FastQCFastQC Report
Thu 26 May 2016
SRR1546765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546765_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3734833
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225720.6043643718474159No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136530.36555851359351277No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA39490.10573431261852939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT343600.043.4365581
GAGCGAT24950.042.2364737
CGACGGT5650.040.88495628
AGCGATA5500.040.48
GTCGCGA557.8216544E-1140.02
TTTCGCG1050.039.809521
GTTTTTA155100.039.645392
CGCGCGA501.3496901E-939.62
TCCGCGA452.3534085E-839.1111072
TATGCGA1750.038.971432
ACGGTCT5950.038.8235330
CGCGAGT800.038.5000044
ACGTCGC3550.038.42253520
TGCGCGA1150.038.260872
GAGACCG7900.038.15197
CGGTCTA6100.037.86885531
GCGTTAG1950.037.2307661
CGAGGTA1950.037.2307666
GCGATAC2250.037.1555569
AGCGATC4000.036.858
Produced by FastQC (version 0.11.5)