FastQCFastQC Report
Thu 26 May 2016
SRR1546767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546767_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2086167
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140470.6733401496620357No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86120.4128145062212181No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGA207.85796E-444.02
CGCTCGA302.5287136E-644.02
CGTTTTT211250.043.4584661
GAGCGAT14400.042.0138857
GTTTTTA91950.039.5976072
CGAGATT3350.039.4029856
CGACGGT1750.038.97142828
TAGCGCG750.038.133331
TCACGAC1800.037.88888525
CTAACGG357.2930616E-637.7142872
TCGAACG357.2930616E-637.7142871
AGCGATC1350.037.481488
CGGTCTA1850.036.86486431
TCGGGTA301.3014591E-436.6666645
AGAGCGA9200.036.1086966
CTCGTAG553.426976E-936.01
TAAGCGA1100.036.02
GTTTTTT158000.035.7708852
ACGAGAT4000.035.755
GCGATAT2650.035.6981129
Produced by FastQC (version 0.11.5)