##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546761_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1917461
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.491203210912765	31.0	31.0	33.0	30.0	34.0
2	31.884035190285488	31.0	31.0	34.0	30.0	34.0
3	32.02563650577508	33.0	31.0	34.0	30.0	34.0
4	35.716268544705734	37.0	35.0	37.0	35.0	37.0
5	35.59552710589681	37.0	35.0	37.0	33.0	37.0
6	35.64262689045566	37.0	35.0	37.0	33.0	37.0
7	35.97359841999394	37.0	35.0	37.0	35.0	37.0
8	22.163570471576737	35.0	0.0	37.0	0.0	37.0
9	29.97141949692849	37.0	18.0	39.0	17.0	39.0
10	34.382515211521905	35.0	32.0	39.0	27.0	39.0
11	36.003427970634085	37.0	35.0	39.0	32.0	39.0
12	36.25095164908178	37.0	35.0	39.0	32.0	39.0
13	36.25829938653251	38.0	35.0	39.0	32.0	39.0
14	37.349595637147246	39.0	35.0	41.0	33.0	41.0
15	37.66493086430441	39.0	35.0	41.0	33.0	41.0
16	37.74642874092354	40.0	35.0	41.0	33.0	41.0
17	37.71463722078311	40.0	35.0	41.0	33.0	41.0
18	37.68336983125081	39.0	36.0	41.0	33.0	41.0
19	37.67000267541295	39.0	36.0	41.0	33.0	41.0
20	37.55620792287301	39.0	35.0	41.0	33.0	41.0
21	37.45023132152362	39.0	35.0	41.0	33.0	41.0
22	37.39960708457695	39.0	35.0	41.0	33.0	41.0
23	37.301144586513104	39.0	35.0	41.0	32.0	41.0
24	37.20970074489129	39.0	35.0	41.0	32.0	41.0
25	37.05140965057438	39.0	35.0	41.0	32.0	41.0
26	37.01740322228196	39.0	35.0	41.0	32.0	41.0
27	36.906938915576376	39.0	35.0	41.0	32.0	41.0
28	36.93292536327988	39.0	35.0	41.0	32.0	41.0
29	36.85301448112895	38.0	35.0	41.0	32.0	41.0
30	36.81584553740598	38.0	35.0	40.0	32.0	41.0
31	36.72176748314568	38.0	35.0	40.0	31.0	41.0
32	36.50878010035145	38.0	35.0	40.0	31.0	41.0
33	36.40571881253387	38.0	35.0	41.0	31.0	41.0
34	36.32715867493524	38.0	35.0	41.0	30.0	41.0
35	36.18614876651989	38.0	35.0	41.0	30.0	41.0
36	36.05140287077547	38.0	35.0	40.0	30.0	41.0
37	35.97391707054276	38.0	35.0	40.0	30.0	41.0
38	35.88849838406101	38.0	35.0	40.0	30.0	41.0
39	35.81472061230971	38.0	35.0	40.0	29.0	41.0
40	35.668355184277544	38.0	35.0	40.0	29.0	41.0
41	35.68043052766132	38.0	35.0	40.0	29.0	41.0
42	35.44731861560678	38.0	35.0	40.0	28.0	41.0
43	35.477567992256425	38.0	35.0	40.0	29.0	41.0
44	35.27671957865115	37.0	34.0	40.0	28.0	41.0
45	35.0966648083064	37.0	34.0	40.0	27.0	41.0
46	35.18544105981817	37.0	34.0	40.0	27.0	41.0
47	35.069987342636956	37.0	34.0	40.0	27.0	41.0
48	35.096647598047625	37.0	34.0	40.0	27.0	41.0
49	35.019353718276406	37.0	34.0	40.0	27.0	41.0
50	34.52107813405331	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	4.0
12	4.0
13	7.0
14	16.0
15	29.0
16	79.0
17	221.0
18	501.0
19	1069.0
20	2147.0
21	3822.0
22	6005.0
23	8787.0
24	12519.0
25	17439.0
26	22801.0
27	27210.0
28	30642.0
29	35890.0
30	44561.0
31	57089.0
32	74844.0
33	100301.0
34	161682.0
35	266400.0
36	184015.0
37	266709.0
38	313707.0
39	278959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.742809892873964	24.692131939058996	30.4494850221204	13.115573145946646
2	30.125045568071528	26.509587417944875	29.89453240509194	13.470834608891655
3	28.699410313951628	25.586283110842935	31.75021551937693	13.964091055828515
4	26.248147941470517	28.026489195868916	30.574650540480352	15.150712322180215
5	23.336693679819305	32.60014154134034	29.5811492384982	14.48201554034215
6	20.864570387611533	40.64651119370876	27.447337911957533	11.04158050672217
7	86.23752973332964	4.835404735741692	6.71033204847452	2.216733482454141
8	53.764066127029444	40.20238221272819	3.8482660142761707	2.1852856459662022
9	81.43983110999389	5.784420126406744	8.990952097591554	3.7847966660078094
10	49.61707174226751	24.59731905890133	13.249135184496582	12.536474014334582
11	44.35636500559855	20.90368461209902	21.21148748266588	13.528462899636551
12	39.20434366070549	21.052996645042583	23.956367300299718	15.78629239395221
13	22.839004287440527	37.255255778344385	25.036962942140672	14.868776992074414
14	16.272821194277224	40.427836602674056	26.947614579905405	16.351727623143315
15	14.209571928711979	22.866279939983134	46.8134162833038	16.11073184800108
16	16.01550174944888	19.02427220162496	45.16790693526492	19.792319113661243
17	16.96670753668523	19.189751447356688	28.629474080568002	35.21406693539008
18	22.045976424031572	22.65099524840401	32.847343440101255	22.45568488746316
19	28.559016324191212	25.12885529353661	25.154409920201765	21.157718462070417
20	30.685682785725497	23.019086176980913	24.226516210759957	22.06871482653363
21	23.489760678313665	27.72244129085285	26.676735537254736	22.11106249357875
22	22.37041587808044	25.582788906788718	24.65333062836741	27.393464586763432
23	20.295797411264164	30.01808120217308	23.566476710608455	26.119644675954294
24	21.01163987168448	24.23428690335814	35.10110505507022	19.65296816988716
25	18.584315404589717	24.790908393964727	33.84100119898136	22.783775002464196
26	17.60239191305586	32.89339392039786	27.402174020749314	22.102040145796966
27	19.554713237974592	30.808814364412108	28.072331066968246	21.564141330645057
28	17.69574452883266	27.463661581643645	36.82938010212463	18.011213787399065
29	17.351017830349612	24.243361403439234	35.466640520980604	22.938980245230542
30	20.68229810149985	27.442644205019036	30.364476774234262	21.51058091924686
31	27.81668049571804	25.477441262169087	24.4822710866088	22.223607155504073
32	28.952870488630538	26.026552821674077	26.103946833859986	18.9166298558354
33	26.97494238474733	26.326584999642755	26.18593024838576	20.512542367224157
34	20.997297989372406	26.870324872318136	28.095382383266205	24.03699475504326
35	20.960687075252117	24.628610438491314	30.946339977710107	23.464362508546458
36	30.109973553569013	23.026126737388662	26.714024431266136	20.149875277776182
37	22.794361919225476	30.011197098663285	27.988522321966393	19.205918660144846
38	22.996608535975437	29.88707462628966	23.778893025725164	23.337423812009735
39	23.08933532416044	29.078922596078876	26.064258934080016	21.767483145680668
40	25.334804723538053	24.852708868654954	25.806887336952357	24.005599070854636
41	19.641964034731345	23.76376885892334	26.85295815664569	29.74130894969963
42	24.02374807101683	25.288858547840086	24.386519465063436	26.30087391607965
43	24.1480270002884	25.576739240067987	26.14488638882355	24.130347370820058
44	22.02683653018236	28.822854806434133	27.398992730491003	21.751315932892506
45	18.89561247921079	33.000358286296304	24.66433476352322	23.439694470969684
46	23.2508509951441	29.93192560370198	26.38426544268697	20.43295795846695
47	23.194161445786904	26.291486502202655	27.062975465993834	23.45137658601661
48	24.172747190164493	22.68113927740903	29.113551722825132	24.032561809601344
49	22.751597033785824	22.15121976405257	30.597232486084465	24.499950716077144
50	20.336945575425002	28.876988893124818	27.731672247831902	23.054393283618285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1556.0
1	1978.5
2	2401.0
3	7251.5
4	12102.0
5	9229.0
6	6356.0
7	6916.0
8	7476.0
9	8078.0
10	8680.0
11	8907.5
12	9135.0
13	8909.0
14	8683.0
15	8224.5
16	7766.0
17	7555.0
18	7344.0
19	7252.0
20	7160.0
21	7502.0
22	7844.0
23	7992.0
24	8140.0
25	9555.5
26	10971.0
27	13085.0
28	15199.0
29	17540.5
30	19882.0
31	22976.0
32	26070.0
33	29129.5
34	32189.0
35	37188.5
36	42188.0
37	52078.5
38	61969.0
39	84234.5
40	106500.0
41	132777.0
42	159054.0
43	170007.0
44	180960.0
45	184035.0
46	187110.0
47	183551.0
48	179992.0
49	172850.5
50	165709.0
51	149645.0
52	133581.0
53	118158.0
54	102735.0
55	94733.0
56	86731.0
57	83445.0
58	80159.0
59	74369.0
60	68579.0
61	61422.5
62	54266.0
63	46872.5
64	39479.0
65	32328.5
66	25178.0
67	20460.0
68	15742.0
69	13253.5
70	10765.0
71	9371.5
72	7978.0
73	6402.5
74	4827.0
75	3608.0
76	2389.0
77	1881.5
78	1374.0
79	1068.5
80	763.0
81	525.5
82	288.0
83	195.5
84	103.0
85	81.0
86	59.0
87	39.5
88	20.0
89	11.5
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1917461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.207670424961876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2790185631844	18.707450121420788
2	6.8021961992229585	3.293306475134354
3	2.4014612735861878	1.7440134914785095
4	1.425093896603555	1.3799281349441421
5	1.065529403721056	1.2896992316692737
6	0.7994133468525128	1.1611160900352817
7	0.6555818486845443	1.1109076530679873
8	0.5123272871008242	0.992180009268126
9	0.45416519742068123	0.9894853275882838
>10	7.1435667752977965	42.10339379760627
>50	1.3121065559192173	21.05113313279416
>100	0.14670581270319583	5.132440211004044
>500	0.0017439013557383003	0.2710253671349518
>1k	8.719506778691502E-4	0.47413608327639045
>5k	2.1798766946728754E-4	0.2997848735774262
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	5681	0.2962772124178797	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4223	0.2202391600142063	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2560	0.13350988625061996	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04521604350753418	0.0
2	0.0	0.0	0.0	0.19275489827433256	0.0
3	0.0	0.0	0.0	0.31432190798144005	0.0
4	0.0	0.0	0.0	0.47025728293821883	0.0
5	0.0	0.0	0.0	0.7513581762549538	0.0
6	0.0	0.0	0.0	1.1767123294815383	0.0
7	0.0	0.0	0.0	1.4447229956697947	0.0
8	0.0	0.0	0.0	2.2136043444951423	0.0
9	0.0	0.0	0.0	2.879641359068059	0.0
10	0.0	0.0	0.0	3.8680317357171803	0.0
11	0.0	0.0	0.0	4.5077318391351895	0.0
12	0.0	0.0	0.0	5.127561916513557	0.0
13	0.0	0.0	0.0	5.41241777538109	0.0
14	0.0	0.0	0.0	5.533724023591614	0.0
15	0.0	0.0	0.0	5.661862223012619	0.0
16	0.0	0.0	0.0	5.9183472310518965	0.0
17	0.0	0.0	0.0	6.214102920476609	0.0
18	0.0	0.0	0.0	6.725769129072247	0.0
19	0.0	0.0	0.0	6.908041415183933	0.0
20	0.0	0.0	0.0	7.128332727497456	0.0
21	0.0	0.0	0.0	7.346485795538997	0.0
22	0.0	0.0	0.0	7.552435225540441	0.0
23	0.0	0.0	0.0	7.789936796628458	0.0
24	0.0	0.0	0.0	7.95750213433285	0.0
25	5.215229931664842E-5	0.0	0.0	8.09069910678757	0.0
26	5.215229931664842E-5	0.0	0.0	8.235943260384435	0.0
27	5.215229931664842E-5	0.0	0.0	8.421396836754438	0.0
28	5.215229931664842E-5	0.0	0.0	8.568309863929436	0.0
29	5.215229931664842E-5	0.0	0.0	8.719707988845666	0.0
30	5.215229931664842E-5	0.0	0.0	8.921798148697679	0.0
31	1.0430459863329685E-4	0.0	0.0	9.100628383054467	0.0
32	1.0430459863329685E-4	0.0	0.0	9.254686275235846	0.0
33	1.0430459863329685E-4	0.0	0.0	9.410882411689208	0.0
34	1.0430459863329685E-4	0.0	0.0	9.570259838400885	0.0
35	1.0430459863329685E-4	0.0	0.0	9.762597518280685	0.0
36	1.0430459863329685E-4	0.0	0.0	9.927033718026077	0.0
37	1.0430459863329685E-4	0.0	0.0	10.086306840139121	0.0
38	1.0430459863329685E-4	0.0	0.0	10.251003801381097	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	25	4.443957E-5	44.0	22
CGTACTC	20	7.8578E-4	44.0	16
ATCATCG	20	7.8578E-4	44.0	36
TCACGAC	265	0.0	43.169815	25
CGGTCTA	260	0.0	43.153843	31
CGACGGT	270	0.0	42.37037	28
CGTTTTT	5400	0.0	41.555553	1
CACGACG	285	0.0	40.14035	26
TACTACG	55	7.8216544E-11	40.0	1
ACGAGTA	55	7.8216544E-11	40.0	5
TACGTTG	55	7.8216544E-11	40.0	1
TCTAGCG	105	0.0	39.809525	1
TATAGCG	100	0.0	39.6	1
ATGGTCC	790	0.0	39.26582	11
GAGCGAT	1400	0.0	39.12857	7
TACGTAG	125	0.0	38.72	1
TGTTGCG	165	0.0	38.666668	1
ACGAGAT	205	0.0	38.634148	5
GCGCGAC	890	0.0	38.5618	9
AGGGGAA	14745	0.0	38.524246	8
>>END_MODULE