FastQCFastQC Report
Thu 26 May 2016
SRR1546762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546762_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2143080
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64270.2998954775370028No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40730.19005356776228605No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA34670.16177650857644138No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC254.4441338E-544.08
ACGTTAC207.8580074E-444.027
TACATCG254.4441338E-544.032
CGCTAAA207.8580074E-444.010
TCGAATC302.5287463E-644.012
CGACGGT2250.043.02222428
CGTTTTT88300.042.8040771
TACGTAG1100.042.01
GAGCGAT13650.039.9706957
TAATGCG1050.039.8095251
CGGTCTA2550.039.68627531
CGTTCGA501.3496901E-939.614
TTCGCGA501.3496901E-939.62
TCACGAC2600.038.92307725
CACGACG2450.038.61224426
GCGCGAC4200.038.2380949
TCTAGCG1400.037.7142871
CGAGGTT2450.037.7142876
AGCGATA2800.037.7142878
CCCGACG1050.037.71428710
Produced by FastQC (version 0.11.5)