FastQCFastQC Report
Thu 26 May 2016
SRR1546772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546772_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4179538
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135280.3236721379252922No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86290.2064582257656229No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA44010.10529871961924978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT201550.042.6137471
CGACGGT4000.040.1528
GAGCGAT30600.040.045757
CGGTCTA4200.039.28571331
CGCCGTT452.3534085E-839.1111126
TCACGAC4300.038.37209325
TACGGGT2300.038.260874
GTAAGCG2700.037.481481
TAGGGCG11650.037.2017175
GCGATAA4500.037.1555569
TCGATTG1600.037.1251
GCGCGAC8900.036.8314639
ACGAGAT10650.036.3568085
CGGCTAC7350.036.2176925
GGGCGAT57150.036.1469847
GACCGAT20150.036.0297789
TACGGGA13250.035.6981124
CACGACG4500.035.6888926
AGCGATA8250.035.4666678
AGGGCGA28400.035.401416
Produced by FastQC (version 0.11.5)