FastQCFastQC Report
Thu 26 May 2016
SRR1546764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546764_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4779813
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136820.28624550793095876No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84570.1769316079938692No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA77470.1620774703947623No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA207.8589807E-444.015
CGTTTTT186050.042.6046751
AGCGATA7000.038.6571438
CGACGGT2450.038.61224428
GAGCGAT36500.038.394527
AGACCGA11450.038.235818
CGCTCGA357.295035E-637.7142872
GACCGAT28000.037.635719
ACGAGAC6050.036.7272725
GTTGCGA2050.036.48782
GAGACCG13000.036.3846137
CGGTCTA2300.036.34782831
CTACGCG850.036.235291
CTTGCGA2800.036.1428572
AATCGCG1400.036.1428571
TTAGCGA3350.036.1194042
CGAGGTT7150.036.06
CCCGGTA553.428795E-936.021
GTTAGCG2450.035.9183651
CACAACG14400.035.7512
Produced by FastQC (version 0.11.5)