FastQCFastQC Report
Thu 26 May 2016
SRR1546758_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546758_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2100325
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA73000.347565257757728No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42570.20268291811981481No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.12664706652541868No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA207.8579696E-444.00000415
CCACGTA207.8579696E-444.00000429
CCTTACG254.444103E-544.030
CCGTCGA254.444103E-544.02
TATCTCG302.5287209E-644.01
CATACCG254.444103E-544.01
TATAGCG1350.044.01
ACGAGTT254.444103E-544.05
TAGTCGA302.5287209E-644.02
TCGCGAA254.444103E-544.021
TAATGCG1050.043.9999961
TTGTGCG1100.042.0000041
CGTTTTT67800.041.8908541
GAGCGAT13400.041.8656737
TAAGCGA750.041.0666662
CGACGGT2950.040.2711928
TCGTAGA950.039.368422
ATGTACG452.352499E-839.111111
TATACGA452.352499E-839.111112
CACGACG3050.038.9508226
Produced by FastQC (version 0.11.5)