##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546771_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3375736
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.414427550021685	31.0	31.0	33.0	30.0	34.0
2	31.8057685790595	31.0	31.0	34.0	30.0	34.0
3	31.912574620764182	31.0	31.0	34.0	30.0	34.0
4	35.63654385295533	37.0	35.0	37.0	33.0	37.0
5	35.524691504311946	37.0	35.0	37.0	33.0	37.0
6	35.57652316413369	37.0	35.0	37.0	33.0	37.0
7	35.999822853445885	37.0	35.0	37.0	35.0	37.0
8	21.888020567959106	35.0	0.0	37.0	0.0	37.0
9	29.82688278941244	37.0	18.0	39.0	17.0	39.0
10	34.18511311311074	35.0	32.0	39.0	27.0	39.0
11	35.85228850834307	37.0	35.0	39.0	32.0	39.0
12	36.43517650669365	37.0	35.0	39.0	32.0	39.0
13	36.62786100571846	39.0	35.0	39.0	33.0	39.0
14	37.77904137053371	40.0	36.0	41.0	33.0	41.0
15	38.008645818274886	40.0	37.0	41.0	33.0	41.0
16	38.03228392267642	40.0	36.0	41.0	33.0	41.0
17	37.99789912481308	40.0	36.0	41.0	33.0	41.0
18	37.92264620219117	40.0	36.0	41.0	33.0	41.0
19	37.90946863143326	40.0	36.0	41.0	33.0	41.0
20	37.775249901058615	40.0	36.0	41.0	33.0	41.0
21	37.68682562854441	39.0	36.0	41.0	33.0	41.0
22	37.64692144172412	39.0	35.0	41.0	33.0	41.0
23	37.48267192695163	39.0	35.0	41.0	32.0	41.0
24	37.339380508428384	39.0	35.0	41.0	32.0	41.0
25	37.188188590576985	39.0	35.0	41.0	32.0	41.0
26	37.134507556278095	39.0	35.0	41.0	32.0	41.0
27	37.01779848898136	39.0	35.0	41.0	32.0	41.0
28	36.99183792808442	39.0	35.0	41.0	32.0	41.0
29	36.91611370083442	39.0	35.0	41.0	31.0	41.0
30	36.7752724146675	39.0	35.0	41.0	31.0	41.0
31	36.679364144589506	39.0	35.0	41.0	31.0	41.0
32	36.341436652629234	39.0	35.0	41.0	30.0	41.0
33	36.10553254164425	39.0	35.0	41.0	29.0	41.0
34	35.92400827552866	39.0	35.0	41.0	27.0	41.0
35	35.70761961243415	39.0	35.0	41.0	26.0	41.0
36	35.57991412835601	39.0	35.0	41.0	25.0	41.0
37	35.56105394497674	39.0	35.0	41.0	25.0	41.0
38	35.473396912554776	39.0	35.0	41.0	25.0	41.0
39	35.379660020807314	39.0	35.0	41.0	24.0	41.0
40	35.32426647107475	39.0	35.0	40.0	24.0	41.0
41	35.323300459514606	39.0	35.0	40.0	24.0	41.0
42	35.068720717496866	38.0	35.0	40.0	23.0	41.0
43	35.11542786521221	38.0	35.0	40.0	23.0	41.0
44	34.90040305284536	38.0	34.0	40.0	23.0	41.0
45	34.72793784822036	38.0	34.0	40.0	22.0	41.0
46	34.825790879381564	38.0	34.0	40.0	23.0	41.0
47	34.71135479788704	38.0	34.0	40.0	23.0	41.0
48	34.7188764168762	38.0	34.0	40.0	23.0	41.0
49	34.64055038664161	38.0	34.0	40.0	22.0	41.0
50	34.13435855173509	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	4.0
14	11.0
15	43.0
16	155.0
17	448.0
18	1148.0
19	2722.0
20	5049.0
21	8706.0
22	13645.0
23	20527.0
24	31089.0
25	46243.0
26	61622.0
27	67634.0
28	66619.0
29	68993.0
30	79178.0
31	97276.0
32	123537.0
33	160619.0
34	231973.0
35	338242.0
36	319180.0
37	474373.0
38	564155.0
39	592539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.821130562342553	24.170462382129408	29.37732689997085	15.63108015555719
2	33.56284377688303	24.567323985050965	29.038497086265043	12.831335151800971
3	26.368116464083684	24.719675946223283	35.54303417091858	13.369173418774455
4	23.601519787092357	26.919077795183032	35.577130439110164	13.902271978614442
5	21.171294200731335	31.221102598070466	34.318767818336504	13.288835382861693
6	18.98522277808454	39.919679737988986	30.663772285510476	10.43132519841599
7	84.23223853998061	3.3998215500264237	10.521498126630755	1.846441783362206
8	51.79306083177121	40.25291077264336	6.100062327148806	1.8539660684366313
9	79.00478591927805	5.366888879936109	12.50986451547159	3.1184606853142545
10	36.51968044894506	33.24774212201428	18.791161394137458	11.441416034903204
11	28.679760502598544	25.07379131543462	31.610973132970116	14.635475048996721
12	25.835699237144137	23.6328611005126	33.36795294418758	17.163486718155685
13	20.6129270772359	29.87538125019255	34.42126398509836	15.09042768747319
14	17.24071432126209	32.9843921444094	33.014489284707096	16.760404249621416
15	15.69317031900599	25.879037934246043	42.731451748596456	15.696339998151515
16	17.4593333127946	24.29926392348217	40.74059701351054	17.500805750212695
17	18.44060080527624	23.966003265658216	33.53280588292449	24.060590046141048
18	20.519851078401867	24.81041171466015	36.14112596482663	18.52861124211135
19	23.01906902672484	27.083930733919953	31.72303758350771	18.173962655847493
20	23.933477025454597	25.795826450883602	31.202380754893156	19.06831576876865
21	21.367221844362234	28.32028333969244	32.37649508136892	17.9359997345764
22	20.75911741913467	25.027934648918045	31.719512426327178	22.493435505620106
23	17.77322041771039	29.36058980915569	31.238461775446897	21.627727997687025
24	18.176836103297177	26.416876201219523	36.378466799536454	19.02782089594684
25	17.46362867238433	27.79396848568727	34.92068100112094	19.821721840807456
26	17.690719890417974	30.61279081065581	32.55275294039581	19.143736358530404
27	18.78286690665384	29.552310962705615	32.78680560328177	18.878016527358774
28	17.147756815106394	27.91311287375553	37.350728848464456	17.588401462673623
29	17.891446487521538	25.863189538518416	35.42445262307242	20.820911350887627
30	19.09773157616591	27.85374804190849	32.90141764640363	20.147102735521973
31	22.143111902115567	26.566591700298837	30.593713489443488	20.696582908142105
32	22.317858979493657	26.80248099969903	31.093100882296483	19.78655913851083
33	21.280899928193435	28.088393168186137	30.131029203705502	20.499677699914923
34	18.52102771069776	27.09483206032699	32.42824675863278	21.955893470342467
35	18.553079980188024	27.329506809774223	32.683331871923635	21.43408133811412
36	22.06807641355841	26.10974317896897	30.89906912151898	20.92311128595364
37	19.358030367303606	28.867127050219565	32.55778887922515	19.217053703251676
38	19.386675972291673	29.206756689504154	29.42433887010122	21.982228468102956
39	20.549948218699566	28.15614135702555	30.406435811331217	20.887474612943667
40	21.30133991520664	26.42330442901933	29.668285671628354	22.60706998414568
41	19.20523405858752	26.32131185614041	29.454850734773096	25.018603350498974
42	21.246625920984343	26.77193951185756	28.725498676436782	23.255935890721315
43	21.074219074003416	27.470039126282387	29.631819549870013	21.823922249844184
44	20.499470337727832	29.480652515481072	29.920023366756165	20.09985378003493
45	18.45493841935507	30.587699986017864	29.332418174880974	21.624943419746092
46	20.816556744958728	29.52538350155344	28.8394293866582	20.818630366829634
47	20.211799737894196	26.988129403484155	30.03587958300057	22.76419127562108
48	21.07436718985134	26.042854062047503	30.41055343190344	22.47222531619771
49	21.321898394898177	24.562939755952478	31.27445392649188	22.840707922657458
50	19.42207565994497	28.38622451518721	30.476198375702367	21.715501449165455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1275.0
1	1980.0
2	2685.0
3	25264.0
4	47843.0
5	36415.5
6	24988.0
7	25437.5
8	25887.0
9	26956.5
10	28026.0
11	28225.0
12	28424.0
13	27955.5
14	27487.0
15	26316.5
16	25146.0
17	24372.0
18	23598.0
19	23105.5
20	22613.0
21	22832.0
22	23051.0
23	24963.5
24	26876.0
25	31549.5
26	36223.0
27	43430.5
28	50638.0
29	59770.5
30	68903.0
31	79829.0
32	90755.0
33	100675.0
34	110595.0
35	120709.5
36	130824.0
37	145932.5
38	161041.0
39	185800.5
40	210560.0
41	239195.5
42	267831.0
43	287964.5
44	308098.0
45	322250.0
46	336402.0
47	332429.5
48	328457.0
49	305020.5
50	281584.0
51	242328.0
52	203072.0
53	175756.5
54	148441.0
55	128797.5
56	109154.0
57	93578.5
58	78003.0
59	67647.0
60	57291.0
61	48387.0
62	39483.0
63	31424.0
64	23365.0
65	17607.5
66	11850.0
67	9071.0
68	6292.0
69	5025.0
70	3758.0
71	3043.0
72	2328.0
73	1875.0
74	1422.0
75	1062.0
76	702.0
77	575.5
78	449.0
79	330.5
80	212.0
81	131.0
82	50.0
83	40.5
84	31.0
85	24.5
86	18.0
87	10.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3375736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.78946870740263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51331669163359	24.64107155375676
2	7.390899588507535	4.699055423768305
3	2.8840173378200946	2.7504413673671566
4	1.6865375489690366	2.1445653054724287
5	1.1647442507730543	1.8513300456038564
6	0.9257375858935402	1.765722361081749
7	0.709145242248421	1.5780345341221746
8	0.631476168206443	1.6059433510935393
9	0.5518125813960298	1.5787645909776142
>10	5.87281886836671	39.895082776344175
>50	0.5991472179517452	12.707593938525127
>100	0.06802686429756451	3.2251743689674144
>500	0.0017148224745953213	0.3981394562486996
>1k	4.03487641080591E-4	0.30703811965017647
>5k	0.0	0.0
>10k+	2.017438205402955E-4	0.8520428070208709
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16239	0.4810506508802821	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10332	0.3060665881455185	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4510	0.13360049482542471	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07035502776283453	0.0
2	0.0	0.0	0.0	0.21476797948654752	0.0
3	0.0	0.0	0.0	0.33290517978894085	0.0
4	0.0	0.0	0.0	0.4718674683091332	0.0
5	0.0	0.0	0.0	0.7736979432040895	0.0
6	0.0	0.0	0.0	1.2441731225427581	0.0
7	0.0	0.0	0.0	1.5912381773930189	0.0
8	0.0	0.0	0.0	2.5460225562662484	0.0
9	0.0	0.0	0.0	3.468547303462119	0.0
10	0.0	0.0	0.0	4.732271717930549	0.0
11	0.0	0.0	0.0	5.3806636537928325	0.0
12	0.0	0.0	0.0	6.043689435429785	0.0
13	0.0	0.0	0.0	6.389065969613737	0.0
14	0.0	0.0	0.0	6.540203380833098	0.0
15	0.0	0.0	0.0	6.694214239502141	0.0
16	0.0	0.0	0.0	7.000103088630153	0.0
17	0.0	0.0	0.0	7.334785658594155	0.0
18	0.0	0.0	0.0	7.869957840305047	0.0
19	0.0	0.0	0.0	8.094116364549835	0.0
20	0.0	0.0	0.0	8.370204305075989	0.0
21	0.0	0.0	0.0	8.636605469148062	0.0
22	0.0	0.0	0.0	8.912960018200476	0.0
23	0.0	0.0	0.0	9.23200155462394	0.0
24	0.0	0.0	0.0	9.471267895356746	0.0
25	2.9623169584351384E-5	0.0	0.0	9.678156111733855	0.0
26	2.9623169584351384E-5	0.0	0.0	9.880097258790379	0.0
27	2.9623169584351384E-5	0.0	0.0	10.101411958755069	0.0
28	2.9623169584351384E-5	0.0	0.0	10.324978019608169	0.0
29	2.9623169584351384E-5	0.0	0.0	10.557934625219508	0.0
30	2.9623169584351384E-5	0.0	0.0	10.859113390383609	0.0
31	2.9623169584351384E-5	0.0	0.0	11.119678790047564	0.0
32	2.9623169584351384E-5	0.0	0.0	11.36282576599592	0.0
33	5.924633916870277E-5	0.0	0.0	11.60458045297381	0.0
34	5.924633916870277E-5	0.0	0.0	11.853503946991115	0.0
35	5.924633916870277E-5	0.0	0.0	12.11475067955551	0.0
36	5.924633916870277E-5	0.0	0.0	12.360474871257704	0.0
37	5.924633916870277E-5	0.0	0.0	12.620003459986208	0.0
38	5.924633916870277E-5	0.0	0.0	12.88225738031647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	25085	0.0	43.175602	1
GAGCGAT	2225	0.0	42.220226	7
CGACGGT	235	0.0	42.127663	28
GAGACCG	745	0.0	40.161076	7
AGCGATA	565	0.0	40.106197	8
AACGCGA	100	0.0	39.6	2
TAACGCG	80	0.0	38.500004	1
GTAAGCG	155	0.0	38.322582	1
GACCGAT	1525	0.0	37.940983	9
GTTTTTA	11695	0.0	37.90509	2
CTAGCGA	205	0.0	37.560974	2
ACGAGAC	435	0.0	37.425285	5
CGAGATT	635	0.0	36.72441	6
TTTCGCG	120	0.0	36.666664	1
ACGAGAT	725	0.0	36.413795	5
TACGGGA	925	0.0	36.151352	4
TAGCGCG	140	0.0	36.142857	1
CACGACG	275	0.0	36.0	26
CGGTCTA	275	0.0	36.0	31
TGCGCGA	135	0.0	35.851852	2
>>END_MODULE