FastQCFastQC Report
Thu 26 May 2016
SRR1546776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546776_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1983264
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.23088202074963293No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44210.22291535569646803No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27940.14087887442115624No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA22420.11304596866579537No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.857865E-444.01
CGACGGA207.857865E-444.02
GACACGC254.4440123E-544.09
CGCGAGT600.044.04
CGTTATC254.4440123E-544.017
CCGTAAG254.4440123E-544.01
CGTCGGA207.857865E-444.02
TTACGCG254.4440123E-544.01
CCGGTTA207.857865E-444.025
TGCTACG502.7284841E-1144.01
CTAGCGC207.857865E-444.016
GAATCGT351.4467878E-743.9999969
TATCGTG454.802132E-1043.9999961
CGACGGT9150.043.27868728
CGGTCTA9400.042.361731
TCACGAC9450.042.13756625
CGTTTTT65900.042.030351
GAGCGAT12650.041.9130447
ACGGTCT9450.041.90476230
CGCATGG950.041.684212
Produced by FastQC (version 0.11.5)