##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546759_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3255280
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45300496424271	31.0	31.0	33.0	30.0	34.0
2	31.8409577670738	31.0	31.0	34.0	30.0	34.0
3	31.982993782408887	31.0	31.0	34.0	30.0	34.0
4	35.669962030915926	37.0	35.0	37.0	33.0	37.0
5	35.54801706765624	37.0	35.0	37.0	33.0	37.0
6	35.60295980683689	37.0	35.0	37.0	33.0	37.0
7	35.99296803961564	37.0	35.0	37.0	35.0	37.0
8	22.183178098350986	35.0	0.0	37.0	0.0	37.0
9	30.003227372146174	37.0	18.0	39.0	17.0	39.0
10	34.29027518370155	35.0	32.0	39.0	27.0	39.0
11	35.907225184930326	37.0	35.0	39.0	32.0	39.0
12	36.26121623946327	37.0	35.0	39.0	32.0	39.0
13	36.319141210587105	38.0	35.0	39.0	32.0	39.0
14	37.423605649898015	39.0	36.0	41.0	33.0	41.0
15	37.714359133469316	39.0	36.0	41.0	33.0	41.0
16	37.80425094001131	40.0	36.0	41.0	33.0	41.0
17	37.76534829569192	40.0	36.0	41.0	33.0	41.0
18	37.737868017497725	39.0	36.0	41.0	33.0	41.0
19	37.746351465926125	39.0	36.0	41.0	33.0	41.0
20	37.6379236194736	39.0	35.0	41.0	33.0	41.0
21	37.52058870511907	39.0	35.0	41.0	33.0	41.0
22	37.476884630507975	39.0	35.0	41.0	33.0	41.0
23	37.34763215453049	39.0	35.0	41.0	32.0	41.0
24	37.275963972377184	39.0	35.0	41.0	32.0	41.0
25	37.146074377626505	39.0	35.0	41.0	32.0	41.0
26	37.07552222850262	39.0	35.0	41.0	32.0	41.0
27	36.9466706396992	39.0	35.0	41.0	32.0	41.0
28	36.98632836499472	39.0	35.0	41.0	32.0	41.0
29	36.93440625691185	39.0	35.0	41.0	32.0	41.0
30	36.85675026418618	39.0	35.0	41.0	31.0	41.0
31	36.787785689710255	39.0	35.0	41.0	31.0	41.0
32	36.546088201322156	39.0	35.0	41.0	31.0	41.0
33	36.4533468703153	39.0	35.0	41.0	30.0	41.0
34	36.354060173011234	39.0	35.0	41.0	30.0	41.0
35	36.206422181809245	39.0	35.0	41.0	30.0	41.0
36	36.07620941977341	38.0	35.0	41.0	30.0	41.0
37	36.04740943943378	38.0	35.0	40.0	30.0	41.0
38	35.98581228035684	38.0	35.0	40.0	30.0	41.0
39	35.92815548892876	38.0	35.0	40.0	29.0	41.0
40	35.88637229362758	38.0	35.0	40.0	29.0	41.0
41	35.926307107222726	38.0	35.0	40.0	29.0	41.0
42	35.702561070015484	38.0	35.0	40.0	28.0	41.0
43	35.725952913420656	38.0	35.0	40.0	29.0	41.0
44	35.53472727384434	38.0	35.0	40.0	28.0	41.0
45	35.334180777076014	38.0	34.0	40.0	27.0	41.0
46	35.406625236538794	38.0	34.0	40.0	27.0	41.0
47	35.26636049740729	38.0	34.0	40.0	27.0	41.0
48	35.300536052198275	38.0	34.0	40.0	27.0	41.0
49	35.243184303654374	38.0	34.0	40.0	27.0	41.0
50	34.74545937676636	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	1.0
13	10.0
14	17.0
15	49.0
16	131.0
17	338.0
18	869.0
19	1938.0
20	3664.0
21	6316.0
22	9815.0
23	14866.0
24	21641.0
25	30273.0
26	39843.0
27	47040.0
28	52372.0
29	60841.0
30	75562.0
31	96266.0
32	125785.0
33	166010.0
34	258164.0
35	409652.0
36	310948.0
37	457469.0
38	542963.0
39	522431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.32407043326534	24.20406232336389	30.568307488142345	12.903559755228427
2	29.18968567988007	26.710759135926864	30.753176378068858	13.346378806124203
3	27.834379838293483	25.67376692634735	32.56162296330884	13.93023027205033
4	24.843577203804283	27.90349831658106	32.57931729375046	14.673607185864196
5	22.534344203878007	32.77251112039517	30.671462977071094	14.021681698655723
6	20.39839890884962	40.899523236096435	27.516926347349536	11.185151507704406
7	87.24807082647268	4.099524464869382	6.730050871200019	1.9223538374579143
8	54.63302695927846	39.66749404045121	3.667825809146986	2.031653191123344
9	83.0874149074734	4.945166007225185	8.533858838563809	3.4335602467376076
10	45.80748814234106	27.028765574697104	14.413015163058171	12.750731119903664
11	39.067361332972894	22.725295519893834	23.980118453712123	14.227224693421151
12	34.370130987196184	21.80380796736379	26.924012680936816	16.90204836450321
13	21.607757243616525	34.926826570986215	28.79958098842496	14.665835196972305
14	16.57716079722789	38.30134427760438	28.596157626993683	16.52533729817404
15	14.39114300459561	24.429019930697205	45.29238652281831	15.887450541888871
16	16.354414981199774	21.031247696050723	43.135244894448405	19.479092428301097
17	17.135945295028385	21.67555479098572	30.308483448428397	30.880016465557492
18	21.48693814356983	23.413838440932885	34.123946327197665	20.975277088299624
19	26.62136590400826	25.61687473888575	26.988799734585044	20.77295962252095
20	28.106368730186038	24.446990735051976	26.14503207097393	21.30160846378806
21	22.332395369983534	29.409636037453	28.051872649971738	20.20609594259173
22	22.339983042933326	25.16459413629549	26.613256002555847	25.882166818215328
23	18.869621046423042	30.758367943771347	26.27580423189403	24.096206777911576
24	19.556658720601607	25.261298567250744	35.47799267651323	19.704050035634417
25	18.062993045145117	26.450658622299773	33.96832223341771	21.5180260991374
26	17.809589344081	31.713063085203114	28.988535548401366	21.488812022314516
27	19.69852055737141	31.45121157012607	28.676672974367794	20.173594898134724
28	17.403940674842104	28.280885208031258	35.96004030375267	18.355133813373964
29	17.745908186085373	24.894479123147626	34.77123319652994	22.588379494237053
30	20.204928608291763	28.449134943845078	30.224926888009634	21.12100955985353
31	26.858887714728073	25.9817895849205	25.46536703447937	21.69395566587206
32	27.626778648841267	26.09059742940699	26.657430390012532	19.625193531739203
33	27.088975449116514	26.601982010764054	25.598043793467845	20.710998746651594
34	20.93021184045612	27.09041925732963	28.97600206433855	23.003366837875696
35	21.927821877073555	25.848867071342557	30.00983018357868	22.21348086800521
36	28.464955395542013	24.915183947310215	27.369043523137794	19.250817134009978
37	22.771558821360987	30.355852645548154	28.373135337052418	18.499453196038438
38	21.785714285714285	30.823707945245875	26.272087193728343	21.118490575311494
39	21.079968543412548	31.092317711533262	27.317680813939198	20.51003293111499
40	23.224054459217026	26.193967953601533	27.185157651569142	23.3968199356123
41	18.317349045243418	25.692597871765255	27.736876704922466	28.25317637806886
42	22.434014892728122	26.16957681059694	26.45692536433118	24.93948293234376
43	22.687295716497506	26.481500823277877	27.08209432061144	23.749109139613182
44	21.38399768990686	29.201266864908703	28.389232262662507	21.025503182521934
45	19.47218058047234	32.66966282470325	25.625138236956573	22.233018357867834
46	23.68416848934654	29.48965987564818	26.906748421026762	19.919423213978522
47	22.138679314836203	26.43376299427392	27.96631933351355	23.461238357376324
48	22.945553070703596	24.562526111425132	29.50016588434789	22.991754933523385
49	22.344713818780566	23.37067779115775	30.70350323167285	23.581105158388834
50	20.971652208104985	28.6387960482662	28.024286697304074	22.36526504632474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1551.0
1	2193.5
2	2836.0
3	11971.5
4	21107.0
5	15752.0
6	10397.0
7	11293.5
8	12190.0
9	13201.5
10	14213.0
11	14536.5
12	14860.0
13	14653.5
14	14447.0
15	13845.0
16	13243.0
17	12952.5
18	12662.0
19	12826.0
20	12990.0
21	14052.5
22	15115.0
23	17403.5
24	19692.0
25	22182.5
26	24673.0
27	29653.0
28	34633.0
29	40891.5
30	47150.0
31	53657.0
32	60164.0
33	68863.0
34	77562.0
35	86863.5
36	96165.0
37	110768.5
38	125372.0
39	156526.5
40	187681.0
41	227243.5
42	266806.0
43	291717.0
44	316628.0
45	331778.5
46	346929.0
47	338064.0
48	329199.0
49	310395.5
50	291592.0
51	257909.5
52	224227.0
53	198378.0
54	172529.0
55	152009.0
56	131489.0
57	118807.5
58	106126.0
59	95627.5
60	85129.0
61	73824.0
62	62519.0
63	51328.5
64	40138.0
65	32014.0
66	23890.0
67	19489.0
68	15088.0
69	12802.0
70	10516.0
71	8560.5
72	6605.0
73	5129.5
74	3654.0
75	2671.5
76	1689.0
77	1394.0
78	1099.0
79	755.0
80	411.0
81	304.5
82	198.0
83	133.5
84	69.0
85	45.0
86	21.0
87	16.5
88	12.0
89	11.0
90	10.0
91	6.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3255280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.41886382646028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6061120394449	19.19471945818685
2	7.53871092020585	3.681735107751115
3	2.597193007401541	1.9026150713631922
4	1.361070130127943	1.3294314466342727
5	0.8673334185683266	1.0589648320079124
6	0.6848371217176898	1.0033766651117564
7	0.5305628875822501	0.9069020032271163
8	0.4381980441970112	0.8560238696214433
9	0.3592895818199826	0.7896099035455214
>10	5.166550923629815	30.86941792929464
>50	1.489377298032118	25.18855276067078
>100	0.35656187503350656	11.756257364498591
>500	0.002847025710380672	0.47698118388999156
>1k	0.0010845812230021604	0.48767419627175623
>5k	2.711453057505401E-4	0.4977382079251819
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7595	0.23331326337519354	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7440	0.22855176820427123	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4579	0.14066378314615025	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06776682804551375	0.0
2	0.0	0.0	0.0	0.22526480057015064	0.0
3	0.0	0.0	0.0	0.337943279840751	0.0
4	0.0	0.0	0.0	0.5001413088889435	0.0
5	0.0	0.0	0.0	0.8116659703619965	0.0
6	0.0	0.0	0.0	1.2713192106362585	0.0
7	0.0	0.0	0.0	1.580048413654125	0.0
8	0.0	0.0	0.0	2.493917573910693	0.0
9	0.0	0.0	0.0	3.2843564916074808	0.0
10	0.0	0.0	0.0	4.419957730210612	0.0
11	0.0	0.0	0.0	5.03336118552014	0.0
12	0.0	0.0	0.0	5.635613526332604	0.0
13	0.0	0.0	0.0	5.906834435133076	0.0
14	0.0	0.0	0.0	6.0098977660907815	0.0
15	0.0	0.0	0.0	6.138980364208302	0.0
16	3.071932368336979E-5	0.0	0.0	6.389895800054066	0.0
17	3.071932368336979E-5	0.0	0.0	6.686920940748569	0.0
18	3.071932368336979E-5	0.0	0.0	7.159844928854047	0.0
19	3.071932368336979E-5	0.0	0.0	7.359919884003833	0.0
20	3.071932368336979E-5	0.0	0.0	7.5953835000368635	0.0
21	3.071932368336979E-5	0.0	0.0	7.8457152687326435	3.071932368336979E-5
22	3.071932368336979E-5	0.0	0.0	8.105170676562384	3.071932368336979E-5
23	3.071932368336979E-5	0.0	0.0	8.402533729817405	3.071932368336979E-5
24	3.071932368336979E-5	0.0	0.0	8.607769531345998	3.071932368336979E-5
25	3.071932368336979E-5	0.0	0.0	8.784159887935907	3.071932368336979E-5
26	3.071932368336979E-5	0.0	0.0	8.954191344523359	3.071932368336979E-5
27	3.071932368336979E-5	0.0	0.0	9.152238824310045	3.071932368336979E-5
28	3.071932368336979E-5	0.0	0.0	9.333820746602443	3.071932368336979E-5
29	6.143864736673958E-5	0.0	0.0	9.522529551989383	3.071932368336979E-5
30	6.143864736673958E-5	0.0	0.0	9.7890504042663	3.071932368336979E-5
31	6.143864736673958E-5	0.0	0.0	10.003532722223587	3.071932368336979E-5
32	6.143864736673958E-5	0.0	0.0	10.197156619399868	3.071932368336979E-5
33	9.215797105010936E-5	0.0	0.0	10.396309994839154	3.071932368336979E-5
34	9.215797105010936E-5	0.0	0.0	10.597152933081025	3.071932368336979E-5
35	9.215797105010936E-5	0.0	0.0	10.832401513848271	3.071932368336979E-5
36	9.215797105010936E-5	0.0	0.0	11.037207244845298	3.071932368336979E-5
37	9.215797105010936E-5	0.0	0.0	11.2505529478263	3.071932368336979E-5
38	9.215797105010936E-5	0.0	0.0	11.463530018923104	3.071932368336979E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGAA	30	2.529152E-6	44.000004	16
TACGACG	55	1.8189894E-12	44.0	1
CGTTTTT	10605	0.0	42.796795	1
GAGCGAT	2120	0.0	40.57547	7
AGCGATA	485	0.0	40.37113	8
CGACGGT	340	0.0	40.11765	28
GTAAGCG	100	0.0	39.6	1
TGTACGA	120	0.0	38.500004	2
TTCGCGA	75	0.0	38.13333	2
CGGTCTA	370	0.0	38.054054	31
ACTCGCG	35	7.294322E-6	37.714287	10
TAATGCG	235	0.0	37.44681	1
CGAAGAG	655	0.0	37.282444	3
GCGCGAC	840	0.0	36.928574	9
CCGAACG	30	1.301619E-4	36.666668	1
AAGTACG	235	0.0	36.51064	1
AGACCGA	635	0.0	36.377953	8
GAGGGGA	20635	0.0	36.323723	7
CGTTGAG	285	0.0	36.280704	3
GCGATAC	170	0.0	36.235294	9
>>END_MODULE