FastQCFastQC Report
Thu 26 May 2016
SRR1546768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546768_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2350622
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166950.710237545636857No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100190.4262276112450236No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25700.1093327638386776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT255700.043.363321
TTACGCG650.040.6153831
GTTTTTA115100.039.737622
TACGCGA501.3496901E-939.6000022
GAGCGAT16200.039.1111157
TTCGCGA452.3526809E-839.1111152
ACGAGAT4400.039.05
TAGTACG1650.038.6666681
ACTCCGC404.126996E-738.50000434
CGACGGT2200.038.028
CGTAAGA1400.037.7142872
CGAGATT3950.037.3164566
GTTAGCG1900.037.0526351
GAGACCG4600.036.8260847
AGCGATA3350.036.7761158
CGCGAGT601.9826984E-1036.6666644
TTACGGG4450.036.089893
TATGCGA1100.036.02
AGGGCGA15000.035.4933326
CGAGACC1800.035.4444476
Produced by FastQC (version 0.11.5)