FastQCFastQC Report
Thu 26 May 2016
SRR1546774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546774_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1787289
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86770.48548388089447203No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53880.3014621586100513No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29200.16337592857114883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA408.3164196E-944.02
GTAATCG254.443835E-544.01
CCGCGTA207.857656E-444.017
GCGTCTA254.443835E-544.020
CGTTTTT125200.043.1565481
CGGTCTA2250.042.04444531
CGACGGT2250.041.06666628
GAGCGAT12800.040.906257
CGCGTAA557.8216544E-1140.031
CGAGACC2000.039.66
GTCGTAC452.3521352E-839.111119
CGACACG800.038.513
CGAGGTA800.038.56
GTTGCGA800.038.52
TCGATTG750.038.133331
GTTTTTA57350.037.900612
AGCGATC1650.037.3333328
GTAGCGA950.037.052632
TCACGAC2500.036.9625
GACGGTC2500.036.9629
Produced by FastQC (version 0.11.5)