FastQCFastQC Report
Thu 26 May 2016
SRR1546769_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546769_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2809001
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74850.2664648392791601No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA49730.17703802882234643No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.1651832804616303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC207.858426E-444.032
CGTTTTT108550.042.561031
CGACGGT6200.041.87096828
GAGCGAT17550.041.6182377
CGGTCTA6350.040.8818931
AGCGATA4200.040.857148
CACGACG6500.039.9384626
CGTAAGA2250.039.111112
CGTTAGA1300.038.923082
CCAATCG850.038.82353224
CTCACGA6850.038.54014624
AACGCGA800.038.52
TCACGAC6700.038.4179125
ACGAGAT4550.038.1978035
GACGGTC6850.037.8978129
TGCGAAG2050.037.5609781
ACGACGG6950.037.35251627
CTATGCG950.037.0526351
ACGTTAG1250.036.961
TGCGCGA1250.036.962
Produced by FastQC (version 0.11.5)