FastQCFastQC Report
Thu 26 May 2016
SRR1546766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546766_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1487225
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA51660.3473583351543983No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30670.20622299920993797No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.12089630015633143No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC15560.10462438433996202No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAA207.85723E-444.017
GGTACGA207.85723E-444.02
TCCGCGA207.85723E-444.02
CGGTTCG207.85723E-444.029
CGCATCG600.044.021
GCACGTC302.5282206E-644.044
TCGATTG302.5282206E-644.01
CGTTCCG207.85723E-444.024
CGCTCAC207.85723E-444.031
CGAGGTA351.4464604E-744.06
GTTCCCG207.85723E-444.037
CTCGCTA302.5282206E-644.012
TTACGTA207.85723E-444.020
ACGTAAG454.802132E-1044.01
ACCCCGT207.85723E-444.022
TACGTAG254.443474E-544.01
TACGTAC207.85723E-444.01
GTTGCGA700.044.02
CTCACGA4600.042.56521624
CGACGGT4500.042.53333328
Produced by FastQC (version 0.11.5)