##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546776_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1983264
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.482448630136986	31.0	31.0	33.0	30.0	34.0
2	31.87571044500379	31.0	31.0	34.0	30.0	34.0
3	32.015866268938474	33.0	31.0	34.0	30.0	34.0
4	35.70094954579925	37.0	35.0	37.0	33.0	37.0
5	35.57418124868903	37.0	35.0	37.0	33.0	37.0
6	35.640465414589286	37.0	35.0	37.0	33.0	37.0
7	36.00571078787292	37.0	35.0	37.0	35.0	37.0
8	22.001815189505784	35.0	0.0	37.0	0.0	37.0
9	29.921281785985123	37.0	18.0	39.0	17.0	39.0
10	34.28596999693435	35.0	32.0	39.0	27.0	39.0
11	35.932175948335676	37.0	35.0	39.0	32.0	39.0
12	36.25873912903174	37.0	35.0	39.0	32.0	39.0
13	36.30302824031496	38.0	35.0	39.0	32.0	39.0
14	37.397077242364105	39.0	35.0	41.0	33.0	41.0
15	37.70929135001694	39.0	36.0	41.0	33.0	41.0
16	37.79690046307501	40.0	35.0	41.0	33.0	41.0
17	37.774398163834974	40.0	35.0	41.0	33.0	41.0
18	37.741534157832746	39.0	36.0	41.0	33.0	41.0
19	37.74391205608532	39.0	36.0	41.0	33.0	41.0
20	37.638089533213936	39.0	35.0	41.0	33.0	41.0
21	37.53255038159317	39.0	35.0	41.0	33.0	41.0
22	37.50328498878616	39.0	35.0	41.0	33.0	41.0
23	37.39925799086758	39.0	35.0	41.0	33.0	41.0
24	37.31255848943963	39.0	35.0	41.0	33.0	41.0
25	37.196737297707216	39.0	35.0	41.0	32.0	41.0
26	37.119139458992855	39.0	35.0	41.0	32.0	41.0
27	36.98929391145102	39.0	35.0	41.0	32.0	41.0
28	37.02088224260613	39.0	35.0	41.0	32.0	41.0
29	36.94792372573697	39.0	35.0	41.0	32.0	41.0
30	36.891938743404815	39.0	35.0	41.0	32.0	41.0
31	36.79309159042871	39.0	35.0	41.0	31.0	41.0
32	36.57810659599529	39.0	35.0	41.0	31.0	41.0
33	36.470636284428096	39.0	35.0	41.0	31.0	41.0
34	36.3947679179373	39.0	35.0	41.0	30.0	41.0
35	36.2906834390177	39.0	35.0	41.0	30.0	41.0
36	36.12220460816109	38.0	35.0	41.0	30.0	41.0
37	36.07844391871178	38.0	35.0	40.0	30.0	41.0
38	36.00757034867774	38.0	35.0	40.0	30.0	41.0
39	35.973742779579524	38.0	35.0	40.0	30.0	41.0
40	35.88465529551285	38.0	35.0	40.0	29.0	41.0
41	35.920672184842765	38.0	35.0	40.0	30.0	41.0
42	35.686058437000824	38.0	35.0	40.0	29.0	41.0
43	35.73814227455346	38.0	35.0	40.0	29.0	41.0
44	35.53581923536151	38.0	35.0	40.0	28.0	41.0
45	35.33164772818949	38.0	34.0	40.0	27.0	41.0
46	35.409737180728335	38.0	35.0	40.0	28.0	41.0
47	35.31175022589025	38.0	34.0	40.0	27.0	41.0
48	35.35424280378205	38.0	34.0	40.0	28.0	41.0
49	35.29618648853607	38.0	34.0	40.0	27.0	41.0
50	34.79370724220275	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	0.0
13	3.0
14	10.0
15	28.0
16	61.0
17	192.0
18	541.0
19	1194.0
20	2300.0
21	3858.0
22	5981.0
23	9045.0
24	13340.0
25	18735.0
26	24090.0
27	28315.0
28	30487.0
29	35357.0
30	44330.0
31	57132.0
32	75117.0
33	100846.0
34	159472.0
35	262477.0
36	184564.0
37	273659.0
38	333187.0
39	318939.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.775850315439598	24.729536763638123	30.88771842780386	12.606894493118414
2	29.31631895703245	26.403897816932087	31.163828920405955	13.115954305629508
3	27.70387603465802	25.75017748519612	33.21469053035804	13.331255949787824
4	24.757621779047064	28.629622682608062	32.60105563354148	14.011699904803395
5	22.083948480888072	32.59641681591558	31.40519870274457	13.91443600045178
6	19.099323136002067	41.81112549816868	28.068729125320687	11.020822240508576
7	87.82219613727673	3.9564576375106895	6.457839198412314	1.7635070268002646
8	54.646582603223784	40.181034900043564	3.4557174435677753	1.7166650531648837
9	83.86452837342885	5.189727640899043	8.13981396324443	2.805930022427675
10	46.24316278619488	27.35354446004163	14.743876760733821	11.659415993029672
11	40.230952611452636	23.06067170079223	23.297654775158527	13.410720912596608
12	37.04852203236685	22.851521532181295	25.20289784920212	14.897058586249736
13	21.953809477709473	39.951363005631116	24.7303939364603	13.364433580199107
14	15.857445100601836	42.26946084837924	26.281069993707344	15.592024057311582
15	13.541666666666666	24.748243299933847	45.79107975539313	15.919010278006358
16	14.633906529841715	20.778827226229087	44.300254529906255	20.287011714022942
17	16.072192103522273	22.05682148216274	29.05896542265679	32.812020991658194
18	20.458244590735273	24.42211425206125	34.47554132984817	20.64409982735531
19	26.41599908030398	26.85724139600174	25.961697484550722	20.765062039143555
20	28.615807073591814	24.460081965890573	25.93240234280459	20.991708617713023
21	20.88864619132904	30.452375477999905	27.98165045097375	20.67732787969731
22	21.931674250125045	26.40440203623925	25.592255998192876	26.071667715442825
23	17.676315407328524	31.77156445132872	25.76449731351953	24.787622827823224
24	18.433501540894206	25.810986333639896	37.66230819497556	18.093203930490343
25	15.926170192168062	28.705709376058863	34.57366240702196	20.794458024751115
26	16.428624731755328	34.83893218452007	27.667874776126627	21.06456830759798
27	17.23824967326589	34.3439400906788	28.29406473369153	20.12374550236378
28	15.523046856091776	29.579269325717604	36.63087718024428	18.266806637946335
29	15.549367103925649	25.45732691159624	35.89683471287736	23.096471271600755
30	17.65579368152702	31.76500960033561	30.86649079497233	19.712705923165043
31	25.85636607128451	29.00143399970957	24.62506252319409	20.517137405811834
32	26.76622981105894	29.25182930764639	25.298850783355114	18.68309009793956
33	25.14486220694774	29.419230117624277	24.710779805411683	20.725127870016298
34	17.403230230569406	29.786654726753476	28.2551390031786	24.554976039498523
35	18.983655226939025	27.635705584329674	29.132430175710343	24.24820901302096
36	26.52193555673879	26.7924996369621	26.984052551753066	19.701512254546042
37	19.61065193539539	31.8901568323733	28.94102852671152	19.55816270551979
38	18.81262403794956	33.11480468560918	26.52970053406909	21.54287074237217
39	20.108265969633894	31.918746067089405	27.061349371541056	20.91163859173564
40	22.618824321925874	28.038576810752375	25.67222518030882	23.670373687012926
41	17.937601852300048	25.630627087467932	27.49205350371912	28.9397175565129
42	20.939774029075302	28.336469577423884	24.377037046000936	26.34671934749988
43	22.04129152750214	27.75994522161447	26.180074866482727	24.018688384400665
44	20.647931894089744	30.615389580005488	27.49785202575149	21.238826500153284
45	17.291495232102232	35.85594252706649	24.03442002678413	22.818142214047146
46	21.478532358778256	32.4128809881085	26.574878583990834	19.533708069122415
47	20.166805831195443	28.77534206237798	27.210648708391822	23.847203398034754
48	21.349351372283266	26.057952950288012	29.475551414234314	23.11714426319441
49	21.037088355357632	24.23494804524259	30.795950513900316	23.93201308549946
50	19.263597786275554	30.838456201494104	28.042509721348242	21.855436290882103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1099.0
1	1459.0
2	1819.0
3	7222.0
4	12625.0
5	9646.0
6	6667.0
7	7281.0
8	7895.0
9	8542.0
10	9189.0
11	9582.0
12	9975.0
13	9793.5
14	9612.0
15	9208.0
16	8804.0
17	8666.5
18	8529.0
19	8430.5
20	8332.0
21	8617.5
22	8903.0
23	9816.5
24	10730.0
25	12703.5
26	14677.0
27	17849.5
28	21022.0
29	25412.0
30	29802.0
31	35630.5
32	41459.0
33	47430.5
34	53402.0
35	62394.5
36	71387.0
37	81346.0
38	91305.0
39	112673.5
40	134042.0
41	158764.5
42	183487.0
43	197374.5
44	211262.0
45	213914.0
46	216566.0
47	208198.0
48	199830.0
49	186448.5
50	173067.0
51	151917.5
52	130768.0
53	111319.5
54	91871.0
55	79721.0
56	67571.0
57	58185.0
58	48799.0
59	41886.0
60	34973.0
61	29001.0
62	23029.0
63	19350.0
64	15671.0
65	12526.0
66	9381.0
67	7348.5
68	5316.0
69	4637.0
70	3958.0
71	3261.0
72	2564.0
73	2080.0
74	1596.0
75	1297.5
76	999.0
77	846.5
78	694.0
79	476.5
80	259.0
81	199.0
82	139.0
83	101.5
84	64.0
85	59.0
86	54.0
87	56.0
88	58.0
89	32.5
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1983264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.17857791418553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0123831421554	20.160746724440234
2	7.324306172645296	3.834798396158898
3	2.7196125206244695	2.1358676480268652
4	1.5841558380201575	1.6588378813529023
5	1.106323122764651	1.4480983033779733
6	0.7914552478404672	1.2431503722709614
7	0.6601919619489297	1.2098020699939347
8	0.5682057930433095	1.1899855699580686
9	0.46460508912442466	1.0946430472473776
>10	6.512879368501371	40.25253000535309
>50	1.0997749692108703	19.36805480724611
>100	0.1533783291884331	5.3117774297181075
>500	0.0017540003135937726	0.3216247136914248
>1k	9.74444618663207E-4	0.770083031164081
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4579	0.23088202074963293	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4421	0.22291535569646803	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2794	0.14087887442115624	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	2242	0.11304596866579537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05743057908578989	0.0
2	0.0	0.0	0.0	0.1973514368233377	0.0
3	0.0	0.0	0.0	0.31019571776626814	0.0
4	0.0	0.0	0.0	0.44048598673701533	0.0
5	0.0	0.0	0.0	0.6943604078932507	0.0
6	5.042193071623344E-5	0.0	0.0	1.0996014650596189	0.0
7	5.042193071623344E-5	0.0	0.0	1.3617955047840329	0.0
8	5.042193071623344E-5	0.0	0.0	2.1226120173612792	0.0
9	1.0084386143246689E-4	0.0	0.0	2.7743154718685963	0.0
10	1.0084386143246689E-4	0.0	0.0	3.7113062103683623	0.0
11	1.0084386143246689E-4	0.0	0.0	4.304167271729836	0.0
12	1.0084386143246689E-4	0.0	0.0	4.891582764573955	0.0
13	1.0084386143246689E-4	0.0	0.0	5.17611371960566	0.0
14	1.0084386143246689E-4	0.0	0.0	5.298286057731094	0.0
15	1.0084386143246689E-4	0.0	0.0	5.433618519773464	0.0
16	1.0084386143246689E-4	0.0	0.0	5.737108120754473	0.0
17	1.0084386143246689E-4	0.0	0.0	6.088145602400891	0.0
18	1.0084386143246689E-4	0.0	0.0	6.62937460670894	0.0
19	1.0084386143246689E-4	0.0	0.0	6.831818658534618	0.0
20	1.0084386143246689E-4	0.0	0.0	7.070163125030253	0.0
21	1.0084386143246689E-4	0.0	0.0	7.331046194556045	0.0
22	1.0084386143246689E-4	0.0	0.0	7.601005211610759	0.0
23	1.0084386143246689E-4	0.0	0.0	7.918310421608016	0.0
24	1.0084386143246689E-4	0.0	0.0	8.147629362505446	0.0
25	1.0084386143246689E-4	0.0	0.0	8.33832510447424	0.0
26	1.0084386143246689E-4	0.0	0.0	8.519087726091938	0.0
27	1.0084386143246689E-4	0.0	0.0	8.731263210545848	0.0
28	1.0084386143246689E-4	0.0	0.0	8.920849670038885	0.0
29	1.0084386143246689E-4	0.0	0.0	9.128991400035497	0.0
30	1.0084386143246689E-4	0.0	0.0	9.410093663778499	0.0
31	1.5126579214870032E-4	0.0	0.0	9.638908385368765	0.0
32	1.5126579214870032E-4	0.0	0.0	9.832478177388387	0.0
33	1.5126579214870032E-4	0.0	0.0	10.028518644013102	0.0
34	1.5126579214870032E-4	0.0	0.0	10.236458686286849	0.0
35	1.5126579214870032E-4	0.0	0.0	10.489274246898043	0.0
36	1.5126579214870032E-4	0.0	0.0	10.700138761153331	0.0
37	1.5126579214870032E-4	0.0	0.0	10.918213611501042	0.0
38	1.5126579214870032E-4	0.0	0.0	11.135330445165142	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.857865E-4	44.0	1
CGACGGA	20	7.857865E-4	44.0	2
GACACGC	25	4.4440123E-5	44.0	9
CGCGAGT	60	0.0	44.0	4
CGTTATC	25	4.4440123E-5	44.0	17
CCGTAAG	25	4.4440123E-5	44.0	1
CGTCGGA	20	7.857865E-4	44.0	2
TTACGCG	25	4.4440123E-5	44.0	1
CCGGTTA	20	7.857865E-4	44.0	25
TGCTACG	50	2.7284841E-11	44.0	1
CTAGCGC	20	7.857865E-4	44.0	16
GAATCGT	35	1.4467878E-7	43.999996	9
TATCGTG	45	4.802132E-10	43.999996	1
CGACGGT	915	0.0	43.278687	28
CGGTCTA	940	0.0	42.3617	31
TCACGAC	945	0.0	42.137566	25
CGTTTTT	6590	0.0	42.03035	1
GAGCGAT	1265	0.0	41.913044	7
ACGGTCT	945	0.0	41.904762	30
CGCATGG	95	0.0	41.68421	2
>>END_MODULE