##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546762_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2143080
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41608992664763	31.0	31.0	33.0	30.0	34.0
2	31.81021333781287	31.0	31.0	34.0	30.0	34.0
3	31.94778449707897	31.0	31.0	34.0	30.0	34.0
4	35.651635029956886	37.0	35.0	37.0	33.0	37.0
5	35.52333510648226	37.0	35.0	37.0	33.0	37.0
6	35.57647218022659	37.0	35.0	37.0	33.0	37.0
7	35.98133060828341	37.0	35.0	37.0	35.0	37.0
8	22.111697650111054	35.0	0.0	37.0	0.0	37.0
9	29.949076096086007	37.0	18.0	39.0	17.0	39.0
10	34.24823525020064	35.0	32.0	39.0	27.0	39.0
11	35.85502594396849	37.0	35.0	39.0	32.0	39.0
12	36.315359202642924	37.0	35.0	39.0	32.0	39.0
13	36.44537441439424	39.0	35.0	39.0	32.0	39.0
14	37.55553642421188	39.0	36.0	41.0	33.0	41.0
15	37.80746449035967	40.0	36.0	41.0	33.0	41.0
16	37.865743229370814	40.0	36.0	41.0	33.0	41.0
17	37.82591363831495	40.0	36.0	41.0	33.0	41.0
18	37.78486337420908	39.0	36.0	41.0	33.0	41.0
19	37.79673553950389	40.0	36.0	41.0	33.0	41.0
20	37.685794744013286	39.0	36.0	41.0	33.0	41.0
21	37.58972273550217	39.0	35.0	41.0	33.0	41.0
22	37.54644670287623	39.0	35.0	41.0	33.0	41.0
23	37.42541482352502	39.0	35.0	41.0	32.0	41.0
24	37.32631073035071	39.0	35.0	41.0	32.0	41.0
25	37.184436418612464	39.0	35.0	41.0	32.0	41.0
26	37.1299615506654	39.0	35.0	41.0	32.0	41.0
27	37.020250293969426	39.0	35.0	41.0	32.0	41.0
28	37.035552102581335	39.0	35.0	41.0	32.0	41.0
29	36.96395328219199	39.0	35.0	41.0	31.0	41.0
30	36.86189782929242	39.0	35.0	41.0	31.0	41.0
31	36.801006495324486	39.0	35.0	41.0	31.0	41.0
32	36.55376700823114	39.0	35.0	41.0	30.0	41.0
33	36.440122627246765	39.0	35.0	41.0	30.0	41.0
34	36.354619052951826	39.0	35.0	41.0	30.0	41.0
35	36.207087929522	39.0	35.0	41.0	30.0	41.0
36	36.07803348451761	39.0	35.0	41.0	30.0	41.0
37	36.063550124120425	39.0	35.0	41.0	29.0	41.0
38	35.97716324168953	39.0	35.0	41.0	29.0	41.0
39	35.90240821621218	39.0	35.0	40.0	29.0	41.0
40	35.83996117737089	39.0	35.0	40.0	29.0	41.0
41	35.85001633163484	39.0	35.0	40.0	29.0	41.0
42	35.61283386527801	38.0	35.0	40.0	27.0	41.0
43	35.649118558336596	38.0	35.0	40.0	28.0	41.0
44	35.45977518338093	38.0	35.0	40.0	27.0	41.0
45	35.23770741176251	38.0	34.0	40.0	26.0	41.0
46	35.32011824103627	38.0	34.0	40.0	26.0	41.0
47	35.19440478190268	38.0	34.0	40.0	26.0	41.0
48	35.222299214215056	38.0	34.0	40.0	26.0	41.0
49	35.15445433674898	38.0	34.0	40.0	26.0	41.0
50	34.68489463762435	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	5.0
13	6.0
14	8.0
15	25.0
16	79.0
17	250.0
18	628.0
19	1379.0
20	2705.0
21	4457.0
22	6904.0
23	10295.0
24	14936.0
25	21925.0
26	28505.0
27	33222.0
28	36549.0
29	41072.0
30	49878.0
31	63312.0
32	81795.0
33	107678.0
34	162078.0
35	244525.0
36	204841.0
37	303180.0
38	360190.0
39	362649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.054603654553258	24.853015286410212	30.556815424529184	13.535565634507343
2	30.59428486104112	25.81854153834668	30.279830897586653	13.307342703025551
3	27.241633536778842	25.599837616887843	33.40570580659612	13.7528230397372
4	25.109281968008663	27.756313343412288	32.57442559307165	14.5599790955074
5	22.362860929130036	32.17406723034138	31.194495772439666	14.26857606808892
6	19.832624073762993	40.801136681785096	28.081826156746363	11.284413087705545
7	86.97276816544412	3.5331858820016047	7.501399854415141	1.992646098139127
8	54.06069768744051	39.7094368852306	4.2724956604513125	1.9573697668775782
9	81.87067211676651	5.266438957015137	9.474587976183809	3.38830095003453
10	42.358708027698455	30.2124045765907	15.571000615935942	11.857886779774903
11	33.51461448009407	24.22294081415533	27.247512925322432	15.01493178042817
12	31.373397166694666	22.78976986393415	28.72155962446572	17.115273344905464
13	21.716174851148814	32.84357093528939	29.63057841984434	15.809675793717453
14	17.345642719823807	36.312736808705225	28.90568714187058	17.435933329600388
15	15.452806241484218	25.705993243369356	42.70554528995651	16.13565522518991
16	17.310926330328314	22.989995707113124	40.36746178397447	19.331616178584092
17	17.71851727420348	22.899705097336543	30.755594751479183	28.626182876980792
18	21.368357690800156	24.12518431416466	34.103719879799165	20.402738115236016
19	24.6239057804655	26.872398603878526	28.219105213057844	20.28459040259813
20	26.933525580006346	25.158370196166263	27.53975586539	20.368348358437387
21	22.29436138641581	28.417837878194003	29.137689680273255	20.150111055116934
22	21.838848759728986	25.1328928458107	28.260167609235303	24.768090785225002
23	19.022015043768782	29.62441906041772	27.665136159172782	23.688429736640725
24	19.390643373089198	25.82260111615059	35.176568303563094	19.610187207197118
25	18.37224928607425	27.16524814752599	32.8757209250238	21.586781641375964
26	17.806988073240383	31.48491890176755	29.360873135860537	21.34721988913153
27	18.849273008940404	30.3449241278907	29.828657819586763	20.977145043582134
28	17.038887955652612	28.341592474382665	35.582712731209284	19.036806838755435
29	17.587210930063275	25.162803068480876	34.72254885491909	22.527437146536762
30	19.36320622655244	28.069694085148477	31.371530694141143	21.19556899415794
31	24.275995296489164	26.79792634899304	26.789433898874513	22.136644455643278
32	24.195317020363216	27.283489183791552	27.889019541967635	20.632174253877597
33	22.94422046773802	27.224461989286446	27.645631520988484	22.185686021987046
34	19.071149933740227	28.570328685816676	29.169699684566137	23.188821695876964
35	19.227980290049835	26.731059969763145	30.797917016630272	23.24304272355675
36	24.775743322694442	26.068415551449313	28.41555144931594	20.740289676540307
37	20.299288873957106	30.270498534819048	29.5056180823861	19.92459450883775
38	20.984097653844	30.16462287922056	27.094415514119863	21.756863952815575
39	20.64645276891203	28.9936913227691	28.11084980495362	22.24900610336525
40	22.08620303488437	26.080081004908823	28.31098232450492	23.522733635701886
41	18.371362711611326	25.86944957724397	28.076600033596506	27.682587677548202
42	21.87636485805476	26.903428710080817	26.39322843757583	24.826977994288594
43	21.578849133023496	27.012804001717157	27.709931500457287	23.69841536480206
44	20.48350971498964	29.210295462605224	28.534212441906043	21.771982380499093
45	18.813530059540472	31.23873117195812	27.24336002389085	22.70437874461056
46	21.7292868208373	29.4502771711742	28.155645146238122	20.66479086175038
47	21.33023498889449	27.021109804580323	28.468652593463613	23.180002613061575
48	21.43900367695093	25.396065475857178	29.500158650167048	23.664772197024842
49	21.073781660040687	24.135916531347405	30.660591298504958	24.12971051010695
50	19.632818186908562	28.386761110177876	29.337682214383037	22.642738488530526
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1187.0
1	1665.5
2	2144.0
3	10299.0
4	18454.0
5	13805.5
6	9157.0
7	9580.5
8	10004.0
9	10604.0
10	11204.0
11	11331.0
12	11458.0
13	11352.0
14	11246.0
15	10832.5
16	10419.0
17	10265.0
18	10111.0
19	10051.0
20	9991.0
21	10619.0
22	11247.0
23	12845.5
24	14444.0
25	16710.5
26	18977.0
27	22386.0
28	25795.0
29	30033.5
30	34272.0
31	39427.5
32	44583.0
33	51566.5
34	58550.0
35	68179.5
36	77809.0
37	84836.0
38	91863.0
39	107737.5
40	123612.0
41	147185.5
42	170759.0
43	185170.5
44	199582.0
45	208069.5
46	216557.0
47	215219.5
48	213882.0
49	201179.0
50	188476.0
51	166001.0
52	143526.0
53	124834.5
54	106143.0
55	93877.0
56	81611.0
57	72871.5
58	64132.0
59	57753.5
60	51375.0
61	44663.5
62	37952.0
63	31160.5
64	24369.0
65	19813.5
66	15258.0
67	12238.5
68	9219.0
69	7548.0
70	5877.0
71	4885.0
72	3893.0
73	2948.5
74	2004.0
75	1446.0
76	888.0
77	748.0
78	608.0
79	424.5
80	241.0
81	172.0
82	103.0
83	85.0
84	67.0
85	44.5
86	22.0
87	14.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2143080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.596185446716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0028560761116	22.789908083553804
2	6.9079517637338865	4.08898042912874
3	2.702796589083847	2.3997740722583094
4	1.5718971935473471	1.8608864337359885
5	1.149373420226801	1.7008534446279322
6	0.9038408966734492	1.6050145675364087
7	0.7582307241764578	1.570851598688695
8	0.6751190683140346	1.5984759315549033
9	0.562379614068884	1.497986221449174
>10	7.123366429172619	46.37739954603334
>50	0.5930929513814308	11.232004749868766
>100	0.04679658130754795	2.152558434572907
>500	0.001313538401181387	0.259787039748433
>1k	8.20961500738367E-4	0.5532015825843922
>5k	1.641923001476734E-4	0.3123178646582826
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6427	0.2998954775370028	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4073	0.19005356776228605	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3467	0.16177650857644138	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08595106108964667	0.0
2	0.0	0.0	0.0	0.26172611381749633	0.0
3	0.0	0.0	0.0	0.39765197752767045	0.0
4	0.0	0.0	0.0	0.5661944491106258	0.0
5	0.0	0.0	0.0	0.9171846128002688	0.0
6	0.0	0.0	0.0	1.3894954924687832	0.0
7	0.0	0.0	0.0	1.7055359575937437	0.0
8	0.0	0.0	0.0	2.5547343076320064	0.0
9	0.0	0.0	0.0	3.2590943875170315	0.0
10	0.0	0.0	0.0	4.252991022267017	0.0
11	0.0	0.0	0.0	4.89757731862553	0.0
12	0.0	0.0	0.0	5.500027997088303	0.0
13	0.0	0.0	0.0	5.789097933814883	0.0
14	0.0	0.0	0.0	5.906405733803685	0.0
15	0.0	0.0	0.0	6.047791029732908	0.0
16	0.0	0.0	0.0	6.335601097485862	0.0
17	0.0	0.0	0.0	6.67581238217892	0.0
18	0.0	0.0	0.0	7.204210762080743	0.0
19	0.0	0.0	0.0	7.417781884017396	0.0
20	0.0	0.0	0.0	7.671388842227075	0.0
21	0.0	0.0	0.0	7.928682083729958	0.0
22	0.0	0.0	0.0	8.20403344718816	0.0
23	0.0	0.0	0.0	8.52362021016481	0.0
24	0.0	0.0	0.0	8.76108218078653	0.0
25	0.0	0.0	0.0	8.960701420385613	0.0
26	0.0	0.0	0.0	9.148795191966702	0.0
27	0.0	0.0	0.0	9.356393601732087	0.0
28	4.666181383802751E-5	0.0	0.0	9.55549956137895	0.0
29	9.332362767605503E-5	0.0	0.0	9.774950071859193	0.0
30	9.332362767605503E-5	0.0	0.0	10.046708475651865	0.0
31	9.332362767605503E-5	0.0	0.0	10.283797151762883	0.0
32	9.332362767605503E-5	0.0	0.0	10.507353901860872	0.0
33	9.332362767605503E-5	0.0	0.0	10.741316236444742	0.0
34	1.3998544151408253E-4	0.0	0.0	10.970845698714001	0.0
35	1.3998544151408253E-4	0.0	0.0	11.221093006327342	0.0
36	1.3998544151408253E-4	0.0	0.0	11.44824271609086	0.0
37	1.3998544151408253E-4	0.0	0.0	11.690324206282547	0.0
38	1.3998544151408253E-4	0.0	0.0	11.93058588573455	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	25	4.4441338E-5	44.0	8
ACGTTAC	20	7.8580074E-4	44.0	27
TACATCG	25	4.4441338E-5	44.0	32
CGCTAAA	20	7.8580074E-4	44.0	10
TCGAATC	30	2.5287463E-6	44.0	12
CGACGGT	225	0.0	43.022224	28
CGTTTTT	8830	0.0	42.804077	1
TACGTAG	110	0.0	42.0	1
GAGCGAT	1365	0.0	39.970695	7
TAATGCG	105	0.0	39.809525	1
CGGTCTA	255	0.0	39.686275	31
CGTTCGA	50	1.3496901E-9	39.6	14
TTCGCGA	50	1.3496901E-9	39.6	2
TCACGAC	260	0.0	38.923077	25
CACGACG	245	0.0	38.612244	26
GCGCGAC	420	0.0	38.238094	9
TCTAGCG	140	0.0	37.714287	1
CGAGGTT	245	0.0	37.714287	6
AGCGATA	280	0.0	37.714287	8
CCCGACG	105	0.0	37.714287	10
>>END_MODULE