##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546763_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3287594
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.432291213574427	31.0	31.0	33.0	30.0	34.0
2	31.823426493660712	31.0	31.0	34.0	30.0	34.0
3	31.92653229078773	31.0	31.0	34.0	30.0	34.0
4	35.64500634810746	37.0	35.0	37.0	33.0	37.0
5	35.52244589812489	37.0	35.0	37.0	33.0	37.0
6	35.5714939253448	37.0	35.0	37.0	33.0	37.0
7	35.99505200459667	37.0	35.0	37.0	35.0	37.0
8	22.048674197604694	35.0	0.0	37.0	0.0	37.0
9	29.920674511512065	37.0	18.0	39.0	17.0	39.0
10	34.27388661738645	35.0	32.0	39.0	27.0	39.0
11	35.87905136704836	37.0	35.0	39.0	32.0	39.0
12	36.3732459056684	37.0	35.0	39.0	32.0	39.0
13	36.507339714088786	39.0	35.0	39.0	32.0	39.0
14	37.64227638814282	39.0	36.0	41.0	33.0	41.0
15	37.901371337215	40.0	36.0	41.0	33.0	41.0
16	37.94716470464419	40.0	36.0	41.0	33.0	41.0
17	37.907774500135965	40.0	36.0	41.0	33.0	41.0
18	37.83275580865521	39.0	36.0	41.0	33.0	41.0
19	37.80962795284333	39.0	36.0	41.0	33.0	41.0
20	37.6741811793062	39.0	35.0	41.0	33.0	41.0
21	37.58418284009522	39.0	35.0	41.0	33.0	41.0
22	37.546377381148645	39.0	35.0	41.0	33.0	41.0
23	37.39451039270664	39.0	35.0	41.0	32.0	41.0
24	37.24447331391893	39.0	35.0	41.0	32.0	41.0
25	37.107199064118014	39.0	35.0	41.0	32.0	41.0
26	37.049735460035514	39.0	35.0	41.0	32.0	41.0
27	36.91998951208696	39.0	35.0	41.0	32.0	41.0
28	36.92757104435645	39.0	35.0	41.0	32.0	41.0
29	36.85780695548173	39.0	35.0	41.0	31.0	41.0
30	36.735716454039036	39.0	35.0	40.0	31.0	41.0
31	36.62894749169149	39.0	35.0	41.0	31.0	41.0
32	36.276295369805396	39.0	35.0	41.0	30.0	41.0
33	36.056281888822035	39.0	35.0	41.0	29.0	41.0
34	35.86954623959041	39.0	35.0	41.0	28.0	41.0
35	35.673804003779054	39.0	35.0	41.0	27.0	41.0
36	35.537377790566595	39.0	35.0	41.0	25.0	41.0
37	35.505487599746196	39.0	35.0	41.0	25.0	41.0
38	35.42060211814476	39.0	35.0	40.0	25.0	41.0
39	35.33340947817766	38.0	35.0	40.0	24.0	41.0
40	35.281908289162224	38.0	35.0	40.0	24.0	41.0
41	35.28261305988513	38.0	35.0	40.0	24.0	41.0
42	35.034901511561344	38.0	34.0	40.0	23.0	41.0
43	35.07593121291741	38.0	35.0	40.0	24.0	41.0
44	34.89562306051173	38.0	34.0	40.0	23.0	41.0
45	34.706437595396515	38.0	34.0	40.0	23.0	41.0
46	34.78255648355606	38.0	34.0	40.0	23.0	41.0
47	34.658076696818405	38.0	34.0	40.0	23.0	41.0
48	34.67518069445315	38.0	34.0	40.0	23.0	41.0
49	34.60735023850268	38.0	34.0	40.0	23.0	41.0
50	34.13605572950918	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	6.0
14	9.0
15	51.0
16	142.0
17	390.0
18	1178.0
19	2593.0
20	4930.0
21	8379.0
22	13030.0
23	19435.0
24	29565.0
25	44028.0
26	58654.0
27	65106.0
28	64865.0
29	67081.0
30	77947.0
31	96395.0
32	123454.0
33	160269.0
34	237856.0
35	359163.0
36	306824.0
37	450406.0
38	540946.0
39	554885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.492207979452452	23.948790513670485	29.391737544234473	16.16726396264259
2	32.59617823855378	25.408916064453212	29.228092033262016	12.766813663730984
3	25.79917106552695	24.880444483108317	36.29447553438776	13.025908916976976
4	23.695565815000272	27.14282846361199	35.13058485932265	14.031020862065086
5	21.14646759910135	31.306420439993506	34.24297525789377	13.304136703011382
6	19.32574399393599	39.04448055325566	31.111992539224737	10.517782913583613
7	83.94804832956868	3.4879915220675057	10.602282398617348	1.961677749746471
8	52.14898798330937	39.736719315097915	6.1037950549855005	2.010497646607215
9	78.96863177144137	5.236291342544122	12.636688106864776	3.1583887791497367
10	39.953412738920925	32.197710544550205	17.40360883977766	10.445267876751204
11	31.18694096655487	23.797981137573558	31.11159711326885	13.903480782602717
12	29.64091673120221	22.517105214329995	31.279683561899674	16.56229449256812
13	20.66371334173259	32.064573666943055	32.59706034260921	14.674652648715139
14	16.327989405017775	34.97223197268276	31.8052350746473	16.894543547652173
15	14.985396615275487	25.10337347008177	44.29199590947057	15.619234005172173
16	16.40108237209339	23.234559985204985	42.78025206275471	17.584105579946915
17	17.514480194330563	23.46415037866598	32.81241540165848	26.208954025344976
18	20.18956720324955	24.488455691304946	36.39248033668391	18.92949676876159
19	23.73936684395944	26.82667020319419	30.435783737286293	18.99817921556007
20	25.05358021702193	25.17302927307934	30.118408781619628	19.6549817282791
21	20.920010195906187	28.72979449408899	31.659474983833164	18.69072032617166
22	20.97053346611534	24.91992624393401	30.557939940272433	23.551600349678214
23	17.50867047451723	29.880514443085126	30.492116727308787	22.11869835508886
24	18.178278704730573	25.382513777552823	37.79298782027221	18.64621969744439
25	17.18147070471597	27.135893300693454	35.53455809932735	20.14807789526322
26	17.201728680609587	30.912789109604166	31.96948893324419	19.915993276542054
27	18.116713925137958	29.660870533283614	32.45589327635955	19.76652226521888
28	16.401903641386376	27.819858534843416	37.48966569472994	18.288572129040265
29	16.773269448721468	24.446418870456633	36.72990034657564	22.050411334246263
30	18.39022701708301	27.721823315166045	33.19786445649919	20.690085211251755
31	22.85768863186878	25.687478441681062	29.818067559437083	21.63676536701308
32	23.286208698519342	26.265560771798462	29.92458922847529	20.5236413012069
33	21.62815724812735	27.253669400783675	28.92403989057043	22.194133460518543
34	17.799795230189616	27.391247216049187	31.776399397249172	23.032558156512025
35	18.119056063492025	26.631694789563433	31.31022261264621	23.939026534298335
36	23.346283026432097	25.890940304672654	30.67799734395427	20.084779324940975
37	19.098860747403723	30.555384880249814	30.63623427953695	19.709520092809512
38	20.051928553221597	30.470489969260196	28.72146013163426	20.75612134588395
39	19.56211746340941	31.232567038387344	28.98350587085875	20.221809627344495
40	22.062639121497362	26.999410511151922	29.071260015683205	21.86669035166751
41	18.057947544617736	26.630903937651667	29.27672942583543	26.034419091895167
42	21.17031482597912	27.489130348820446	27.73979998746804	23.600754837732396
43	20.525740100511193	27.510787524250258	29.389182484211858	22.574289891026687
44	20.349623463237858	29.641950922163744	29.481012558120014	20.527413056478387
45	18.108166641014677	31.530687791740707	27.90803852300497	22.453107044239648
46	21.37274249800918	29.810371961988007	28.74825784449053	20.06862769551228
47	20.473300535285073	27.24858969811966	29.224198608465645	23.053911158129623
48	21.243529462579623	25.070766037412163	30.731410265379484	22.954294234628726
49	20.51235645277367	24.237786052657352	31.51772998734029	23.73212750722869
50	19.05384302319569	28.329714678880663	30.53351478315145	22.0829275147722
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1618.0
1	2242.0
2	2866.0
3	24137.5
4	45409.0
5	34002.5
6	22596.0
7	23383.5
8	24171.0
9	25399.0
10	26627.0
11	26917.0
12	27207.0
13	26579.5
14	25952.0
15	25112.5
16	24273.0
17	23296.5
18	22320.0
19	21719.5
20	21119.0
21	21810.0
22	22501.0
23	24054.0
24	25607.0
25	28393.0
26	31179.0
27	36894.5
28	42610.0
29	51619.5
30	60629.0
31	70992.5
32	81356.0
33	90828.5
34	100301.0
35	110943.5
36	121586.0
37	136008.0
38	150430.0
39	176853.0
40	203276.0
41	232550.0
42	261824.0
43	283478.0
44	305132.0
45	318993.5
46	332855.0
47	329710.0
48	326565.0
49	302725.0
50	278885.0
51	242078.5
52	205272.0
53	178564.5
54	151857.0
55	131322.5
56	110788.0
57	95431.0
58	80074.0
59	69213.5
60	58353.0
61	48712.0
62	39071.0
63	31130.5
64	23190.0
65	17785.0
66	12380.0
67	9595.5
68	6811.0
69	5685.5
70	4560.0
71	3653.5
72	2747.0
73	2224.5
74	1702.0
75	1377.0
76	1052.0
77	800.5
78	549.0
79	367.5
80	186.0
81	121.5
82	57.0
83	46.0
84	35.0
85	21.5
86	8.0
87	5.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3287594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.750597126407207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8183276942602	23.151417162826725
2	7.290080876312399	4.337685183401916
3	2.8558152870189755	2.548866302146095
4	1.6480437387692535	1.961211412752878
5	1.0966236213040719	1.6312603778359598
6	0.8396842964738941	1.4988665530659298
7	0.6476873469635293	1.3488359726368437
8	0.564411284282155	1.3433258185821189
9	0.4851859075368562	1.2991113419885343
>10	5.876406485789549	39.44708920264189
>50	0.7763143323934267	15.499701341621552
>100	0.09836336032107795	4.1398980663601135
>500	0.0018552880631292092	0.4063069644017251
>1k	9.822113275389332E-4	0.5330627890000966
>5k	0.0	0.0
>10k+	2.182691838975407E-4	0.8533615107375772
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16269	0.49486037509497827	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10014	0.30459965555357504	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	4884	0.14855848988652492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08230943358577732	0.0
2	0.0	0.0	0.0	0.2542588896317489	0.0
3	0.0	0.0	0.0	0.3944830170635425	0.0
4	0.0	0.0	0.0	0.55989273614686	0.0
5	0.0	0.0	0.0	0.9120955933123129	0.0
6	0.0	0.0	0.0	1.4859803248211305	0.0
7	0.0	0.0	0.0	1.8933907289038732	0.0
8	0.0	0.0	0.0	2.963109191706762	0.0
9	0.0	0.0	0.0	3.8836608169986926	0.0
10	0.0	0.0	0.0	5.042715128449559	0.0
11	0.0	0.0	0.0	5.703137309533963	0.0
12	0.0	0.0	0.0	6.3679700108955055	0.0
13	0.0	0.0	0.0	6.708431758909403	0.0
14	0.0	3.04173812216472E-5	0.0	6.85166720708214	0.0
15	0.0	3.04173812216472E-5	0.0	6.999161088625907	0.0
16	0.0	3.04173812216472E-5	0.0	7.275016318925025	0.0
17	0.0	3.04173812216472E-5	0.0	7.5943684043710995	0.0
18	0.0	3.04173812216472E-5	0.0	8.100239871468315	0.0
19	0.0	3.04173812216472E-5	0.0	8.308142672118272	0.0
20	0.0	3.04173812216472E-5	0.0	8.56331408318667	0.0
21	0.0	3.04173812216472E-5	0.0	8.821527232377234	0.0
22	3.04173812216472E-5	3.04173812216472E-5	0.0	9.09513157646595	0.0
23	3.04173812216472E-5	3.04173812216472E-5	0.0	9.42290927651042	0.0
24	3.04173812216472E-5	3.04173812216472E-5	0.0	9.660681945520038	0.0
25	3.04173812216472E-5	3.04173812216472E-5	0.0	9.868250154976558	0.0
26	3.04173812216472E-5	3.04173812216472E-5	0.0	10.067666506265676	0.0
27	3.04173812216472E-5	3.04173812216472E-5	0.0	10.278398123369248	0.0
28	3.04173812216472E-5	3.04173812216472E-5	0.0	10.492597321932088	0.0
29	3.04173812216472E-5	3.04173812216472E-5	0.0	10.721305611337653	0.0
30	3.04173812216472E-5	3.04173812216472E-5	0.0	11.007320246964802	0.0
31	3.04173812216472E-5	3.04173812216472E-5	0.0	11.25360978271648	0.0
32	3.04173812216472E-5	3.04173812216472E-5	0.0	11.48207473307227	0.0
33	3.04173812216472E-5	3.04173812216472E-5	0.0	11.707315441018569	0.0
34	6.08347624432944E-5	3.04173812216472E-5	0.0	11.937635851628881	0.0
35	6.08347624432944E-5	3.04173812216472E-5	0.0	12.192716010553614	0.0
36	6.08347624432944E-5	3.04173812216472E-5	0.0	12.429424071220472	0.0
37	6.08347624432944E-5	3.04173812216472E-5	0.0	12.67331063385564	0.0
38	6.08347624432944E-5	3.04173812216472E-5	0.0	12.917379700778138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	22980	0.0	43.100086	1
TTCGCGA	100	0.0	41.8	2
GACGGTC	470	0.0	41.659576	29
GAGCGAT	2160	0.0	40.84259	7
CGACGGT	465	0.0	40.68817	28
AGCGATA	560	0.0	40.07143	8
TTTAGCG	275	0.0	40.0	1
CGGTCTA	480	0.0	39.875	31
CACGACG	475	0.0	39.36842	26
TATGCGA	205	0.0	38.634148	2
CGCTCGA	40	4.1276508E-7	38.500004	2
GTTGCGA	115	0.0	38.260868	2
TTTGCGA	260	0.0	38.076923	2
GTTTTTA	10910	0.0	37.97067	2
CGTAAGA	215	0.0	37.860466	2
AGCGATC	280	0.0	37.714287	8
TCCGTCG	35	7.294342E-6	37.714287	15
TACTCGC	35	7.294342E-6	37.714287	12
TTAGCGA	275	0.0	37.6	2
TCACGAC	510	0.0	37.52941	25
>>END_MODULE