##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546770_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2306511
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.473096378035915	31.0	31.0	33.0	30.0	34.0
2	31.876922329873995	31.0	31.0	34.0	30.0	34.0
3	32.00853496905066	33.0	31.0	34.0	30.0	34.0
4	35.70794416328385	37.0	35.0	37.0	35.0	37.0
5	35.59634140049625	37.0	35.0	37.0	33.0	37.0
6	35.64973199780968	37.0	35.0	37.0	33.0	37.0
7	36.01414170580587	37.0	35.0	37.0	35.0	37.0
8	22.039142670466344	35.0	0.0	37.0	0.0	37.0
9	29.929146663510384	37.0	18.0	39.0	17.0	39.0
10	34.23931643941867	35.0	32.0	39.0	27.0	39.0
11	35.93134999139393	37.0	35.0	39.0	32.0	39.0
12	36.336110254839454	37.0	35.0	39.0	32.0	39.0
13	36.46438018288229	39.0	35.0	39.0	32.0	39.0
14	37.577627420810046	39.0	36.0	41.0	33.0	41.0
15	37.82744673665116	40.0	36.0	41.0	33.0	41.0
16	37.87531210560019	40.0	36.0	41.0	33.0	41.0
17	37.815740744353704	40.0	36.0	41.0	33.0	41.0
18	37.78015019221673	39.0	36.0	41.0	33.0	41.0
19	37.77387491323475	39.0	36.0	41.0	33.0	41.0
20	37.658325496821824	39.0	35.0	41.0	33.0	41.0
21	37.57454397572784	39.0	35.0	41.0	33.0	41.0
22	37.53289058669133	39.0	35.0	41.0	33.0	41.0
23	37.40554673270581	39.0	35.0	41.0	32.0	41.0
24	37.308643227801646	39.0	35.0	41.0	32.0	41.0
25	37.166766167601196	39.0	35.0	41.0	32.0	41.0
26	37.105673460911305	39.0	35.0	41.0	32.0	41.0
27	36.99704792216469	39.0	35.0	41.0	32.0	41.0
28	37.00249771191206	39.0	35.0	41.0	32.0	41.0
29	36.908118365791445	39.0	35.0	41.0	32.0	41.0
30	36.8194558794647	39.0	35.0	41.0	31.0	41.0
31	36.70534760077017	39.0	35.0	40.0	31.0	41.0
32	36.463282854493215	39.0	35.0	41.0	30.0	41.0
33	36.30609955903093	39.0	35.0	41.0	30.0	41.0
34	36.18434336536873	39.0	35.0	41.0	30.0	41.0
35	36.02176664234422	39.0	35.0	41.0	29.0	41.0
36	35.9000893557412	39.0	35.0	41.0	29.0	41.0
37	35.86973961971133	39.0	35.0	41.0	29.0	41.0
38	35.80895647148442	38.0	35.0	40.0	29.0	41.0
39	35.71304407392811	38.0	35.0	40.0	28.0	41.0
40	35.664936781138266	38.0	35.0	40.0	28.0	41.0
41	35.669302249154676	38.0	35.0	40.0	28.0	41.0
42	35.440692457135476	38.0	35.0	40.0	27.0	41.0
43	35.47776360052044	38.0	35.0	40.0	27.0	41.0
44	35.27648773407107	38.0	34.0	40.0	26.0	41.0
45	35.10140510927544	38.0	34.0	40.0	26.0	41.0
46	35.18210101751087	38.0	34.0	40.0	26.0	41.0
47	35.058323589178634	38.0	34.0	40.0	26.0	41.0
48	35.08037941288812	38.0	34.0	40.0	26.0	41.0
49	35.00490914632534	38.0	34.0	40.0	26.0	41.0
50	34.53781880944856	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	4.0
13	8.0
14	13.0
15	32.0
16	90.0
17	242.0
18	658.0
19	1439.0
20	2776.0
21	4974.0
22	7898.0
23	11700.0
24	17275.0
25	24607.0
26	32708.0
27	37251.0
28	39084.0
29	43979.0
30	52570.0
31	66831.0
32	87277.0
33	116219.0
34	178492.0
35	276427.0
36	221429.0
37	323941.0
38	385280.0
39	373303.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.57552684552556	25.321622138372636	29.751733245581747	13.351117770520062
2	32.172662519276955	25.554528029565	29.121257171546116	13.151552279611934
3	27.906478659759266	25.228711243952446	32.93372544071977	13.931084655568519
4	25.253597316466298	27.20559320983078	32.96866999550403	14.57213947819889
5	22.518210405239774	31.765120565217337	31.85811817069158	13.858550858851313
6	19.398216613751245	40.28374458218495	29.10582260392428	11.212216200139519
7	86.08768828763445	4.123847664286015	7.969612978216882	1.8188510698626628
8	53.57789319019073	40.209129720170424	4.5172123610076	1.695764728631253
9	81.71146810052066	5.300516667815588	9.701102661118894	3.286912570544862
10	44.26972167052314	24.154881550532384	18.067071867422264	13.508324911522207
11	38.02344753612707	23.957743969137802	22.905678750285606	15.113129744449516
12	33.01649114181549	21.865449590311947	28.540596598065214	16.577462669807343
13	23.176043816829836	32.474720476078375	28.876038310677902	15.473197396413891
14	18.185952722531997	36.04400759415412	29.84143583100189	15.928603852311998
15	16.994326062177894	24.388654552265304	42.85069527090918	15.76632411464762
16	18.76067358881011	21.811341892581478	40.57045468241859	18.857529836189812
17	19.347447291601906	22.076417584828338	30.535167618970817	28.040967504598935
18	23.3343782015347	23.996503810300492	32.50420223445715	20.164915753707657
19	27.17537440749253	26.426494389144466	27.04075549607177	19.35737570729123
20	28.496764160240296	24.8036970125007	26.89781232346171	19.801726503797294
21	23.75401634763502	28.30222791046737	28.53378978032188	19.409965961575733
22	22.781812009567698	25.5272140475376	27.507651166632197	24.183322776262504
23	20.26974941806044	29.69428717227015	27.070800876301917	22.965162533367497
24	21.4743393809958	25.132245196315996	34.16506576383117	19.228349658857034
25	19.69143004303903	25.99918231476026	32.65989193201333	21.649495710187377
26	18.61430532956487	30.77652783793357	29.72051726612186	20.888649566379698
27	19.708382054106828	30.133608727641015	29.420627085671825	20.737382132580333
28	18.100759111922727	28.091823537802334	35.106791166398075	18.70062618387686
29	19.054103795733035	26.426971299941776	33.90155087055731	20.617374033767884
30	20.61915161037602	28.926937699408327	30.328535177157185	20.12537551305847
31	25.877960261191035	26.8172577542444	26.737613651094662	20.567168333469905
32	27.388033267563	27.41647449329312	26.817084332136286	18.378407907007595
33	25.592637537822277	28.351219656008574	26.06178769578814	19.994355110381004
34	21.629161968011427	28.585252790903663	28.344803038008493	21.440782203076424
35	21.23289245097899	28.33201315753534	29.008012534949973	21.427081856535693
36	27.451809247820623	27.19223970750627	26.343858754629828	19.012092290043274
37	22.13958658770758	31.00904352938269	27.545370475146225	19.3059994077635
38	23.27606501768255	31.22356667711535	24.421344619644128	21.07902368555797
39	22.321679801223578	29.63948578610724	26.7204015068647	21.31843290580448
40	24.213801711762915	26.170393290992326	26.126083942370098	23.489721054874657
41	19.29472697073632	25.357260381589334	27.89832782067807	27.449684826996272
42	22.42933157483316	27.004900475219934	25.655676474120437	24.910091475826476
43	22.039912231071085	26.769393252405905	27.170735366100573	24.019959150422434
44	21.279889842276926	29.25171395237222	27.61577985103908	21.85261635431177
45	20.114016364977232	31.55961536710642	25.52543647092947	22.80093179698688
46	22.73243006428324	30.068748859207695	26.561416789254423	20.637404287254647
47	21.695842768579904	27.269585967723543	27.82236893732568	23.21220232637087
48	23.051743520841654	25.270766105169233	29.032898607463824	22.644591766525284
49	21.96083174977271	24.14699084461336	30.251752538791276	23.64042486682266
50	20.546704524712865	28.349138590711252	28.118573897978376	22.985582986597507
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1150.0
1	1825.5
2	2501.0
3	12303.5
4	22106.0
5	16483.0
6	10860.0
7	11158.5
8	11457.0
9	12059.0
10	12661.0
11	13054.5
12	13448.0
13	13067.0
14	12686.0
15	12281.0
16	11876.0
17	11767.5
18	11659.0
19	11363.5
20	11068.0
21	11403.5
22	11739.0
23	12917.0
24	14095.0
25	16065.5
26	18036.0
27	21774.5
28	25513.0
29	29647.0
30	33781.0
31	38996.0
32	44211.0
33	50259.0
34	56307.0
35	62993.5
36	69680.0
37	78496.5
38	87313.0
39	106959.5
40	126606.0
41	149665.0
42	172724.0
43	187756.0
44	202788.0
45	210085.0
46	217382.0
47	214881.5
48	212381.0
49	203876.0
50	195371.0
51	176765.0
52	158159.0
53	141539.5
54	124920.0
55	113188.5
56	101457.0
57	94755.5
58	88054.0
59	80996.5
60	73939.0
61	64852.0
62	55765.0
63	46095.0
64	36425.0
65	29286.5
66	22148.0
67	17581.0
68	13014.0
69	10873.5
70	8733.0
71	7578.0
72	6423.0
73	5256.0
74	4089.0
75	3065.5
76	2042.0
77	1556.5
78	1071.0
79	788.0
80	505.0
81	369.0
82	233.0
83	152.0
84	71.0
85	59.0
86	47.0
87	26.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	2.5
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2306511.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.204566754990775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86500644800553	22.44809211933574
2	6.948691045550326	4.058670229991622
3	2.7986177035120865	2.4519725263175314
4	1.7006529203130976	1.9866732695341547
5	1.2055029725024995	1.7603096016894526
6	0.9259884397682417	1.6225854722136823
7	0.7661533547719857	1.566262375579901
8	0.6242265501872456	1.458421276414481
9	0.5715936607247708	1.5023830699229503
>10	6.816816774775646	43.55340394999665
>50	0.7054501283846504	13.593862284855282
>100	0.0694114378702093	2.9625240897457714
>500	0.0014164227253153816	0.29838683347739503
>1k	3.147606056256403E-4	0.4119135553950115
>5k	1.5738030281282016E-4	0.3245393455304816
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7061	0.30613337634201615	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4798	0.20801981867851488	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4164	0.18053241454300456	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044526126257364476	0.0
2	0.0	0.0	0.0	0.1674390453806637	0.0
3	0.0	0.0	0.0	0.2803801932876106	0.0
4	0.0	0.0	0.0	0.40736853195150596	0.0
5	0.0	0.0	0.0	0.6648136514415063	0.0
6	0.0	0.0	0.0	1.0347663635681772	0.0
7	0.0	0.0	0.0	1.279074758368809	0.0
8	0.0	0.0	0.0	1.924811977918163	0.0
9	0.0	0.0	0.0	2.5211238966560314	0.0
10	0.0	0.0	0.0	3.434841628763097	0.0
11	0.0	0.0	0.0	4.01450502512236	0.0
12	0.0	0.0	0.0	4.555061736102711	0.0
13	0.0	0.0	0.0	4.817839585417109	0.0
14	0.0	0.0	0.0	4.930173755945669	0.0
15	0.0	0.0	0.0	5.059113093325807	0.0
16	0.0	0.0	0.0	5.330085137248424	0.0
17	0.0	0.0	0.0	5.633140271171479	0.0
18	0.0	0.0	0.0	6.114950243029407	0.0
19	0.0	0.0	0.0	6.311220713883437	0.0
20	0.0	0.0	0.0	6.548939068575871	0.0
21	0.0	0.0	0.0	6.779503761308748	0.0
22	0.0	0.0	0.0	7.020473780528253	0.0
23	0.0	0.0	0.0	7.298642841937454	0.0
24	0.0	0.0	0.0	7.500159331561827	0.0
25	0.0	0.0	0.0	7.672107351753363	0.0
26	0.0	0.0	0.0	7.836077954971817	0.0
27	0.0	0.0	0.0	8.028056228650113	0.0
28	0.0	0.0	0.0	8.214615061449956	0.0
29	0.0	0.0	0.0	8.4024312045336	0.0
30	0.0	0.0	0.0	8.634643407293527	0.0
31	0.0	0.0	0.0	8.848212733431577	0.0
32	0.0	0.0	0.0	9.04205529477206	0.0
33	0.0	0.0	0.0	9.245219294423482	0.0
34	0.0	0.0	0.0	9.44955389330465	0.0
35	0.0	0.0	0.0	9.66949648191576	0.0
36	0.0	0.0	0.0	9.884409829391666	0.0
37	0.0	0.0	0.0	10.109815214408256	0.0
38	0.0	0.0	0.0	10.34024984056005	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCGTC	20	7.8581326E-4	44.0	29
CGTTTTT	10680	0.0	42.66105	1
CGGTCTA	270	0.0	40.74074	31
TAACGCG	55	7.8216544E-11	40.000004	1
CCGACTA	55	7.8216544E-11	40.000004	18
TCGACGA	50	1.3496901E-9	39.600002	2
CACGACG	300	0.0	38.866665	26
GTTGCGA	85	0.0	38.823532	2
ACGGTCT	290	0.0	38.689655	30
AGCGATA	400	0.0	38.5	8
TCTAGCG	70	0.0	37.714287	1
GAGCGAT	1820	0.0	37.714287	7
TATTACG	70	0.0	37.714287	1
ACGAGTA	140	0.0	37.714287	5
CGACGGT	305	0.0	37.508198	28
TAGACGG	200	0.0	37.4	2
TATCGCG	65	1.0913936E-11	37.230766	1
CGTTAGA	160	0.0	37.125	2
TACGATG	60	1.9826984E-10	36.666668	1
ACGTAGA	120	0.0	36.666668	2
>>END_MODULE