FastQCFastQC Report
Thu 26 May 2016
SRR1546759_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546759_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3255280
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA75950.23331326337519354No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74400.22855176820427123No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45790.14066378314615025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA302.529152E-644.00000416
TACGACG551.8189894E-1244.01
CGTTTTT106050.042.7967951
GAGCGAT21200.040.575477
AGCGATA4850.040.371138
CGACGGT3400.040.1176528
GTAAGCG1000.039.61
TGTACGA1200.038.5000042
TTCGCGA750.038.133332
CGGTCTA3700.038.05405431
ACTCGCG357.294322E-637.71428710
TAATGCG2350.037.446811
CGAAGAG6550.037.2824443
GCGCGAC8400.036.9285749
CCGAACG301.301619E-436.6666681
AAGTACG2350.036.510641
AGACCGA6350.036.3779538
GAGGGGA206350.036.3237237
CGTTGAG2850.036.2807043
GCGATAC1700.036.2352949
Produced by FastQC (version 0.11.5)