FastQCFastQC Report
Thu 26 May 2016
SRR1546775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546775_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1778813
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.2622535364875341No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34360.19316251905062534No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27480.1544850414293127No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAT207.8576454E-444.021
ACGTTAC254.4438268E-544.036
CGTTTTT54250.041.2829481
CGTAAGA1050.039.8095252
CGGTCTA2250.039.1111131
CGAATAT850.038.82353214
TCACGAC2250.038.13333525
GACCGAT9350.038.1176459
GTTAGCG1050.037.7142871
CTAAGCG1050.037.7142871
CGTTAGT357.292456E-637.71428743
TGTACGA700.037.7142872
GCGCGAC11150.037.6860969
CTACGGG2000.037.3999983
GAGCGAT11150.037.2914777
CGAGAAT1550.036.9032256
ATACGAG1850.036.8648643
GTTACGA601.9826984E-1036.6666682
GGTATCG301.3013816E-436.66666824
GTGATAG2700.036.6666641
Produced by FastQC (version 0.11.5)