FastQCFastQC Report
Thu 26 May 2016
SRR1546760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546760_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3344017
Sequences flagged as poor quality0
Sequence length50
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151630.45343669006467374No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92700.2772115093912501No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA58710.17556728928112508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA302.5291738E-644.02
CCCGTCG207.858642E-444.035
CGTCGGA254.4446708E-544.030
CGTTTTT237700.043.0281871
GAGCGAT23000.042.3739137
CGACGGT6450.040.93023728
AGCGATA4900.040.8571438
TCGCGCG603.6379788E-1240.3333321
CACGACG6550.040.30534426
CGGTCTA6700.040.059731
TCACGAC7000.039.28571725
TATACGA1400.039.2857132
ACGGTCT6800.039.14705730
TATGCGA2250.038.1333352
CGCGCGA750.038.133332
GTTGCGA1500.038.133332
CCGCCAC3350.038.0895540
AGCGATC2200.038.08
CTCACGA7250.037.93103424
CCGGTCG357.2943785E-637.71428714
Produced by FastQC (version 0.11.5)