591046271596
47	21.058093587980416	27.063642177846823	27.035926477096982	24.842337757075775
48	21.950796067328913	24.43372580374559	29.231811863332013	24.383666265593487
49	21.07280782236727	22.799589402792826	31.176387468610606	24.951215306229297
50	19.740118593615033	29.723181653451753	27.50467994399249	23.032019808940728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1524.0
1	2060.0
2	2596.0
3	8018.5
4	13441.0
5	10219.0
6	6997.0
7	7682.5
8	8368.0
9	9419.0
10	10470.0
11	10939.5
12	11409.0
13	11218.5
14	11028.0
15	10539.0
16	10050.0
17	9414.0
18	8778.0
19	8721.5
20	8665.0
21	8891.5
22	9118.0
23	9708.0
24	10298.0
25	12402.0
26	14506.0
27	17954.5
28	21403.0
29	26518.5
30	31634.0
31	36502.5
32	41371.0
33	47468.0
34	53565.0
35	61287.5
36	69010.0
37	78055.5
38	87101.0
39	115278.0
40	143455.0
41	180615.0
42	217775.0
43	238072.0
44	258369.0
45	265392.5
46	272416.0
47	265136.0
48	257856.0
49	247961.0
50	238066.0
51	212439.0
52	186812.0
53	159079.5
54	131347.0
55	116486.5
56	101626.0
57	93663.0
58	85700.0
59	78055.0
60	70410.0
61	62716.0
62	55022.0
63	48263.0
64	41504.0
65	34398.5
66	27293.0
67	22541.0
68	17789.0
69	14918.5
70	12048.0
71	10330.5
72	8613.0
73	7113.5
74	5614.0
75	4192.0
76	2770.0
77	2214.5
78	1659.0
79	1265.0
80	871.0
81	573.5
82	276.0
83	218.5
84	161.0
85	128.0
86	95.0
87	62.5
88	30.0
89	23.5
90	17.0
91	12.0
92	7.0
93	5.5
94	4.0
95	3.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2568941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.017026123207142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.41920620109107	18.049769176916808
2	7.354601430081788	3.3856210648393814
3	2.557648951285147	1.7660841817717075
4	1.3545174918990106	1.2470785798152217
5	0.8909207962644917	1.0253173620664158
6	0.6743685455210815	0.9313175057356762
7	0.5097476944792093	0.8213013200050803
8	0.4413836610390286	0.8127471405193702
9	0.3523638414662718	0.7299330969512517
>10	5.3516810910348145	30.92654768280547
>50	1.7106480016401762	27.16393186417359
>100	0.3789399003423924	11.846260389605916
>500	0.0025906916449950205	0.39105365325682306
>1k	0.0012089894343310097	0.5770993071393007
>5k	1.7271277633300138E-4	0.32593767439795596
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	8199	0.31915875062915033	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4976	0.19369849288091864	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2975	0.11580647434098332	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06021936665731132	0.0
2	0.0	0.0	0.0	0.19443809725486105	0.0
3	0.0	0.0	0.0	0.28688864399766284	0.0
4	0.0	0.0	0.0	0.42788059359868524	0.0
5	0.0	0.0	0.0	0.6973301449897059	0.0
6	0.0	0.0	0.0	1.1236926032945094	0.0
7	0.0	0.0	0.0	1.4050926043065994	0.0
8	0.0	0.0	0.0	2.3750642774590776	0.0
9	0.0	0.0	0.0	3.170138979447173	0.0
10	0.0	0.0	0.0	4.323532537337369	0.0
11	0.0	0.0	0.0	4.988553649149591	0.0
12	0.0	0.0	0.0	5.663929222197006	0.0
13	0.0	0.0	0.0	5.967750913703351	0.0
14	0.0	0.0	0.0	6.0861265400801345	0.0
15	0.0	0.0	0.0	6.223381541265447	0.0
16	0.0	0.0	0.0	6.499409678929956	0.0
17	3.892654599696918E-5	0.0	0.0	6.7975870212667395	0.0
18	3.892654599696918E-5	0.0	0.0	7.265406251058315	0.0
19	3.892654599696918E-5	0.0	0.0	7.441977063700568	0.0
20	3.892654599696918E-5	0.0	0.0	7.680441084478001	0.0
21	7.785309199393836E-5	0.0	0.0	7.915323863023713	0.0
22	7.785309199393836E-5	0.0	0.0	8.13922935559828	0.0
23	7.785309199393836E-5	0.0	0.0	8.415607832176761	0.0
24	7.785309199393836E-5	0.0	0.0	8.606776099567876	0.0
25	7.785309199393836E-5	0.0	0.0	8.761859458819801	0.0
26	7.785309199393836E-5	0.0	0.0	8.905031294996654	0.0
27	7.785309199393836E-5	0.0	0.0	9.06731606525802	0.0
28	7.785309199393836E-5	0.0	0.0	9.219908125566137	0.0
29	7.785309199393836E-5	0.0	0.0	9.388304363549027	0.0
30	7.785309199393836E-5	0.0	0.0	9.626456971958484	0.0
31	7.785309199393836E-5	0.0	0.0	9.818715182637515	0.0
32	7.785309199393836E-5	0.0	0.0	9.990342323938153	0.0
33	7.785309199393836E-5	0.0	0.0	10.155585511695286	0.0
34	1.1677963799090754E-4	0.0	0.0	10.334219431275377	0.0
35	1.1677963799090754E-4	0.0	0.0	10.549016112086653	0.0
36	1.1677963799090754E-4	0.0	0.0	10.724652687624978	0.0
37	1.1677963799090754E-4	0.0	0.0	10.911694741140415	0.0
38	1.1677963799090754E-4	0.0	0.0	11.09363741713025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGGT	40	8.3200575E-9	44.0	31
CGAACGC	20	7.8583E-4	44.0	40
TGCGCAA	25	4.4443805E-5	44.0	13
ACCGTTC	20	7.8583E-4	44.0	12
TTCGCAC	25	4.4443805E-5	44.0	1
ACGGTCC	25	4.4443805E-5	44.0	33
CGACGGT	1140	0.0	43.421055	28
GACGGTC	1155	0.0	43.238094	29
CGGTCTA	1150	0.0	43.043476	31
TCACGAC	1175	0.0	42.50213	25
ACGGTCT	1200	0.0	41.616665	30
CGTTTTT	6700	0.0	41.602985	1
GGGCGTA	70	0.0	40.857143	7
CTCACGA	1230	0.0	40.780487	24
CACGACG	1220	0.0	40.573772	26
ACGACGG	1215	0.0	40.55967	27
ACGTTAG	115	0.0	40.17391	1
GGTCTAA	1245	0.0	39.93574	32
ACGCTTA	50	1.3496901E-9	39.600002	44
TCGTAGA	145	0.0	39.448277	2
>>END_MODULE