##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546769_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2809001
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.390919049156622	31.0	31.0	33.0	30.0	34.0
2	31.780366044725508	31.0	31.0	34.0	30.0	34.0
3	31.923197606551227	31.0	31.0	34.0	30.0	34.0
4	35.63450173211046	37.0	35.0	37.0	33.0	37.0
5	35.509592200216375	37.0	35.0	37.0	33.0	37.0
6	35.55854376698335	37.0	35.0	37.0	33.0	37.0
7	35.97157067583814	37.0	35.0	37.0	35.0	37.0
8	21.93624388172165	35.0	0.0	37.0	0.0	37.0
9	29.846270257646758	37.0	18.0	39.0	17.0	39.0
10	34.174640379266506	35.0	32.0	39.0	27.0	39.0
11	35.81945752244303	37.0	35.0	39.0	32.0	39.0
12	36.27027117469876	37.0	35.0	39.0	32.0	39.0
13	36.39040285140518	38.0	35.0	39.0	32.0	39.0
14	37.49957262386165	39.0	36.0	41.0	33.0	41.0
15	37.77068929487743	40.0	36.0	41.0	33.0	41.0
16	37.83464619628117	40.0	36.0	41.0	33.0	41.0
17	37.8033404046492	40.0	36.0	41.0	33.0	41.0
18	37.76664444049682	39.0	36.0	41.0	33.0	41.0
19	37.791770455047896	39.0	36.0	41.0	33.0	41.0
20	37.67375590111929	39.0	35.0	41.0	33.0	41.0
21	37.57326643885139	39.0	35.0	41.0	33.0	41.0
22	37.54477339096711	39.0	35.0	41.0	33.0	41.0
23	37.413726445807605	39.0	35.0	41.0	32.0	41.0
24	37.309395404273616	39.0	35.0	41.0	32.0	41.0
25	37.167903108614055	39.0	35.0	41.0	32.0	41.0
26	37.09840117536448	39.0	35.0	41.0	32.0	41.0
27	36.97543503900497	39.0	35.0	41.0	32.0	41.0
28	36.97409292485122	39.0	35.0	41.0	32.0	41.0
29	36.91136279410367	39.0	35.0	41.0	31.0	41.0
30	36.78735571827849	39.0	35.0	40.0	31.0	41.0
31	36.74800115770696	39.0	35.0	40.0	31.0	41.0
32	36.49792933501982	39.0	35.0	41.0	30.0	41.0
33	36.4025018147021	39.0	35.0	41.0	30.0	41.0
34	36.33707357170752	39.0	35.0	41.0	30.0	41.0
35	36.20424627830321	39.0	35.0	41.0	30.0	41.0
36	36.08066853660785	39.0	35.0	41.0	30.0	41.0
37	36.050595567605704	39.0	35.0	41.0	29.0	41.0
38	35.976976512290314	39.0	35.0	40.0	29.0	41.0
39	35.91755253914114	39.0	35.0	40.0	29.0	41.0
40	35.85887153475559	39.0	35.0	40.0	29.0	41.0
41	35.90289252300017	39.0	35.0	40.0	29.0	41.0
42	35.65672813929223	38.0	35.0	40.0	27.0	41.0
43	35.714581447283216	38.0	35.0	40.0	28.0	41.0
44	35.50102687752692	38.0	35.0	40.0	27.0	41.0
45	35.30615047840852	38.0	34.0	40.0	26.0	41.0
46	35.403371518913666	38.0	35.0	40.0	27.0	41.0
47	35.28460118027726	38.0	34.0	40.0	26.0	41.0
48	35.31836442920454	38.0	34.0	40.0	27.0	41.0
49	35.25550471502146	38.0	34.0	40.0	27.0	41.0
50	34.752722409141185	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	5.0
13	6.0
14	15.0
15	31.0
16	110.0
17	308.0
18	788.0
19	1767.0
20	3332.0
21	5628.0
22	8916.0
23	13538.0
24	19554.0
25	28025.0
26	37320.0
27	43298.0
28	47008.0
29	53831.0
30	66292.0
31	84642.0
32	108410.0
33	142763.0
34	213372.0
35	326436.0
36	269056.0
37	395661.0
38	472047.0
39	466835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.51818030680658	25.050792078749705	30.276991713424096	13.154035901019615
2	30.347977804208686	26.35225120959373	30.282260490473305	13.017510495724279
3	27.437619281730406	25.786391674477866	33.1342708671161	13.64171817667562
4	24.309959305817262	27.74046716252504	32.85349489017626	15.096078641481439
5	22.1146236686993	32.53217068986448	31.491053224972156	13.862152416464074
6	19.349761712437978	41.43462391077824	28.196145177591607	11.019469199192168
7	86.87576116918436	3.9143453491116595	7.268562738140713	1.941330743563281
8	53.69136572041092	40.26036302585866	4.028620851327572	2.019650402402847
9	81.51901690316237	5.54040386600076	9.432748510947487	3.5078307198893843
10	42.550251851102935	29.956628708925344	15.741753028923805	11.751366411047913
11	34.7722909319007	23.90219868202254	26.89333325264035	14.432177133436408
12	32.078735465028316	22.722597820363895	28.3791639803617	16.81950273424609
13	21.52288304632145	35.18510673367507	28.642923231426405	14.649086988577078
14	16.75389222004549	37.5201717621318	28.836230389380425	16.889705628442282
15	15.226979271278292	25.460546293860343	43.32882045965808	15.983653975203286
16	16.545170329238047	22.564748107957243	41.625474679432294	19.264606883372416
17	17.426836088701997	23.171654264274025	30.283933683184877	29.117575963839105
18	20.620818575714285	25.03754893643683	34.07820075535751	20.263431732491373
19	25.19497145070436	27.086177612610317	27.695682557606776	20.02316837907854
20	26.69913609856315	25.096039481652017	27.305615056740812	20.899209363044015
21	21.716653002259523	29.60949462104143	28.862645474316313	19.811206902382732
22	21.758732018963325	25.95577573664089	27.00191277966793	25.28357946472785
23	18.291947920274858	30.741391690497792	27.235127363785207	23.731533025442143
24	18.734845591012604	25.918324699777607	36.04694337951464	19.29988632969515
25	17.30348262602968	28.191766396665574	33.33152960785703	21.173221369447713
26	17.259018419715762	32.09197860734119	29.957091506909393	20.691911466033655
27	18.88557533443384	32.04747880118234	29.332385428129076	19.73456043625474
28	16.72651593929657	28.938437544166057	35.73572953516215	18.59931698137523
29	17.350688020402984	25.422561259323157	34.65986662162099	22.566884098652867
30	18.80440768800011	30.072826602767318	30.68984311504339	20.43292259418918
31	24.252501156104962	27.88806411959269	26.197391884160954	21.66204284014139
32	24.564711796115414	27.956593821077313	26.911453573708233	20.567240809099037
33	23.098247384034394	29.88097903845531	25.598602492487544	21.422171085022754
34	18.098569562630985	28.548548042524725	29.11230718678989	24.240575208054395
35	18.113414697965577	29.077027740467166	29.119249156550676	23.690308405016587
36	24.070265549923263	27.69906454287485	27.387459100228163	20.843210806973726
37	18.79141374460173	31.617824272757467	28.45328285749987	21.13747912514093
38	18.568772314427797	31.70728668305921	26.545309168633263	23.17863183387973
39	18.74623754138927	30.531708603877323	27.1780608123671	23.543993042366306
40	21.73128453852455	26.773504174615816	26.898922428293904	24.59628885856573
41	17.75253906993981	25.355419951790694	27.693724566135792	29.198316412133707
42	20.938618391378284	26.717968416529576	26.30899739800733	26.034415794084804
43	20.99910252790939	27.434771294136244	27.4344508955319	24.131675282422467
44	20.19383403565894	30.286995269848603	28.272293245890623	21.246877448601833
45	17.90141762142484	33.047585244718675	26.25250044410807	22.79849668974842
46	21.292765648712834	31.1428155418955	26.507608932855486	21.056809876536178
47	20.320889882203673	27.41889376329877	28.042603046421128	24.21761330807643
48	21.37112090739733	25.288777042087208	29.12736592119405	24.21273612932142
49	21.103018475251524	24.027047338181795	30.248725436552	24.621208750014684
50	19.218469484346926	29.23259906279848	28.690947422233027	22.857984030621562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1489.0
1	1909.0
2	2329.0
3	11648.0
4	20967.0
5	15990.5
6	11014.0
7	11423.5
8	11833.0
9	12858.0
10	13883.0
11	14172.0
12	14461.0
13	14406.5
14	14352.0
15	13810.5
16	13269.0
17	12962.5
18	12656.0
19	12640.5
20	12625.0
21	13657.5
22	14690.0
23	16155.5
24	17621.0
25	20529.0
26	23437.0
27	28606.5
28	33776.0
29	39800.0
30	45824.0
31	52742.0
32	59660.0
33	69042.5
34	78425.0
35	88536.0
36	98647.0
37	110408.0
38	122169.0
39	147439.0
40	172709.0
41	207650.5
42	242592.0
43	261977.0
44	281362.0
45	290275.5
46	299189.0
47	294814.0
48	290439.0
49	270347.5
50	250256.0
51	218514.0
52	186772.0
53	162137.5
54	137503.0
55	119955.5
56	102408.0
57	90784.5
58	79161.0
59	67835.0
60	56509.0
61	46632.0
62	36755.0
63	29372.0
64	21989.0
65	17120.5
66	12252.0
67	9227.0
68	6202.0
69	5221.0
70	4240.0
71	3465.0
72	2690.0
73	2086.0
74	1482.0
75	1092.5
76	703.0
77	528.5
78	354.0
79	257.0
80	160.0
81	125.5
82	91.0
83	60.0
84	29.0
85	20.0
86	11.0
87	8.0
88	5.0
89	5.5
90	6.0
91	4.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2809001.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.87540412902745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.61426318052625	23.963717418754438
2	8.533933929589622	5.269773177729978
3	3.2020134480192017	2.965903777025205
4	1.6550422313499908	2.044003909741533
5	1.037849264899153	1.602200773938771
6	0.757077781449364	1.4025049475613982
7	0.5621615006904036	1.2149874463717731
8	0.44873333072479965	1.1083858345834177
9	0.3922494424629332	1.0899774049885886
>10	4.798862108975727	34.76926030489841
>50	0.8701221895324159	18.111541699001453
>100	0.1250795199491421	5.207802530347867
>500	0.0016169968476970743	0.3365358101153977
>1k	8.706906102984237E-4	0.6259496940626341
>5k	1.2438437289977481E-4	0.2874552708791224
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7485	0.2664648392791601	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4973	0.17703802882234643	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4640	0.1651832804616303	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07045209311068241	0.0
2	0.0	0.0	0.0	0.23079379466223046	0.0
3	0.0	0.0	0.0	0.3491276791998294	0.0
4	0.0	0.0	0.0	0.5112849728426583	0.0
5	0.0	0.0	0.0	0.8517974895701355	0.0
6	0.0	0.0	0.0	1.3477389292492241	0.0
7	0.0	0.0	0.0	1.6977566045722305	0.0
8	0.0	0.0	0.0	2.707119007789602	0.0
9	0.0	0.0	0.0	3.5884643686492104	0.0
10	0.0	0.0	0.0	4.8472748852706	0.0
11	0.0	0.0	0.0	5.56535935729464	0.0
12	0.0	0.0	0.0	6.269275091037703	0.0
13	3.55998449270755E-5	0.0	0.0	6.59957045227111	0.0
14	3.55998449270755E-5	0.0	0.0	6.72993708439406	0.0
15	3.55998449270755E-5	0.0	0.0	6.889851587806484	0.0
16	3.55998449270755E-5	0.0	0.0	7.206334209208185	0.0
17	3.55998449270755E-5	0.0	0.0	7.5731550113367705	0.0
18	3.55998449270755E-5	0.0	0.0	8.142076133116364	0.0
19	3.55998449270755E-5	0.0	0.0	8.381057892111823	0.0
20	1.067995347812265E-4	0.0	0.0	8.658451883783595	0.0
21	5.69597518833208E-4	0.0	0.0	8.948733019318968	0.0
22	5.69597518833208E-4	0.0	0.0	9.23965495206303	0.0
23	5.69597518833208E-4	0.0	0.0	9.584510649871609	0.0
24	5.69597518833208E-4	0.0	0.0	9.836308353040813	0.0
25	5.69597518833208E-4	0.0	0.0	10.05425060368437	0.0
26	5.69597518833208E-4	0.0	0.0	10.262580896197615	0.0
27	5.69597518833208E-4	0.0	0.0	10.486112322494723	0.0
28	6.051973637602834E-4	0.0	0.0	10.707258559181716	0.0
29	6.051973637602834E-4	0.0	0.0	10.951509095226381	0.0
30	6.051973637602834E-4	0.0	0.0	11.261619344386135	0.0
31	6.051973637602834E-4	0.0	0.0	11.52146261250886	0.0
32	6.051973637602834E-4	0.0	0.0	11.761547966697057	0.0
33	6.051973637602834E-4	0.0	0.0	11.995082949418672	0.0
34	6.051973637602834E-4	0.0	0.0	12.239653884067682	0.0
35	6.051973637602834E-4	0.0	0.0	12.521604655890119	0.0
36	6.40797208687359E-4	0.0	0.0	12.779062734402729	0.0
37	6.40797208687359E-4	0.0	0.0	13.037232809813881	0.0
38	6.40797208687359E-4	0.0	0.0	13.296257281503282	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTAC	20	7.858426E-4	44.0	32
CGTTTTT	10855	0.0	42.56103	1
CGACGGT	620	0.0	41.870968	28
GAGCGAT	1755	0.0	41.618237	7
CGGTCTA	635	0.0	40.88189	31
AGCGATA	420	0.0	40.85714	8
CACGACG	650	0.0	39.93846	26
CGTAAGA	225	0.0	39.11111	2
CGTTAGA	130	0.0	38.92308	2
CCAATCG	85	0.0	38.823532	24
CTCACGA	685	0.0	38.540146	24
AACGCGA	80	0.0	38.5	2
TCACGAC	670	0.0	38.41791	25
ACGAGAT	455	0.0	38.197803	5
GACGGTC	685	0.0	37.89781	29
TGCGAAG	205	0.0	37.560978	1
ACGACGG	695	0.0	37.352516	27
CTATGCG	95	0.0	37.052635	1
ACGTTAG	125	0.0	36.96	1
TGCGCGA	125	0.0	36.96	2
>>END_MODULE