##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546766_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1487225
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444513775656002	31.0	31.0	33.0	30.0	34.0
2	31.822539292978533	31.0	31.0	34.0	30.0	34.0
3	31.97120745011683	31.0	31.0	34.0	30.0	34.0
4	35.67628099312478	37.0	35.0	37.0	33.0	37.0
5	35.550412345139435	37.0	35.0	37.0	33.0	37.0
6	35.60812990636924	37.0	35.0	37.0	33.0	37.0
7	35.949468305064805	37.0	35.0	37.0	35.0	37.0
8	21.9214483349863	35.0	0.0	37.0	0.0	37.0
9	29.857439862831782	37.0	18.0	39.0	17.0	39.0
10	34.25287565768461	35.0	32.0	39.0	27.0	39.0
11	35.91893291196692	37.0	35.0	39.0	32.0	39.0
12	36.140019163206645	37.0	35.0	39.0	32.0	39.0
13	36.13153423321959	37.0	35.0	39.0	32.0	39.0
14	37.18548908201516	39.0	35.0	41.0	32.0	41.0
15	37.51616534149171	39.0	35.0	41.0	33.0	41.0
16	37.61384390391501	39.0	35.0	41.0	33.0	41.0
17	37.600083376758725	39.0	35.0	41.0	33.0	41.0
18	37.57752794634302	39.0	36.0	41.0	33.0	41.0
19	37.57787557363546	39.0	36.0	41.0	33.0	41.0
20	37.44319386777387	39.0	35.0	41.0	33.0	41.0
21	37.3375615660038	39.0	35.0	41.0	33.0	41.0
22	37.30006623073173	39.0	35.0	41.0	32.0	41.0
23	37.193481147775216	39.0	35.0	41.0	32.0	41.0
24	37.112114844761216	39.0	35.0	41.0	32.0	41.0
25	36.958972583166634	38.0	35.0	41.0	32.0	41.0
26	36.90869572526013	38.0	35.0	41.0	32.0	41.0
27	36.76164467380524	38.0	35.0	40.0	32.0	41.0
28	36.81169964195061	38.0	35.0	40.0	32.0	41.0
29	36.717220326446906	38.0	35.0	40.0	31.0	41.0
30	36.656656524735666	38.0	35.0	40.0	31.0	41.0
31	36.55230849400729	38.0	35.0	40.0	31.0	41.0
32	36.27946343021399	38.0	35.0	40.0	30.0	41.0
33	36.14961690396544	38.0	35.0	40.0	30.0	41.0
34	36.092458101497755	38.0	35.0	40.0	30.0	41.0
35	35.92803375413942	38.0	35.0	40.0	30.0	41.0
36	35.77075291230312	38.0	35.0	40.0	29.0	41.0
37	35.696210728033755	38.0	35.0	40.0	29.0	41.0
38	35.637307737564925	38.0	35.0	40.0	29.0	41.0
39	35.59103632604347	38.0	35.0	40.0	29.0	41.0
40	35.50037351443124	38.0	35.0	40.0	28.0	41.0
41	35.557628469128744	38.0	35.0	40.0	29.0	41.0
42	35.33242717140984	38.0	35.0	40.0	27.0	41.0
43	35.3498831716788	38.0	35.0	40.0	28.0	41.0
44	35.14064179932424	38.0	34.0	40.0	27.0	41.0
45	34.923327001630554	37.0	34.0	40.0	26.0	41.0
46	35.01010001849081	37.0	34.0	40.0	27.0	41.0
47	34.87510430499756	37.0	34.0	40.0	26.0	41.0
48	34.91417035082116	37.0	34.0	40.0	26.0	41.0
49	34.86273294222461	37.0	34.0	40.0	26.0	41.0
50	34.33677083158231	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	6.0
15	31.0
16	75.0
17	175.0
18	424.0
19	926.0
20	1831.0
21	3055.0
22	5040.0
23	7540.0
24	10996.0
25	14998.0
26	19318.0
27	22388.0
28	25137.0
29	29089.0
30	35723.0
31	45520.0
32	59890.0
33	79805.0
34	130378.0
35	216206.0
36	139596.0
37	199569.0
38	236913.0
39	202587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.75758543596295	24.57711509690867	30.26092218729513	12.404377279833247
2	29.21629208761284	26.985963791625345	30.695826119114457	13.101918001647364
3	28.137100976651148	25.459698431642824	32.81870597925667	13.58449461244936
4	24.78105195918573	28.721814116895562	31.777034409722805	14.720099514195903
5	22.515423019381732	32.353342634772815	31.5449242717141	13.586310074131353
6	19.315907142496933	41.94583872648725	27.43653448536704	11.301719645648776
7	86.31800837129553	5.058817596530451	6.738455848980483	1.8847181831935318
8	53.75044125804771	40.63218410126242	3.6246028677570643	1.992771772932811
9	83.09576560372506	5.377531980702314	8.452722352031468	3.0739800635411587
10	50.65111197028022	23.965842424649935	13.84854342819681	11.534502176873035
11	44.55795189026543	22.017583082586697	20.171796466573653	13.252668560574223
12	39.791423624535625	21.763687404394087	24.20306275109684	14.241826219973442
13	22.29756761754274	41.09431995831162	24.19149758778934	12.416614836356302
14	15.468540402427339	44.117870530686346	26.357814049656238	14.055775017230076
15	13.525391248802299	23.458454504194055	48.947872715964294	14.06828153103935
16	13.971793104607574	20.34984619005194	46.258568811040696	19.419791894299788
17	15.369900317705795	20.73499302392039	28.267343542503653	35.62776311587016
18	21.553093849283062	22.966060952444987	34.20834103783893	21.27250416043302
19	28.14207668644623	25.89776261157525	25.364487552320597	20.59567314965792
20	29.907714031165426	24.076854544537646	24.584242464993526	21.4311889593034
21	20.855956563398276	30.888534014691793	27.359679940829395	20.895829481080536
22	21.608835246852358	26.280488829867704	24.99816772848762	27.112508194792312
23	18.315318798433324	32.64105969170771	24.289801475903108	24.753820033955858
24	19.05703575450924	25.420901343105445	36.99238514683387	18.529677755551447
25	15.82195027652171	26.959337020289464	35.28195128511153	21.936761418077293
26	16.185916724100256	35.15117080468658	27.492477600901005	21.170434870312157
27	17.694767099799964	34.419674225487064	27.960597757568628	19.924960917144347
28	15.06349072937854	29.27660575904789	38.022894988989556	17.637008522584008
29	15.54122610902856	25.815932357242517	36.721141723680006	21.921699810048917
30	18.559868210929753	31.316176099783156	30.1637613676478	19.960194321639293
31	28.517810015296945	27.26137605271563	23.644841903545196	20.575972028442234
32	29.034140765519677	28.40740304930323	24.835852006253255	17.722604178923834
33	27.685588932407672	28.397182672426837	24.65558338516364	19.26164501000185
34	20.170048244213216	28.841096673334565	27.275294592277564	23.713560490174654
35	20.682680831750407	27.062549378876767	29.896283346501036	22.35848644287179
36	29.995663063759686	26.380271983055692	25.439593874497806	18.184471078686816
37	22.08119148077796	32.00181546168199	27.871572895829484	18.045420161710567
38	20.384071004723562	34.210358217485584	23.894299786515155	21.5112709912757
39	21.07011380255173	31.817781438585286	26.06478508631848	21.047319672544504
40	23.850392509539578	27.138395333591088	24.32483316243339	24.686378994435945
41	17.45028492662509	25.15315436467246	25.446183328010218	31.95037738069223
42	22.039704819378372	27.05508581418413	23.594479651700315	27.31072971473718
43	22.41281581468843	26.42491889256837	25.99075459328615	25.171510699457045
44	20.397249911748393	30.56598698919128	27.800702650910253	21.23606044815008
45	17.649246079107062	35.89006370925718	23.4968481568021	22.963842054833666
46	22.576610801997006	32.62135857049203	25.292272520970265	19.509758106540705
47	21.992973490897477	27.116340836120962	26.63060397720587	24.26008169577569
48	23.288876935231723	24.426700734589588	29.70848392139723	22.575938408781454
49	22.058599068735397	22.5951016154247	31.01413706735699	24.332162248482913
50	19.420598766158452	31.41212661164249	26.594899897460035	22.572374724739028
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1503.0
1	1624.5
2	1746.0
3	5242.5
4	8739.0
5	6934.0
6	5129.0
7	5740.5
8	6352.0
9	7057.5
10	7763.0
11	7993.0
12	8223.0
13	8129.0
14	8035.0
15	7702.0
16	7369.0
17	7077.5
18	6786.0
19	6621.0
20	6456.0
21	6248.0
22	6040.0
23	6441.0
24	6842.0
25	7868.5
26	8895.0
27	10485.0
28	12075.0
29	14340.0
30	16605.0
31	19938.0
32	23271.0
33	26144.5
34	29018.0
35	33523.0
36	38028.0
37	46243.5
38	54459.0
39	73528.5
40	92598.0
41	115851.5
42	139105.0
43	152110.5
44	165116.0
45	165060.0
46	165004.0
47	158282.0
48	151560.0
49	143324.5
50	135089.0
51	119189.0
52	103289.0
53	89453.5
54	75618.0
55	64470.0
56	53322.0
57	47946.5
58	42571.0
59	38693.0
60	34815.0
61	30616.5
62	26418.0
63	21246.5
64	16075.0
65	12554.0
66	9033.0
67	7335.0
68	5637.0
69	4679.0
70	3721.0
71	2926.0
72	2131.0
73	1698.0
74	1265.0
75	1023.5
76	782.0
77	572.5
78	363.0
79	304.0
80	245.0
81	165.5
82	86.0
83	57.0
84	28.0
85	18.5
86	9.0
87	7.0
88	5.0
89	5.5
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1487225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.878439082064745
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.00096746659493	23.305471547927706
2	7.328077423512203	4.3790302977412665
3	2.743226964745411	2.458900192632694
4	1.5996427715687878	1.9117931641353298
5	1.0802458369603831	1.6138029716637428
6	0.8052939805859421	1.4436556285254303
7	0.6383259182248389	1.3350527443528724
8	0.5374552180827742	1.2846658394259194
9	0.44992869595321605	1.2098850419979899
>10	5.938021296668386	41.0842439662777
>50	0.8088026593901806	15.858497359257598
>100	0.06727515157795358	2.912907437990774
>500	0.0015963594121393189	0.3383742121573876
>1k	9.12205378365325E-4	0.511717747342324
>5k	2.2805134459133125E-4	0.3520018485712977
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5166	0.3473583351543983	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3067	0.20622299920993797	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1798	0.12089630015633143	No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1556	0.10462438433996202	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11511371850258031	0.0
2	0.0	0.0	0.0	0.30560271646859083	0.0
3	0.0	0.0	0.0	0.4295247860949083	0.0
4	0.0	0.0	0.0	0.5867975592126276	0.0
5	0.0	0.0	0.0	0.8987207719074115	0.0
6	0.0	0.0	0.0	1.328043840037654	0.0
7	0.0	0.0	0.0	1.6091714434601354	0.0
8	0.0	0.0	0.0	2.41012624182622	0.0
9	0.0	0.0	0.0	3.0946225352586194	0.0
10	0.0	0.0	0.0	4.233118727832037	0.0
11	0.0	0.0	0.0	5.07623258081326	0.0
12	0.0	0.0	0.0	5.882701003546874	0.0
13	0.0	0.0	0.0	6.261729059153793	0.0
14	0.0	0.0	0.0	6.424179260031266	0.0
15	0.0	0.0	0.0	6.619576728470809	0.0
16	0.0	0.0	0.0	7.030610701138025	0.0
17	0.0	0.0	0.0	7.507942644858713	0.0
18	0.0	0.0	0.0	8.292625527408429	0.0
19	0.0	0.0	0.0	8.576509943014676	0.0
20	0.0	0.0	0.0	8.886953890635244	0.0
21	0.0	0.0	0.0	9.228462404814335	0.0
22	0.0	0.0	0.0	9.564995209198338	0.0
23	0.0	0.0	0.0	9.963993343307166	0.0
24	0.0	0.0	0.0	10.2432382457261	0.0
25	0.0	0.0	0.0	10.47998789692212	0.0
26	0.0	0.0	0.0	10.709879137319504	0.0
27	0.0	0.0	0.0	10.99040158684799	0.0
28	0.0	0.0	0.0	11.242750760644824	0.0
29	0.0	0.0	0.0	11.511035653650255	0.0
30	0.0	0.0	0.0	11.831296542217888	0.0
31	0.0	0.0	0.0	12.134142446502715	0.0
32	0.0	0.0	0.0	12.385684748440887	0.0
33	0.0	0.0	0.0	12.637092571735952	0.0
34	0.0	0.0	0.0	12.899393165122964	0.0
35	0.0	0.0	0.0	13.206138950057994	0.0
36	0.0	0.0	0.0	13.47644102271008	0.0
37	0.0	0.0	0.0	13.73793474423843	0.0
38	0.0	0.0	0.0	14.01146430432517	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAA	20	7.85723E-4	44.0	17
GGTACGA	20	7.85723E-4	44.0	2
TCCGCGA	20	7.85723E-4	44.0	2
CGGTTCG	20	7.85723E-4	44.0	29
CGCATCG	60	0.0	44.0	21
GCACGTC	30	2.5282206E-6	44.0	44
TCGATTG	30	2.5282206E-6	44.0	1
CGTTCCG	20	7.85723E-4	44.0	24
CGCTCAC	20	7.85723E-4	44.0	31
CGAGGTA	35	1.4464604E-7	44.0	6
GTTCCCG	20	7.85723E-4	44.0	37
CTCGCTA	30	2.5282206E-6	44.0	12
TTACGTA	20	7.85723E-4	44.0	20
ACGTAAG	45	4.802132E-10	44.0	1
ACCCCGT	20	7.85723E-4	44.0	22
TACGTAG	25	4.443474E-5	44.0	1
TACGTAC	20	7.85723E-4	44.0	1
GTTGCGA	70	0.0	44.0	2
CTCACGA	460	0.0	42.565216	24
CGACGGT	450	0.0	42.533333	28
>>END_MODULE