FastQCFastQC Report
Thu 26 May 2016
SRR1546771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546771_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3375736
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162390.4810506508802821No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103320.3060665881455185No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45100.13360049482542471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT250850.043.1756021
GAGCGAT22250.042.2202267
CGACGGT2350.042.12766328
GAGACCG7450.040.1610767
AGCGATA5650.040.1061978
AACGCGA1000.039.62
TAACGCG800.038.5000041
GTAAGCG1550.038.3225821
GACCGAT15250.037.9409839
GTTTTTA116950.037.905092
CTAGCGA2050.037.5609742
ACGAGAC4350.037.4252855
CGAGATT6350.036.724416
TTTCGCG1200.036.6666641
ACGAGAT7250.036.4137955
TACGGGA9250.036.1513524
TAGCGCG1400.036.1428571
CACGACG2750.036.026
CGGTCTA2750.036.031
TGCGCGA1350.035.8518522
Produced by FastQC (version 0.11.5)