FastQCFastQC Report
Thu 26 May 2016
SRR1546770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546770_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2306511
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70610.30613337634201615No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA47980.20801981867851488No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41640.18053241454300456No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTC207.8581326E-444.029
CGTTTTT106800.042.661051
CGGTCTA2700.040.7407431
TAACGCG557.8216544E-1140.0000041
CCGACTA557.8216544E-1140.00000418
TCGACGA501.3496901E-939.6000022
CACGACG3000.038.86666526
GTTGCGA850.038.8235322
ACGGTCT2900.038.68965530
AGCGATA4000.038.58
TCTAGCG700.037.7142871
GAGCGAT18200.037.7142877
TATTACG700.037.7142871
ACGAGTA1400.037.7142875
CGACGGT3050.037.50819828
TAGACGG2000.037.42
TATCGCG651.0913936E-1137.2307661
CGTTAGA1600.037.1252
TACGATG601.9826984E-1036.6666681
ACGTAGA1200.036.6666682
Produced by FastQC (version 0.11.5)