FastQCFastQC Report
Thu 26 May 2016
SRR1546773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546773_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1869400
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48240.2580507114582219No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA37900.20273884668877717No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29390.1572162191077351No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG207.857749E-444.023
GTCGAAC207.857749E-444.039
ACGTTAA207.857749E-444.040
CTACGCG302.5285717E-644.01
ACTACGC207.857749E-444.026
CGAATAC207.857749E-444.035
CGTCGTA254.4439133E-544.030
TCGACGA207.857749E-444.02
CTAGCGA900.044.02
CGTTTTT68900.042.371551
TTTAGCG1250.042.241
GAGCGAT12900.041.6124047
TATAGCG900.041.5555571
AGCGATC1600.041.258
GTTAGCG750.041.0666661
AGCGATA2650.040.6792458
ATCGCCG603.6379788E-1240.33333221
CGCGTAA452.3523171E-839.1111131
GCGTAAC404.1263957E-738.532
AGTACGA1100.038.02
Produced by FastQC (version 0.11.5)