FastQCFastQC Report
Thu 26 May 2016
SRR1546761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546761_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1917461
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA56810.2962772124178797No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42230.2202391600142063No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25600.13350988625061996No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC254.443957E-544.022
CGTACTC207.8578E-444.016
ATCATCG207.8578E-444.036
TCACGAC2650.043.16981525
CGGTCTA2600.043.15384331
CGACGGT2700.042.3703728
CGTTTTT54000.041.5555531
CACGACG2850.040.1403526
TACTACG557.8216544E-1140.01
ACGAGTA557.8216544E-1140.05
TACGTTG557.8216544E-1140.01
TCTAGCG1050.039.8095251
TATAGCG1000.039.61
ATGGTCC7900.039.2658211
GAGCGAT14000.039.128577
TACGTAG1250.038.721
TGTTGCG1650.038.6666681
ACGAGAT2050.038.6341485
GCGCGAC8900.038.56189
AGGGGAA147450.038.5242468
Produced by FastQC (version 0.11.5)