##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546774_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1787289
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.471343470473997	31.0	31.0	33.0	30.0	34.0
2	31.86737231639651	31.0	31.0	34.0	30.0	34.0
3	31.97629090762602	31.0	31.0	34.0	30.0	34.0
4	35.69269771145014	37.0	35.0	37.0	33.0	37.0
5	35.5787955948926	37.0	35.0	37.0	33.0	37.0
6	35.63243885012441	37.0	35.0	37.0	33.0	37.0
7	36.02074762391533	37.0	35.0	37.0	35.0	37.0
8	21.920858350272397	35.0	0.0	37.0	0.0	37.0
9	29.858075554652885	37.0	18.0	39.0	17.0	39.0
10	34.256720093952346	35.0	32.0	39.0	27.0	39.0
11	35.9046539199872	37.0	35.0	39.0	32.0	39.0
12	36.4540782156663	37.0	35.0	39.0	32.0	39.0
13	36.62948521475822	39.0	35.0	39.0	33.0	39.0
14	37.79236765850403	40.0	36.0	41.0	33.0	41.0
15	38.03306180477807	40.0	37.0	41.0	33.0	41.0
16	38.072747608249145	40.0	36.0	41.0	33.0	41.0
17	38.01879718389136	40.0	36.0	41.0	33.0	41.0
18	37.95543082288315	40.0	36.0	41.0	33.0	41.0
19	37.94546936729314	40.0	36.0	41.0	33.0	41.0
20	37.797664507530676	40.0	36.0	41.0	33.0	41.0
21	37.72663066801172	40.0	36.0	41.0	33.0	41.0
22	37.69322588568497	39.0	35.0	41.0	33.0	41.0
23	37.53160121278652	39.0	35.0	41.0	33.0	41.0
24	37.38873008226425	39.0	35.0	41.0	33.0	41.0
25	37.27515024151103	39.0	35.0	41.0	32.0	41.0
26	37.21222980726676	39.0	35.0	41.0	32.0	41.0
27	37.07911647193039	39.0	35.0	41.0	32.0	41.0
28	37.08822691797465	39.0	35.0	41.0	32.0	41.0
29	36.99433387661425	39.0	35.0	41.0	32.0	41.0
30	36.89730815777415	39.0	35.0	41.0	31.0	41.0
31	36.76900210318533	39.0	35.0	41.0	31.0	41.0
32	36.42763425500856	39.0	35.0	41.0	30.0	41.0
33	36.18824040208383	39.0	35.0	41.0	30.0	41.0
34	35.98564641756314	39.0	35.0	41.0	29.0	41.0
35	35.75384059321128	39.0	35.0	41.0	27.0	41.0
36	35.62698366072863	39.0	35.0	41.0	25.0	41.0
37	35.59502240544199	39.0	35.0	41.0	25.0	41.0
38	35.51700032843038	39.0	35.0	41.0	25.0	41.0
39	35.44288920258559	39.0	35.0	41.0	25.0	41.0
40	35.40298015597925	39.0	35.0	41.0	24.0	41.0
41	35.40383452256462	39.0	35.0	40.0	24.0	41.0
42	35.181018290830416	38.0	35.0	40.0	23.0	41.0
43	35.229687532346475	38.0	35.0	40.0	24.0	41.0
44	35.0387844383309	38.0	34.0	40.0	23.0	41.0
45	34.846567063300895	38.0	34.0	40.0	23.0	41.0
46	34.916847247423334	38.0	34.0	40.0	23.0	41.0
47	34.802363243996915	38.0	34.0	40.0	23.0	41.0
48	34.82185869213093	38.0	34.0	40.0	23.0	41.0
49	34.7662459736506	38.0	34.0	40.0	23.0	41.0
50	34.308725673352214	38.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	3.0
14	4.0
15	25.0
16	75.0
17	203.0
18	544.0
19	1274.0
20	2464.0
21	4371.0
22	7010.0
23	10477.0
24	15659.0
25	23920.0
26	31529.0
27	34304.0
28	34125.0
29	34899.0
30	40183.0
31	50108.0
32	63812.0
33	83704.0
34	123707.0
35	185315.0
36	168412.0
37	251637.0
38	302611.0
39	316907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.41623374843128	24.18360992542336	29.69413452441099	15.706021801734357
2	33.067959350726156	25.259485175592754	29.203223429450976	12.469332044230116
3	26.779608669890543	24.33680283378905	35.23520818401501	13.648380312305397
4	23.66315688173541	26.81659205646093	35.250035109039445	14.270215952764215
5	21.678195300256423	31.3109407600002	33.656280545563696	13.354583394179675
6	18.899125994732806	40.28369222884491	30.429661906943977	10.3875198694783
7	84.95262937331344	3.186222261760689	10.275842351181035	1.5853060137448391
8	52.45038715059513	40.27239019543006	5.700085436658537	1.5771372173162819
9	80.062317845631	5.2137622958570216	11.609202540831392	3.1147173176805767
10	38.7207664792879	31.873244897719395	18.213338749357266	11.192649873635434
11	29.88699645104961	24.635411508715155	30.38540493451255	15.09218710572269
12	27.387904250515728	22.95034546735307	32.59540007240015	17.066350209731052
13	20.853706367576816	31.22248276579781	32.83520460317274	15.088606263452636
14	16.891784149065987	33.80371053590102	32.44634751290922	16.858157802123774
15	15.59859653363278	25.414244702451587	43.13499383703475	15.85216492688088
16	17.507465216873154	23.36561126935823	40.60300264814476	18.523920865623857
17	17.947237408163986	23.72861915448481	32.913031971885914	25.411111465465293
18	20.71360591376101	24.494695597634184	35.5759477062747	19.21575078233011
19	23.746467415174603	26.432826476300143	30.91738381425724	18.903322294268023
20	25.185070797168223	24.978612860035508	30.602325645153076	19.23399069764319
21	21.572952107913157	27.785153939849682	31.899653609461033	18.742240342776125
22	21.048582517992333	24.747648533617113	31.22600765740739	22.97776129098316
23	18.14233736122138	29.34847134402998	30.71154133438968	21.797649960358957
24	18.322106833310116	25.460627799980866	37.232982466741525	18.984282899967493
25	17.15514390789626	27.756059596405507	35.005139068164134	20.083657427534103
26	17.743465102733804	30.734760858484556	32.35184684737611	19.16992719140553
27	18.53276106997805	29.630015067512865	32.59629528296767	19.240928579541418
28	16.993558400460138	27.703465975564107	37.26845518547924	18.034520438496514
29	17.26749283411916	25.538958724638267	36.56230189969277	20.6312465415498
30	18.583228565721605	28.113136711522312	33.60072153971741	19.70291318303867
31	22.67646698435452	26.62110044878025	30.1256819686128	20.576750598252435
32	22.72861299991216	28.007893519179046	30.02222919740456	19.241264283504233
33	22.19288542591601	27.754381076591418	29.838207475120136	20.214526022372432
34	18.806135997032378	28.79579071991155	30.445104289233583	21.952968993822488
35	18.925478755813973	27.513177779307096	31.535862415087877	22.02548104979105
36	22.66566850688389	27.231857858466093	29.08813292086506	21.014340713784957
37	19.669734441380214	29.96057156956709	29.947479115017213	20.42221487403548
38	19.60360076070518	30.042259533852665	27.720363075025915	22.633776630416232
39	20.1741296455134	29.982728031113044	28.47603269532795	21.367109628045604
40	21.45142727337325	27.478544320476434	28.429929351101023	22.640099055049294
41	18.75633990921446	27.10261183278138	29.214749265507706	24.926298992496456
42	20.86500840099167	27.791532315143215	28.083538812133906	23.259920471731206
43	20.532773379123352	28.006662604648717	28.939248213355533	22.521315802872394
44	19.82790695852769	30.185101570031485	29.58754851621646	20.399442955224366
45	18.460528767311835	31.293875808556983	28.269183103571947	21.976412320559238
46	21.19618036031106	30.1032457537645	28.50322471631616	20.197349169608273
47	20.722390167454734	27.690653274316578	29.27411291626592	22.312843641962772
48	20.816163474401733	26.233250470405178	30.136760199385776	22.813825855807316
49	19.96537773130143	25.64800656189346	31.321795188131297	23.064820518673812
50	19.316909576459096	28.616580754427517	30.031013451098282	22.035496218015105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	707.0
1	1137.0
2	1567.0
3	13512.5
4	25458.0
5	19154.5
6	12851.0
7	13127.0
8	13403.0
9	13914.5
10	14426.0
11	14478.5
12	14531.0
13	14121.0
14	13711.0
15	13267.5
16	12824.0
17	12303.5
18	11783.0
19	11504.5
20	11226.0
21	11632.0
22	12038.0
23	12994.5
24	13951.0
25	16079.0
26	18207.0
27	21975.5
28	25744.0
29	30503.5
30	35263.0
31	40446.0
32	45629.0
33	51111.5
34	56594.0
35	62766.0
36	68938.0
37	76891.5
38	84845.0
39	97489.5
40	110134.0
41	126280.0
42	142426.0
43	151097.0
44	159768.0
45	166173.5
46	172579.0
47	169323.0
48	166067.0
49	156045.0
50	146023.0
51	129642.0
52	113261.0
53	98541.0
54	83821.0
55	73406.0
56	62991.0
57	55301.5
58	47612.0
59	40600.5
60	33589.0
61	28451.0
62	23313.0
63	18864.0
64	14415.0
65	11122.5
66	7830.0
67	6050.5
68	4271.0
69	3367.5
70	2464.0
71	1914.0
72	1364.0
73	1068.0
74	772.0
75	618.0
76	464.0
77	359.0
78	254.0
79	180.0
80	106.0
81	71.5
82	37.0
83	28.0
84	19.0
85	11.0
86	3.0
87	2.5
88	2.0
89	2.5
90	3.0
91	2.0
92	1.0
93	2.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1787289.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.352739524701185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5602257751365	25.613480149561763
2	7.298470761708402	5.014449300112324
3	3.422765992524901	3.527441657856398
4	2.198328814714352	3.0207446864610894
5	1.6928607355073781	2.907720194923952
6	1.2930160084652371	2.6651185284045074
7	1.1265181082407083	2.7089288199576536
8	0.916057068616305	2.51752558943498
9	0.7816947506120556	2.416802054004198
>10	6.460685438226685	42.307000588692155
>50	0.22478254019512836	4.78080882218939
>100	0.023088284879925	1.3017256702554334
>500	0.0010038141148864597	0.24207602903578945
>1k	1.6730235248107664E-4	0.1678209887902434
>5k	3.346047049621533E-4	0.808356920320128
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8677	0.48548388089447203	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5388	0.3014621586100513	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2920	0.16337592857114883	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05309717678562337	0.0
2	0.0	0.0	0.0	0.1718804289625237	0.0
3	0.0	0.0	0.0	0.27723552262672685	0.0
4	0.0	0.0	0.0	0.39780919593865344	0.0
5	0.0	0.0	0.0	0.6604975468432918	0.0
6	0.0	0.0	0.0	1.0277576821655592	0.0
7	0.0	0.0	0.0	1.2911174409958321	0.0
8	0.0	0.0	0.0	1.9997325558429555	0.0
9	0.0	0.0	0.0	2.6713083334592223	0.0
10	0.0	0.0	0.0	3.677469060683527	0.0
11	0.0	0.0	0.0	4.2054194928744035	0.0
12	0.0	0.0	0.0	4.757988215671892	0.0
13	0.0	0.0	0.0	5.029348918949314	0.0
14	0.0	0.0	0.0	5.151768964056736	0.0
15	0.0	0.0	0.0	5.285491042579012	0.0
16	0.0	0.0	0.0	5.546668725650972	0.0
17	0.0	0.0	0.0	5.8427596208559445	0.0
18	0.0	0.0	0.0	6.314983195219128	0.0
19	0.0	0.0	0.0	6.5193709579144725	0.0
20	0.0	0.0	0.0	6.7548672878308995	0.0
21	0.0	0.0	0.0	6.978613979048716	0.0
22	0.0	0.0	0.0	7.222950513319335	0.0
23	0.0	0.0	0.0	7.4990110720762	0.0
24	0.0	0.0	0.0	7.698587077971162	0.0
25	0.0	0.0	0.0	7.871698421464016	0.0
26	0.0	0.0	0.0	8.037312376453947	0.0
27	0.0	0.0	0.0	8.222676914589638	0.0
28	5.5950660469571515E-5	0.0	0.0	8.40524391970185	0.0
29	5.5950660469571515E-5	0.0	0.0	8.602581899178029	0.0
30	5.5950660469571515E-5	0.0	0.0	8.853408710063118	0.0
31	5.5950660469571515E-5	0.0	0.0	9.079896983643943	0.0
32	5.5950660469571515E-5	0.0	0.0	9.291278578897984	0.0
33	5.5950660469571515E-5	0.0	0.0	9.501988766226392	0.0
34	5.5950660469571515E-5	0.0	0.0	9.706880084865961	0.0
35	5.5950660469571515E-5	0.0	0.0	9.932640999860682	0.0
36	5.5950660469571515E-5	0.0	0.0	10.150848575692011	0.0
37	5.5950660469571515E-5	0.0	0.0	10.379854628993968	0.0
38	5.5950660469571515E-5	0.0	0.0	10.602706109644272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	40	8.3164196E-9	44.0	2
GTAATCG	25	4.443835E-5	44.0	1
CCGCGTA	20	7.857656E-4	44.0	17
GCGTCTA	25	4.443835E-5	44.0	20
CGTTTTT	12520	0.0	43.156548	1
CGGTCTA	225	0.0	42.044445	31
CGACGGT	225	0.0	41.066666	28
GAGCGAT	1280	0.0	40.90625	7
CGCGTAA	55	7.8216544E-11	40.0	31
CGAGACC	200	0.0	39.6	6
GTCGTAC	45	2.3521352E-8	39.11111	9
CGACACG	80	0.0	38.5	13
CGAGGTA	80	0.0	38.5	6
GTTGCGA	80	0.0	38.5	2
TCGATTG	75	0.0	38.13333	1
GTTTTTA	5735	0.0	37.90061	2
AGCGATC	165	0.0	37.333332	8
GTAGCGA	95	0.0	37.05263	2
TCACGAC	250	0.0	36.96	25
GACGGTC	250	0.0	36.96	29
>>END_MODULE