##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546764_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4779813
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.492113812820712	31.0	31.0	33.0	30.0	34.0
2	31.885860806688463	31.0	31.0	34.0	30.0	34.0
3	32.026112946259616	33.0	31.0	34.0	30.0	34.0
4	35.71379277808567	37.0	35.0	37.0	35.0	37.0
5	35.601883588332846	37.0	35.0	37.0	33.0	37.0
6	35.657129055048806	37.0	35.0	37.0	33.0	37.0
7	36.03981850335986	37.0	35.0	37.0	35.0	37.0
8	21.89347491209384	35.0	0.0	37.0	0.0	37.0
9	29.85569560984917	37.0	18.0	39.0	17.0	39.0
10	34.3315901689041	35.0	32.0	39.0	27.0	39.0
11	35.928557246904845	37.0	35.0	39.0	32.0	39.0
12	36.44540842915821	37.0	35.0	39.0	32.0	39.0
13	36.59938118918041	39.0	35.0	39.0	33.0	39.0
14	37.74027268430794	39.0	36.0	41.0	33.0	41.0
15	37.973818013382534	40.0	36.0	41.0	33.0	41.0
16	38.01640775486405	40.0	36.0	41.0	33.0	41.0
17	37.96382013271231	40.0	36.0	41.0	33.0	41.0
18	37.92688605181834	40.0	36.0	41.0	33.0	41.0
19	37.9497886214377	40.0	36.0	41.0	33.0	41.0
20	37.86029725430681	40.0	36.0	41.0	33.0	41.0
21	37.75910814921002	40.0	36.0	41.0	33.0	41.0
22	37.72796529906086	40.0	36.0	41.0	33.0	41.0
23	37.59160075927657	39.0	35.0	41.0	33.0	41.0
24	37.49281802447083	39.0	35.0	41.0	33.0	41.0
25	37.367482158820856	39.0	35.0	41.0	32.0	41.0
26	37.32442126920028	39.0	35.0	41.0	32.0	41.0
27	37.21103838162706	39.0	35.0	41.0	32.0	41.0
28	37.219084512302054	39.0	35.0	41.0	32.0	41.0
29	37.14228652878261	39.0	35.0	41.0	32.0	41.0
30	37.0344605531639	39.0	35.0	41.0	32.0	41.0
31	36.9846324531943	39.0	35.0	41.0	31.0	41.0
32	36.756924590982955	39.0	35.0	41.0	31.0	41.0
33	36.64290192942695	39.0	35.0	41.0	30.0	41.0
34	36.57734790043042	39.0	35.0	41.0	30.0	41.0
35	36.43114636493101	39.0	35.0	41.0	30.0	41.0
36	36.34206965837367	39.0	35.0	41.0	30.0	41.0
37	36.32591965417894	39.0	35.0	41.0	30.0	41.0
38	36.25122279888355	39.0	35.0	41.0	30.0	41.0
39	36.161721389518796	39.0	35.0	41.0	30.0	41.0
40	36.112474274621206	39.0	35.0	41.0	29.0	41.0
41	36.13164322537304	39.0	35.0	41.0	30.0	41.0
42	35.91582515885035	39.0	35.0	40.0	29.0	41.0
43	35.960991570172304	39.0	35.0	40.0	29.0	41.0
44	35.76631993762099	38.0	35.0	40.0	28.0	41.0
45	35.59097353808611	38.0	35.0	40.0	27.0	41.0
46	35.68052474019381	38.0	35.0	40.0	28.0	41.0
47	35.56749437687206	38.0	35.0	40.0	27.0	41.0
48	35.59415357881156	38.0	35.0	40.0	28.0	41.0
49	35.51043816986146	38.0	35.0	40.0	27.0	41.0
50	35.041493882710476	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	4.0
12	6.0
13	10.0
14	22.0
15	60.0
16	150.0
17	429.0
18	1226.0
19	2495.0
20	4893.0
21	8340.0
22	13111.0
23	20242.0
24	29683.0
25	43544.0
26	59172.0
27	69130.0
28	75365.0
29	86249.0
30	105279.0
31	133705.0
32	172844.0
33	228961.0
34	344325.0
35	518397.0
36	458122.0
37	693884.0
38	841662.0
39	868500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.764814648606546	23.803692738607136	29.18089054948384	13.250602063302477
2	31.212078798898617	25.14851522434037	30.11055034998231	13.528855626778705
3	28.86872352537641	25.53953470564643	32.38781517184877	13.203926597128381
4	25.01114583352947	28.05904749830171	31.84846352775726	15.08134314041156
5	22.786100627786066	32.822832190296985	30.174360377696786	14.216706804220165
6	20.12651959396738	40.83412886654771	28.08664272012315	10.952708819361762
7	87.91293717975996	3.5280878143140746	6.861419055515351	1.6975559504106124
8	54.023912650976094	40.32308376917674	3.9933989049362393	1.6596046749109221
9	82.6557440636276	5.467452387781697	8.815763294505455	3.061040254085254
10	41.123575336524674	34.87059012559696	13.638525189165351	10.367309348713015
11	32.412962599164445	24.2740877101259	29.165597064152927	14.147352626556732
12	31.053013998664802	23.468303048675754	28.62166365085831	16.857019301801138
13	21.802045393826077	32.351558523314615	29.73919691000464	16.10719917285467
14	17.498215934389062	35.08662368172144	29.794324589685832	17.620835794203664
15	16.505896778807035	25.81824853817503	41.54137410815026	16.134480574867677
16	19.250564823351876	23.83358930569041	39.07650780480324	17.83933806615447
17	19.02576523391187	24.025270444680576	30.72523966941803	26.22372465198952
18	22.156285193583933	24.83597161646282	33.378920890838195	19.628822299115047
19	25.167992973783704	26.610538947862604	28.50073841800924	19.720729660344453
20	28.14436464355405	25.119790251208574	27.404921489606394	19.330923615630986
21	23.156512608338442	28.874100304760873	28.7177761975207	19.251610889379982
22	22.669275973767174	25.601440893189753	28.481365275168713	23.247917857874356
23	19.739056737156872	30.416671112447286	28.084780722593123	21.75949142780272
24	19.742927181460864	25.486603764624267	35.152776897338875	19.617692156576
25	18.573446283358784	27.47609582215873	32.827623172705714	21.122834721776773
26	19.091646472361994	31.16833231760322	29.59067645533413	20.149344754700653
27	19.674409856619913	30.447007027262362	29.814994854401206	20.063588261716514
28	17.74519212362492	29.491948743601476	34.340945974246274	18.42191315852733
29	18.970595711589556	26.206401798564087	33.59229743925128	21.230705050595073
30	20.03624409574182	28.191249322933764	31.19950508524078	20.573001496083634
31	24.906894893168417	27.196189474358096	27.033902790757715	20.863012841715776
32	25.332204418875804	27.894438548118934	27.716147054288527	19.05720997871674
33	23.15638708041507	29.0714929642645	27.477016360263466	20.29510359505696
34	20.49904462789653	28.61220721396423	29.44949938418093	21.43924877395831
35	20.05080533485306	28.358640808751307	30.61138584291896	20.97916801347668
36	24.445056741759565	28.423601509096695	27.75286397187505	19.378477777268692
37	20.923852041910425	30.0524518427813	28.926529134089556	20.097166981218724
38	21.29777880431724	30.775178861599816	26.9716618620854	20.955380471997547
39	21.04230855893316	29.001573910945886	28.735475634716252	21.220641895404697
40	22.911147360785872	26.46561696032878	28.782151100890346	21.841084577994994
41	18.89170141174979	26.401053765074074	29.17407019898059	25.53317462419555
42	22.912067898890605	26.917099058059385	26.92011172822033	23.250721314829683
43	21.850833913376945	27.757717718245463	28.046662076528932	22.344786291848656
44	21.473204077230637	29.556574702817873	28.344267861525125	20.625953358426365
45	19.37726434067609	31.27865044092729	27.050367870040105	22.293717348356516
46	21.375710723411146	30.32980578947335	27.44883534146629	20.84564814564921
47	21.41648637718672	27.563274964941098	28.49737008539874	22.522868572473442
48	22.197562959053	25.942039991941108	29.29009984281812	22.570297206187774
49	21.420168529605657	25.110647634122923	30.341500807667583	23.127683028603837
50	19.715331959639425	29.328511387370177	29.11415153689067	21.84200511609973
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1765.0
1	2877.5
2	3990.0
3	22880.5
4	41771.0
5	30930.0
6	20089.0
7	20525.0
8	20961.0
9	21640.5
10	22320.0
11	22804.5
12	23289.0
13	22910.0
14	22531.0
15	22019.5
16	21508.0
17	20287.5
18	19067.0
19	19303.5
20	19540.0
21	21077.5
22	22615.0
23	27218.0
24	31821.0
25	39186.0
26	46551.0
27	56259.5
28	65968.0
29	78261.0
30	90554.0
31	103701.5
32	116849.0
33	130140.0
34	143431.0
35	161214.5
36	178998.0
37	201648.0
38	224298.0
39	261817.0
40	299336.0
41	342216.5
42	385097.0
43	412967.5
44	440838.0
45	452662.0
46	464486.0
47	455967.0
48	447448.0
49	425979.0
50	404510.0
51	362541.0
52	320572.0
53	281348.0
54	242124.0
55	212022.5
56	181921.0
57	164499.0
58	147077.0
59	131993.0
60	116909.0
61	98943.0
62	80977.0
63	64594.0
64	48211.0
65	36780.5
66	25350.0
67	18975.0
68	12600.0
69	10597.5
70	8595.0
71	7663.5
72	6732.0
73	5537.5
74	4343.0
75	3483.5
76	2624.0
77	1903.0
78	1182.0
79	850.0
80	518.0
81	396.0
82	274.0
83	179.5
84	85.0
85	65.5
86	46.0
87	33.0
88	20.0
89	15.0
90	10.0
91	9.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4779813.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.900470682643906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52518767822401	20.597397919915956
2	7.328337587343029	3.7961478566699096
3	2.516520015442778	1.9553715864678676
4	1.288410624108526	1.3348176638771925
5	0.8648429695751365	1.1199919989285758
6	0.6152114437453401	0.9560559577411926
7	0.5067394569638678	0.9187353314182085
8	0.41971644122232077	0.8696682704721882
9	0.3780457608843477	0.881240683183457
>10	4.90539499268547	30.50531620309348
>50	1.2857570468349762	23.10017314818026
>100	0.3628170182764844	12.62365137970666
>500	0.0019477191573380168	0.3192276220737142
>1k	7.790876628795345E-4	0.2683772043825585
>5k	1.9477191571988362E-4	0.40872032141118214
>10k+	9.738595785994181E-5	0.3451068524776471
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13682	0.28624550793095876	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8457	0.1769316079938692	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7747	0.1620774703947623	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04349542544865249	0.0
2	0.0	0.0	0.0	0.14605173884417655	0.0
3	0.0	0.0	0.0	0.2293813586431101	0.0
4	0.0	0.0	0.0	0.3338624335303494	0.0
5	0.0	0.0	0.0	0.5579506980712425	0.0
6	0.0	0.0	0.0	0.9059768656221489	0.0
7	0.0	0.0	0.0	1.1629534460866984	0.0
8	0.0	0.0	0.0	1.9150330776538746	0.0
9	0.0	0.0	0.0	2.612068714822107	0.0
10	0.0	0.0	0.0	3.5612271860844764	0.0
11	0.0	0.0	0.0	4.089302238393008	0.0
12	0.0	0.0	0.0	4.600493785007907	0.0
13	0.0	0.0	0.0	4.845294156905301	0.0
14	0.0	0.0	0.0	4.943352386379969	0.0
15	0.0	0.0	0.0	5.058503334753891	0.0
16	0.0	0.0	0.0	5.285583348135168	0.0
17	0.0	0.0	0.0	5.550530951733886	0.0
18	0.0	0.0	0.0	5.955170212725895	0.0
19	0.0	0.0	0.0	6.132771302977752	0.0
20	0.0	0.0	0.0	6.337067998266877	0.0
21	0.0	0.0	0.0	6.546929764825528	0.0
22	0.0	0.0	0.0	6.771624747662722	0.0
23	0.0	0.0	0.0	7.016592490124614	0.0
24	0.0	0.0	0.0	7.201306829367592	0.0
25	0.0	0.0	0.0	7.360078731113539	0.0
26	0.0	0.0	0.0	7.515691513454606	0.0
27	0.0	0.0	0.0	7.676848445744635	0.0
28	0.0	0.0	0.0	7.840515936502118	0.0
29	2.0921320562122408E-5	0.0	0.0	8.017426623175426	0.0
30	2.0921320562122408E-5	0.0	0.0	8.261076322441903	0.0
31	2.0921320562122408E-5	0.0	0.0	8.46459892887023	0.0
32	2.0921320562122408E-5	0.0	0.0	8.661907903091606	0.0
33	2.0921320562122408E-5	0.0	0.0	8.84963491249553	0.0
34	4.1842641124244816E-5	0.0	0.0	9.041964612423122	0.0
35	6.276396168636723E-5	0.0	0.0	9.256157092338132	0.0
36	6.276396168636723E-5	0.0	0.0	9.457127297657879	0.0
37	6.276396168636723E-5	0.0	0.0	9.668244343450256	0.0
38	6.276396168636723E-5	0.0	0.0	9.88247866600639	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGAA	20	7.8589807E-4	44.0	15
CGTTTTT	18605	0.0	42.604675	1
AGCGATA	700	0.0	38.657143	8
CGACGGT	245	0.0	38.612244	28
GAGCGAT	3650	0.0	38.39452	7
AGACCGA	1145	0.0	38.23581	8
CGCTCGA	35	7.295035E-6	37.714287	2
GACCGAT	2800	0.0	37.63571	9
ACGAGAC	605	0.0	36.727272	5
GTTGCGA	205	0.0	36.4878	2
GAGACCG	1300	0.0	36.384613	7
CGGTCTA	230	0.0	36.347828	31
CTACGCG	85	0.0	36.23529	1
CTTGCGA	280	0.0	36.142857	2
AATCGCG	140	0.0	36.142857	1
TTAGCGA	335	0.0	36.119404	2
CGAGGTT	715	0.0	36.0	6
CCCGGTA	55	3.428795E-9	36.0	21
GTTAGCG	245	0.0	35.918365	1
CACAACG	1440	0.0	35.75	12
>>END_MODULE