##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546767_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2086167
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.476245669689913	31.0	31.0	33.0	30.0	34.0
2	31.87470657909937	31.0	31.0	34.0	30.0	34.0
3	31.956759933408975	31.0	31.0	34.0	30.0	34.0
4	35.68314569255482	37.0	35.0	37.0	33.0	37.0
5	35.576262111326656	37.0	35.0	37.0	33.0	37.0
6	35.62167458309905	37.0	35.0	37.0	33.0	37.0
7	36.02364815472587	37.0	35.0	37.0	35.0	37.0
8	21.981390751555363	35.0	0.0	37.0	0.0	37.0
9	29.88210291889384	37.0	18.0	39.0	17.0	39.0
10	34.29208304033186	35.0	32.0	39.0	27.0	39.0
11	35.957535997837184	37.0	35.0	39.0	32.0	39.0
12	36.57730756933649	38.0	35.0	39.0	32.0	39.0
13	36.781527557477425	39.0	35.0	39.0	33.0	39.0
14	37.96327571090905	40.0	37.0	41.0	33.0	41.0
15	38.158675216317775	40.0	37.0	41.0	33.0	41.0
16	38.16785473070948	40.0	37.0	41.0	33.0	41.0
17	38.11212141693354	40.0	37.0	41.0	33.0	41.0
18	38.02067475901977	40.0	37.0	41.0	33.0	41.0
19	37.97347383982203	40.0	37.0	41.0	33.0	41.0
20	37.822960482070705	40.0	36.0	41.0	33.0	41.0
21	37.738157587575685	40.0	36.0	41.0	33.0	41.0
22	37.71186918401067	40.0	35.0	41.0	33.0	41.0
23	37.52441822730395	39.0	35.0	41.0	33.0	41.0
24	37.333747010665974	39.0	35.0	41.0	33.0	41.0
25	37.20452053934321	39.0	35.0	41.0	32.0	41.0
26	37.143479884400435	39.0	35.0	41.0	32.0	41.0
27	37.02747431054178	39.0	35.0	41.0	32.0	41.0
28	37.01703075544767	39.0	35.0	41.0	32.0	41.0
29	36.91083360056985	39.0	35.0	41.0	32.0	41.0
30	36.77927845661445	39.0	35.0	41.0	31.0	41.0
31	36.60770590273933	39.0	35.0	41.0	31.0	41.0
32	36.17704862554148	39.0	35.0	41.0	30.0	41.0
33	35.81455032123507	39.0	35.0	41.0	27.0	41.0
34	35.526924258700284	39.0	35.0	41.0	24.0	41.0
35	35.24837321269103	39.0	35.0	41.0	23.0	41.0
36	35.094244612248204	39.0	35.0	41.0	22.0	41.0
37	35.053331780245784	39.0	35.0	41.0	22.0	41.0
38	34.94066342723281	39.0	35.0	41.0	21.0	41.0
39	34.82675883570203	39.0	34.0	40.0	20.0	41.0
40	34.75338168037362	39.0	34.0	40.0	20.0	41.0
41	34.72417021264357	38.0	34.0	40.0	19.0	41.0
42	34.46897539842208	38.0	34.0	40.0	18.0	41.0
43	34.52340584430681	38.0	34.0	40.0	18.0	41.0
44	34.34374429276276	38.0	34.0	40.0	18.0	41.0
45	34.17235532917547	38.0	33.0	40.0	18.0	41.0
46	34.25171714440886	38.0	34.0	40.0	18.0	41.0
47	34.13926785343647	38.0	33.0	40.0	18.0	41.0
48	34.13050489246547	38.0	33.0	40.0	18.0	41.0
49	34.041841808445824	38.0	33.0	40.0	18.0	41.0
50	33.58897873468423	37.0	33.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	4.0
14	9.0
15	34.0
16	91.0
17	309.0
18	821.0
19	1888.0
20	3559.0
21	6049.0
22	9623.0
23	14655.0
24	22233.0
25	34121.0
26	46450.0
27	50215.0
28	47674.0
29	45497.0
30	49774.0
31	58604.0
32	73290.0
33	94639.0
34	134573.0
35	192080.0
36	193638.0
37	290245.0
38	345801.0
39	370285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.774461967809863	23.102896364480888	28.321222605860413	18.801419061848836
2	35.17196849533139	24.15055937516028	27.952460181759182	12.725011947749149
3	26.020448027411035	24.13291936839189	37.136480444758256	12.710152159438817
4	22.97548566342004	26.52069561065821	36.75640540762077	13.74741331830098
5	21.35020830067775	30.394354814355708	35.4814355705943	12.774001314372244
6	18.725825880670147	38.323202313141756	32.613784035506264	10.33718777068183
7	82.00781624865124	3.2260121073720365	13.067458166100796	1.6987134778759323
8	50.80341123217844	40.01410241845452	7.61219020337298	1.5702961459940645
9	76.82242121555944	5.353598249804546	14.727152715961859	3.096827818674152
10	33.23899764496323	34.18695626956039	20.884809317758357	11.689236767718022
11	25.937568756480182	24.6797595782121	34.49479356158927	14.887878103718446
12	24.859994429976123	23.20686694785221	35.10438042591988	16.828758196251787
13	20.77110796978382	27.98783606489797	35.60189572550999	15.639160239808222
14	17.682237328075846	30.72884385574117	34.63970046501551	16.949218351167474
15	16.494940242080332	25.312403081824225	42.28443839826821	15.90821827782723
16	18.58748604498106	24.6484102183574	39.788185701336474	16.975918035325073
17	18.99905424637625	23.71075757597546	35.117993909404184	22.172194268244105
18	21.040453616608833	24.15223709319532	36.96142255150235	17.845886738693498
19	22.699237405250873	25.9067466794365	33.34646746880763	18.047548446505
20	23.959922671579026	24.87135497781338	33.14600413102115	18.022718219586448
21	21.440373661360766	26.835099970424224	34.19754986058163	17.52697650763338
22	21.209423790137606	24.234061798504147	33.55306646112224	21.003447950236005
23	18.41650260981024	28.26954889038126	33.46884501576336	19.84510348404514
24	18.065523996880405	25.78331456685874	37.44000360469704	18.71115783156382
25	17.834382386453242	27.665714202170776	35.87406952559407	18.625833885781915
26	18.096825421934103	29.13247117800253	33.82849982767439	18.94220357238898
27	18.559635925599434	27.968805948900545	34.519575853706826	18.951982271793195
28	17.21041508182231	27.35835625815191	37.67445271639327	17.75677594363251
29	18.146102397363205	25.52485011986097	36.64634710452231	19.682700378253514
30	18.818963199015226	27.32341178822213	34.71447875457717	19.143146258185467
31	21.8271595706384	26.62576869445255	31.941354647063253	19.605717087845793
32	21.850503818725922	27.519848602724515	32.0285480500842	18.60109952846536
33	20.775853515082925	28.204213756616802	30.884631958994653	20.135300769305623
34	18.601722680878378	28.469964293366733	32.262182270163414	20.666130755591475
35	19.151678652763657	28.68015839575643	31.262262321281085	20.90590063019883
36	21.89656916248795	28.4459010232642	29.878001137972177	19.779528676275675
37	20.23639526461688	30.59213380328612	29.83049775018011	19.340973181916883
38	20.509048412710968	30.310948260613845	28.991111449850372	20.18889187682482
39	21.193605305807253	29.798477303111397	28.967239918951837	20.04067747212951
40	22.960338266303705	27.63930212681919	29.006498520971714	20.393861085905396
41	20.187837311202795	27.245661540998395	29.551421338751883	23.015079809046927
42	21.6481230888994	28.17559668041916	28.536977145166233	21.639303085515206
43	21.448426707928945	27.509494685708287	29.629842673189632	21.412235933173136
44	20.795219174687357	29.221534038262515	29.80825600251562	20.174990784534508
45	19.432384847425926	30.501153550986093	29.014024284728883	21.052437316859102
46	21.133303326147907	29.54451872740773	28.868638033292637	20.453539913151726
47	20.600412143419007	28.31767543058633	29.921573872082146	21.16033855391251
48	21.544488049135087	26.71128437943846	30.33266272546733	21.41156484595912
49	21.085272655544834	26.003047694647645	30.756214627112787	22.15546502269473
50	19.670812547605248	28.23005061435638	30.660057416304635	21.439079421733734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	890.0
1	1366.5
2	1843.0
3	21584.0
4	41325.0
5	31246.5
6	21168.0
7	21578.0
8	21988.0
9	22594.5
10	23201.0
11	23137.0
12	23073.0
13	22528.0
14	21983.0
15	21114.0
16	20245.0
17	19297.0
18	18349.0
19	17543.5
20	16738.0
21	16760.5
22	16783.0
23	17818.5
24	18854.0
25	20677.0
26	22500.0
27	25998.5
28	29497.0
29	35024.0
30	40551.0
31	46960.5
32	53370.0
33	59931.0
34	66492.0
35	73492.5
36	80493.0
37	88423.0
38	96353.0
39	108131.5
40	119910.0
41	135494.0
42	151078.0
43	162729.5
44	174381.0
45	181337.0
46	188293.0
47	186770.5
48	185248.0
49	174307.5
50	163367.0
51	145870.5
52	128374.0
53	114163.5
54	99953.0
55	88244.5
56	76536.0
57	66824.5
58	57113.0
59	49548.0
60	41983.0
61	34760.0
62	27537.0
63	21798.0
64	16059.0
65	12255.0
66	8451.0
67	6562.5
68	4674.0
69	3783.0
70	2892.0
71	2444.0
72	1996.0
73	1567.5
74	1139.0
75	957.0
76	775.0
77	594.5
78	414.0
79	305.5
80	197.0
81	132.5
82	68.0
83	44.0
84	20.0
85	12.0
86	4.0
87	3.5
88	3.0
89	3.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2086167.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.78666732927035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46256307189236	27.75905431953138
2	7.571513778072283	5.722045446220085
3	3.7581328973188306	4.260219527105207
4	2.592091379378789	3.917859785586233
5	2.0067938893112065	3.7915026546907566
6	1.5728255984557324	3.5659102593484384
7	1.2975839802773579	3.4321961936171714
8	1.055357895412629	3.1902766165820724
9	0.8885197941982436	3.0216781690956704
>10	5.712934394561228	37.13297473600346
>50	0.06612016653608532	1.6038509638401592
>100	0.013939429375883954	1.0130769260368575
>500	0.0010824834728592571	0.2698093350864217
>1k	2.706208682148143E-4	0.16100617702996814
>5k	1.3531043410740714E-4	0.44032556258560995
>10k+	1.3531043410740714E-4	0.7182133276405089
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14047	0.6733401496620357	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8612	0.4128145062212181	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06303426331640756	0.0
2	0.0	0.0	0.0	0.18474072305812525	0.0
3	0.0	0.0	0.0	0.29614120058461285	0.0
4	0.0	0.0	0.0	0.40878798293712826	0.0
5	0.0	0.0	0.0	0.6429974206283582	0.0
6	0.0	0.0	0.0	1.0253733282138966	0.0
7	0.0	0.0	0.0	1.3105374593692642	0.0
8	0.0	0.0	0.0	2.014460012069983	0.0
9	0.0	0.0	0.0	2.735878767136092	0.0
10	0.0	0.0	0.0	3.764032313808051	0.0
11	0.0	0.0	0.0	4.26960065996634	0.0
12	0.0	0.0	0.0	4.8088192364273805	0.0
13	0.0	0.0	0.0	5.103378588578958	0.0
14	0.0	0.0	0.0	5.237116683371945	0.0
15	0.0	0.0	0.0	5.371573800179947	0.0
16	0.0	0.0	0.0	5.606598129488195	0.0
17	0.0	0.0	0.0	5.868945295367054	0.0
18	0.0	0.0	0.0	6.3322830818434	0.0
19	0.0	0.0	0.0	6.5069574966913	0.0
20	0.0	0.0	0.0	6.732346930998333	0.0
21	0.0	0.0	0.0	6.928160593087706	0.0
22	0.0	0.0	0.0	7.136197629432352	0.0
23	0.0	0.0	0.0	7.362258150953399	0.0
24	0.0	0.0	0.0	7.540479741075379	0.0
25	0.0	0.0	0.0	7.693056212661786	0.0
26	0.0	0.0	0.0	7.845249205840185	0.0
27	0.0	0.0	0.0	8.018533511459054	0.0
28	0.0	0.0	0.0	8.181991182872704	0.0
29	0.0	0.0	0.0	8.352255596028506	0.0
30	0.0	0.0	0.0	8.58219883643064	0.0
31	0.0	0.0	0.0	8.77245206160389	0.0
32	0.0	0.0	0.0	8.954220826999947	0.0
33	0.0	0.0	0.0	9.135797853192003	0.0
34	0.0	0.0	0.0	9.31751868378706	0.0
35	0.0	0.0	0.0	9.51405136789145	0.0
36	0.0	0.0	0.0	9.69682676410853	0.0
37	0.0	0.0	0.0	9.89297596980491	0.0
38	0.0	0.0	0.0	10.086536696247233	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGA	20	7.85796E-4	44.0	2
CGCTCGA	30	2.5287136E-6	44.0	2
CGTTTTT	21125	0.0	43.458466	1
GAGCGAT	1440	0.0	42.013885	7
GTTTTTA	9195	0.0	39.597607	2
CGAGATT	335	0.0	39.402985	6
CGACGGT	175	0.0	38.971428	28
TAGCGCG	75	0.0	38.13333	1
TCACGAC	180	0.0	37.888885	25
CTAACGG	35	7.2930616E-6	37.714287	2
TCGAACG	35	7.2930616E-6	37.714287	1
AGCGATC	135	0.0	37.48148	8
CGGTCTA	185	0.0	36.864864	31
TCGGGTA	30	1.3014591E-4	36.666664	5
AGAGCGA	920	0.0	36.108696	6
CTCGTAG	55	3.426976E-9	36.0	1
TAAGCGA	110	0.0	36.0	2
GTTTTTT	15800	0.0	35.770885	2
ACGAGAT	400	0.0	35.75	5
GCGATAT	265	0.0	35.698112	9
>>END_MODULE