##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546765_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3734833
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.420746791088117	31.0	31.0	33.0	30.0	34.0
2	31.812419993075995	31.0	31.0	34.0	30.0	34.0
3	31.901238422173094	31.0	31.0	34.0	30.0	34.0
4	35.63210510349459	37.0	35.0	37.0	33.0	37.0
5	35.52398648078776	37.0	35.0	37.0	33.0	37.0
6	35.57514325272375	37.0	35.0	37.0	33.0	37.0
7	35.99518559464372	37.0	35.0	37.0	35.0	37.0
8	22.044465977461375	35.0	0.0	37.0	0.0	37.0
9	29.90591172349607	37.0	18.0	39.0	17.0	39.0
10	34.216874221685416	35.0	32.0	39.0	27.0	39.0
11	35.86405684002471	37.0	35.0	39.0	32.0	39.0
12	36.48192462688426	37.0	35.0	39.0	32.0	39.0
13	36.691592903886196	39.0	35.0	39.0	33.0	39.0
14	37.86177641677687	40.0	37.0	41.0	33.0	41.0
15	38.074171455591184	40.0	37.0	41.0	33.0	41.0
16	38.09606159097341	40.0	37.0	41.0	33.0	41.0
17	38.03980981211208	40.0	37.0	41.0	33.0	41.0
18	37.93844115653899	39.0	36.0	41.0	33.0	41.0
19	37.924583776570465	40.0	37.0	41.0	33.0	41.0
20	37.77023765185753	40.0	36.0	41.0	33.0	41.0
21	37.688411503272036	39.0	36.0	41.0	33.0	41.0
22	37.65679563182611	39.0	35.0	41.0	33.0	41.0
23	37.4743620397485	39.0	35.0	41.0	33.0	41.0
24	37.30807107037985	39.0	35.0	41.0	32.0	41.0
25	37.176537746132155	39.0	35.0	41.0	32.0	41.0
26	37.118211978955955	39.0	35.0	41.0	32.0	41.0
27	37.00454960101295	39.0	35.0	41.0	32.0	41.0
28	36.983861393534866	39.0	35.0	41.0	32.0	41.0
29	36.88273264159334	39.0	35.0	41.0	31.0	41.0
30	36.73537451339859	39.0	35.0	41.0	31.0	41.0
31	36.59157343849109	39.0	35.0	41.0	31.0	41.0
32	36.16875854957906	39.0	35.0	41.0	30.0	41.0
33	35.848714788586264	39.0	35.0	41.0	27.0	41.0
34	35.591895808996014	39.0	35.0	41.0	25.0	41.0
35	35.27784026755681	39.0	35.0	41.0	24.0	41.0
36	35.14968781736693	39.0	35.0	41.0	23.0	41.0
37	35.10764068969081	39.0	35.0	40.0	23.0	41.0
38	35.02980026148425	39.0	35.0	40.0	23.0	41.0
39	34.924500238698755	38.0	34.0	40.0	22.0	41.0
40	34.91478414162025	38.0	34.0	40.0	22.0	41.0
41	34.92789690998232	39.0	35.0	40.0	22.0	41.0
42	34.693922860807966	38.0	34.0	40.0	20.0	41.0
43	34.75789573456163	38.0	34.0	40.0	21.0	41.0
44	34.569742475767995	38.0	34.0	40.0	20.0	41.0
45	34.37659649039194	38.0	34.0	40.0	19.0	41.0
46	34.45438095893444	38.0	34.0	40.0	20.0	41.0
47	34.321564578657195	38.0	34.0	40.0	19.0	41.0
48	34.33581340852456	38.0	34.0	40.0	20.0	41.0
49	34.254013499398766	38.0	34.0	40.0	19.0	41.0
50	33.79848202048124	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	4.0
13	16.0
14	14.0
15	62.0
16	183.0
17	502.0
18	1363.0
19	3256.0
20	6263.0
21	10612.0
22	16710.0
23	25530.0
24	38379.0
25	58229.0
26	78509.0
27	84308.0
28	79826.0
29	79947.0
30	89373.0
31	108793.0
32	136791.0
33	175889.0
34	249689.0
35	354246.0
36	351681.0
37	520123.0
38	611732.0
39	652797.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.379243195077265	23.383481938817614	28.490858895163452	17.746415970941673
2	34.775504018519705	24.57159396417457	28.3026844841523	12.350217533153424
3	25.967131595977648	24.307539319696488	36.954369847326504	12.770959236999351
4	22.87296379784585	26.357296296782213	36.869894852058984	13.899845053312958
5	21.279130820574842	30.440263326365596	35.298686715041875	12.981919138017684
6	18.39147828028723	39.04795743209937	32.308218332653695	10.252345954959699
7	82.33390890569939	3.3007633808526378	12.436432900748173	1.9288948126997911
8	51.00910803776233	39.832785026800394	7.282172991402828	1.8759339440344456
9	76.99696345191339	5.394350965625504	14.307547352184155	3.3011382302769627
10	35.19319337705327	32.47210785596036	21.050472671736596	11.284226095249775
11	26.403563425727466	25.161874707650917	33.59502285644365	14.839539010177965
12	24.891019223617228	22.56041970283544	35.10298854058535	17.445572532961982
13	20.159910764417045	28.246831919927878	36.054784778864274	15.5384725367908
14	17.35908941577843	31.405473819043582	34.271465417596986	16.963971347581
15	15.897176660910944	25.15151279856422	43.00824160009296	15.943068940431877
16	17.44768775471353	24.197119389273897	40.53308407631613	17.822108779696443
17	18.568835607910717	24.5107612575984	34.59375559763984	22.326647536851045
18	20.01794993243339	24.632614095462905	36.74640874170277	18.603027230400933
19	22.331172504901826	26.94514051900045	32.36163437562001	18.362052600477718
20	23.315259343590462	25.546095367584037	32.339384384790435	18.799260904035066
21	21.166006619305332	27.576842123864708	33.37683371652762	17.880317540302336
22	20.12882503715695	24.830132967123294	32.95828755930988	22.082754436409875
23	17.72135460942966	28.504246374603632	32.98235824734333	20.79204076862339
24	18.066992553616185	25.827660835169873	37.36887298575331	18.73647362546063
25	17.49261613571477	27.622225679166913	35.35475347893735	19.530404706180974
26	17.635755065889157	29.392532410418347	33.682389547270255	19.289322976422238
27	18.586159006306303	28.303032558617748	33.725416906190986	19.38539152888496
28	17.540141687727402	27.29026973896825	37.07274194053657	18.096846632767782
29	18.470651833696447	24.746916394923147	36.00110098630916	20.781330785071248
30	19.20626705397537	26.970737379690068	33.659389857592025	20.163605708742534
31	21.83414894320576	26.09155482989467	30.992362978478553	21.081933248421013
32	22.799359435883744	26.187114658138665	31.00261778772973	20.01090811824786
33	21.499087107776973	26.505067294842906	30.84089703609238	21.15494856128775
34	20.416655845120786	26.328379341191425	31.47120634309486	21.78375847059293
35	19.7545914368862	26.134314439226603	32.51044959707703	21.600644526810168
36	23.573664471744788	26.773298832906317	30.388025381590023	19.265011313758876
37	20.077631315777708	28.383410985176578	33.021503237226405	18.51745446181931
38	20.41839621744801	29.92339952013919	30.228446626663093	19.429757635749713
39	19.937223431409116	28.742650608474328	32.03163300741961	19.28849295269695
40	21.186516237807687	27.220253221496115	31.045564821773823	20.547665718922374
41	18.32178306232166	26.524184615483478	31.747764893370068	23.406267428824794
42	20.475319780027647	27.746247288700726	29.605821732859273	22.17261119841235
43	20.50680713167095	27.096231611962303	30.680247282810235	21.716713973556516
44	20.702478531168595	28.970425183669523	29.996789682430247	20.330306602731635
45	18.962614928164122	29.689788003908074	30.104746316635843	21.242850751291957
46	21.477372616125002	29.344605234022513	29.178011439868932	20.000010709983552
47	20.241012114865644	27.088172349339313	30.448590338577386	22.222225197217654
48	20.810301290579794	26.338848350113647	30.593094791654675	22.257755567651888
49	20.83860242211633	25.039673795320972	31.517393147163475	22.60433063539923
50	19.48220978019633	27.323363588144367	31.19250579610922	22.001920835550077
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1627.0
1	2486.5
2	3346.0
3	34419.0
4	65492.0
5	49529.0
6	33566.0
7	34335.5
8	35105.0
9	36335.5
10	37566.0
11	37883.0
12	38200.0
13	37289.0
14	36378.0
15	34533.5
16	32689.0
17	31157.5
18	29626.0
19	28466.0
20	27306.0
21	27836.0
22	28366.0
23	30042.0
24	31718.0
25	35875.0
26	40032.0
27	47072.5
28	54113.0
29	63456.5
30	72800.0
31	83773.0
32	94746.0
33	108391.0
34	122036.0
35	135072.0
36	148108.0
37	162882.0
38	177656.0
39	200274.5
40	222893.0
41	250537.0
42	278181.0
43	297612.0
44	317043.0
45	333398.0
46	349753.0
47	352251.0
48	354749.0
49	332863.5
50	310978.0
51	271388.0
52	231798.0
53	198375.5
54	164953.0
55	145353.0
56	125753.0
57	111642.0
58	97531.0
59	82735.0
60	67939.0
61	56365.0
62	44791.0
63	35953.5
64	27116.0
65	20693.5
66	14271.0
67	10677.5
68	7084.0
69	5569.0
70	4054.0
71	3214.5
72	2375.0
73	1908.0
74	1441.0
75	1085.5
76	730.0
77	577.0
78	424.0
79	345.5
80	267.0
81	218.5
82	170.0
83	99.0
84	28.0
85	22.5
86	17.0
87	12.0
88	7.0
89	7.0
90	7.0
91	4.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3734833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.759068900764724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.36203440014728	27.237913529188152
2	7.178209419471432	4.990157515910517
3	2.8224554523286214	2.9431777061048883
4	1.6841525550466283	2.3415829880105883
5	1.2141800268730891	2.110188360600703
6	0.9180757012083836	1.9146873932652055
7	0.7603499380600436	1.8500339120004872
8	0.6259323323335763	1.740546005343931
9	0.5609560931699779	1.7548480343519148
>10	5.398912493734512	39.00416196892061
>50	0.43552760294006937	9.888892537274558
>100	0.03591792140314318	1.9343370591486715
>500	0.0020489042031025147	0.5076295351220621
>1k	0.0010689934972057944	0.6601599175503242
>5k	0.0	0.0
>10k+	1.781655828676324E-4	1.1216835372073404
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22572	0.6043643718474159	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13653	0.36555851359351277	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3949	0.10573431261852939	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.67749588803569E-5	0.0	0.0	0.08669731685459564	0.0
2	2.67749588803569E-5	0.0	0.0	0.2495426167649263	0.0
3	2.67749588803569E-5	0.0	0.0	0.38194478842829116	0.0
4	2.67749588803569E-5	0.0	0.0	0.5317239084050077	0.0
5	2.67749588803569E-5	0.0	0.0	0.8545763625843512	0.0
6	2.67749588803569E-5	0.0	0.0	1.3758580370260196	0.0
7	2.67749588803569E-5	0.0	0.0	1.7634791167369466	0.0
8	1.338747944017845E-4	0.0	0.0	2.720683896709706	0.0
9	1.338747944017845E-4	0.0	0.0	3.6016603687500885	0.0
10	1.338747944017845E-4	0.0	0.0	4.846695956686685	0.0
11	1.338747944017845E-4	0.0	0.0	5.474916816896498	0.0
12	1.338747944017845E-4	0.0	0.0	6.117114205641859	0.0
13	1.338747944017845E-4	0.0	0.0	6.447490423266583	0.0
14	1.338747944017845E-4	0.0	0.0	6.608354376219767	0.0
15	1.338747944017845E-4	0.0	0.0	6.758320920908646	0.0
16	1.338747944017845E-4	0.0	0.0	7.029256729818977	0.0
17	1.338747944017845E-4	0.0	0.0	7.327502996787273	0.0
18	1.338747944017845E-4	0.0	0.0	7.831327398038948	0.0
19	1.338747944017845E-4	0.0	0.0	8.04665161735478	0.0
20	1.338747944017845E-4	0.0	0.0	8.303477022935162	0.0
21	1.338747944017845E-4	0.0	0.0	8.543889378721886	0.0
22	1.338747944017845E-4	0.0	0.0	8.809550520732788	0.0
23	1.338747944017845E-4	0.0	0.0	9.107743237783323	0.0
24	1.338747944017845E-4	0.0	0.0	9.339159207386247	0.0
25	1.338747944017845E-4	0.0	0.0	9.53820425170282	0.0
26	1.338747944017845E-4	0.0	0.0	9.731385580024595	0.0
27	1.338747944017845E-4	0.0	0.0	9.95452808733349	0.0
28	1.338747944017845E-4	0.0	0.0	10.17060200549797	0.0
29	1.338747944017845E-4	0.0	0.0	10.40161635071769	0.0
30	1.338747944017845E-4	0.0	0.0	10.707841555432331	0.0
31	1.338747944017845E-4	0.0	0.0	10.951466906284699	0.0
32	1.338747944017845E-4	0.0	0.0	11.186176195829907	0.0
33	1.338747944017845E-4	0.0	0.0	11.426481451781111	0.0
34	1.338747944017845E-4	0.0	0.0	11.655487675084803	0.0
35	1.338747944017845E-4	0.0	0.0	11.914481852334495	0.0
36	1.338747944017845E-4	0.0	0.0	12.161587947841308	0.0
37	1.338747944017845E-4	0.0	0.0	12.40625752209001	0.0
38	1.338747944017845E-4	0.0	0.0	12.656228538197022	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	34360	0.0	43.436558	1
GAGCGAT	2495	0.0	42.236473	7
CGACGGT	565	0.0	40.884956	28
AGCGATA	550	0.0	40.4	8
GTCGCGA	55	7.8216544E-11	40.0	2
TTTCGCG	105	0.0	39.80952	1
GTTTTTA	15510	0.0	39.64539	2
CGCGCGA	50	1.3496901E-9	39.6	2
TCCGCGA	45	2.3534085E-8	39.111107	2
TATGCGA	175	0.0	38.97143	2
ACGGTCT	595	0.0	38.82353	30
CGCGAGT	80	0.0	38.500004	4
ACGTCGC	355	0.0	38.422535	20
TGCGCGA	115	0.0	38.26087	2
GAGACCG	790	0.0	38.1519	7
CGGTCTA	610	0.0	37.868855	31
GCGTTAG	195	0.0	37.230766	1
CGAGGTA	195	0.0	37.230766	6
GCGATAC	225	0.0	37.155556	9
AGCGATC	400	0.0	36.85	8
>>END_MODULE