##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546758_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2100325
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41063025960268	31.0	31.0	33.0	30.0	34.0
2	31.809163343768226	31.0	31.0	34.0	30.0	34.0
3	31.94333686453287	31.0	31.0	34.0	30.0	34.0
4	35.64018901836621	37.0	35.0	37.0	33.0	37.0
5	35.52141501910419	37.0	35.0	37.0	33.0	37.0
6	35.56563341387643	37.0	35.0	37.0	33.0	37.0
7	35.97106066918215	37.0	35.0	37.0	35.0	37.0
8	22.146651113518146	35.0	0.0	37.0	0.0	37.0
9	29.968814826276887	37.0	18.0	39.0	17.0	39.0
10	34.214002594836515	35.0	32.0	39.0	27.0	39.0
11	35.86567412186209	37.0	35.0	39.0	32.0	39.0
12	36.15553069167867	37.0	35.0	39.0	32.0	39.0
13	36.18294978158142	38.0	35.0	39.0	32.0	39.0
14	37.26848606763239	39.0	35.0	41.0	32.0	41.0
15	37.587183888207775	39.0	35.0	41.0	33.0	41.0
16	37.68145834573221	39.0	35.0	41.0	33.0	41.0
17	37.64659421756157	39.0	35.0	41.0	33.0	41.0
18	37.61932796114887	39.0	36.0	41.0	33.0	41.0
19	37.62205182531275	39.0	36.0	41.0	33.0	41.0
20	37.49917798435956	39.0	35.0	41.0	33.0	41.0
21	37.407455036720506	39.0	35.0	41.0	32.0	41.0
22	37.35376477449918	39.0	35.0	41.0	32.0	41.0
23	37.22306881077928	39.0	35.0	41.0	32.0	41.0
24	37.15427374334924	39.0	35.0	41.0	32.0	41.0
25	37.02777903419709	39.0	35.0	41.0	32.0	41.0
26	36.94895170985443	39.0	35.0	41.0	32.0	41.0
27	36.812898479997145	38.0	35.0	41.0	31.0	41.0
28	36.86703057860093	39.0	35.0	41.0	32.0	41.0
29	36.80609524716413	38.0	35.0	40.0	31.0	41.0
30	36.72375180031662	38.0	35.0	40.0	31.0	41.0
31	36.648015902300834	38.0	35.0	40.0	31.0	41.0
32	36.396961898753766	38.0	35.0	40.0	30.0	41.0
33	36.26698439527216	38.0	35.0	41.0	30.0	41.0
34	36.204395034101864	38.0	35.0	41.0	30.0	41.0
35	36.06084296477926	38.0	35.0	41.0	30.0	41.0
36	35.92080082844322	38.0	35.0	40.0	30.0	41.0
37	35.85832716365324	38.0	35.0	40.0	29.0	41.0
38	35.80765357742254	38.0	35.0	40.0	29.0	41.0
39	35.75319152988228	38.0	35.0	40.0	29.0	41.0
40	35.67858355254544	38.0	35.0	40.0	29.0	41.0
41	35.71506409722305	38.0	35.0	40.0	29.0	41.0
42	35.48266863461607	38.0	35.0	40.0	27.0	41.0
43	35.50124956851916	38.0	35.0	40.0	28.0	41.0
44	35.30325116351041	38.0	34.0	40.0	27.0	41.0
45	35.093641698308595	38.0	34.0	40.0	26.0	41.0
46	35.17465582707438	38.0	34.0	40.0	27.0	41.0
47	35.04777165438682	38.0	34.0	40.0	26.0	41.0
48	35.08307571447276	38.0	34.0	40.0	26.0	41.0
49	35.0323773701689	37.0	34.0	40.0	26.0	41.0
50	34.505396545772676	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	7.0
14	9.0
15	28.0
16	77.0
17	235.0
18	636.0
19	1454.0
20	2891.0
21	4764.0
22	7554.0
23	11079.0
24	15422.0
25	21240.0
26	27340.0
27	31845.0
28	34939.0
29	40530.0
30	50117.0
31	63483.0
32	82339.0
33	109112.0
34	172692.0
35	278587.0
36	197918.0
37	287316.0
38	341199.0
39	317508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.68414412055277	25.45582231321343	30.468808398700205	12.391225167533596
2	29.741063882970494	26.565936224155784	30.62940259245593	13.063597300417792
3	28.067560972706605	25.82471760322807	32.454643924154595	13.65307749991073
4	24.410793567662147	28.85753395307869	32.20949138823753	14.522181091021627
5	23.210598359777652	32.03366145715544	31.26911311344673	13.486627069620177
6	19.801411686286645	41.56680513730018	27.962672443550403	10.669110732862771
7	86.53575041957792	4.643186173568377	7.005963362812897	1.8151000440408034
8	54.16028471784129	40.152595431659385	3.6786687774511084	2.008451073048219
9	82.33482913358647	5.340411602966207	8.73126777998643	3.593491483460893
10	48.03451846738005	24.052134788663658	15.426422101341458	12.486924642614833
11	42.0071655577113	23.306250223179745	20.727077952221677	13.959506266887267
12	36.1930177472534	22.392582100389223	25.526811326818468	15.887588825538904
13	21.779915013152724	38.43238546415436	26.409150964731648	13.378548557961267
14	15.8988251818171	41.66021925178246	27.38818992298811	15.05276564341233
15	14.119433897134966	24.440455643769415	46.60745360837013	14.832656850725485
16	15.03262590313404	20.43678954447526	44.51358718293598	20.016997369454728
17	16.402890028924094	21.324128408698652	29.269089307607153	33.00389225477009
18	20.979848356801924	23.63734183995334	34.65806482330115	20.72474497994358
19	27.21007463130706	26.858033875709715	26.172520919381526	19.759370573601704
20	28.64885196338662	25.008986704438595	25.52371656767405	20.818444764500732
21	21.38345256091319	30.315832073607655	27.4670824753312	20.833632890147953
22	21.277230904740932	26.489281420732507	25.93317700832014	26.30031066620642
23	18.594265173247	31.811552973944508	24.96737409686596	24.626807755942533
24	19.631199933343648	25.94817468725078	35.568304905193244	18.852320474212327
25	16.794924595003156	27.229643031435614	34.62235606394248	21.35307630961875
26	16.744837108542725	33.36688369657077	29.3111304202921	20.57714877459441
27	19.187935200504683	33.039077285658166	28.630997583707284	19.14198993012986
28	16.650089867044386	28.794972206682303	37.085593896182736	17.469344030090582
29	17.18595931582017	25.790913311035197	35.29820384940426	21.724923523740376
30	19.45346553509576	30.644543106424006	30.426814897694403	19.475176460785832
31	27.48346089295704	27.454227321962076	24.382702676966662	20.67960910811422
32	29.33826907740469	27.34114958399295	25.575422851225404	17.74515848737695
33	26.5863140228298	29.02241319795746	24.740980562531988	19.65029221668075
34	21.08797448013998	28.344708557009035	27.61820194493709	22.949115017913893
35	21.1362527227929	27.31696285098735	29.360218061490485	22.18656636472927
36	29.300703462559362	26.222894075916827	25.61346458286218	18.862937878661636
37	21.2136693130825	31.975146703486367	28.68632235487365	18.124861628557483
38	20.418363824646185	32.41198385964077	24.668848868627475	22.50080344708557
39	20.322616737885806	31.476938092914192	27.11870781902801	21.081737350172
40	22.951638436908574	26.872698272886343	25.722971444895432	24.452691845309655
41	17.269660647756897	25.48000904621904	27.31124944948996	29.939080856534105
42	22.34692250008927	26.423291633437685	25.354218989918227	25.875566876554817
43	22.008641519764797	27.523597538476185	26.35235023151179	24.11541071024722
44	21.16620046897504	30.527608822444147	27.98962065394641	20.316570054634404
45	18.59478890171759	34.302976920238535	24.4951614631069	22.607072714936972
46	22.664397176627425	31.858926594693678	25.93246283313297	19.544213395545928
47	21.77039267732375	26.87931629628748	27.727851642007785	23.62243938438099
48	22.44766881315987	24.6597550379108	29.44734743432564	23.44522871460369
49	21.356218680442314	23.806934641067453	30.940306857272088	23.896539821218145
50	19.9011581540952	30.298834704152927	27.548355611631532	22.251651530120338
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1272.0
1	1637.5
2	2003.0
3	7068.0
4	12133.0
5	9478.5
6	6824.0
7	7489.0
8	8154.0
9	8980.5
10	9807.0
11	10393.5
12	10980.0
13	10843.5
14	10707.0
15	10401.0
16	10095.0
17	9744.0
18	9393.0
19	9317.5
20	9242.0
21	9551.0
22	9860.0
23	10543.0
24	11226.0
25	13387.5
26	15549.0
27	18258.5
28	20968.0
29	25301.0
30	29634.0
31	34255.0
32	38876.0
33	43467.0
34	48058.0
35	55003.5
36	61949.0
37	72245.0
38	82541.0
39	105803.0
40	129065.0
41	157776.5
42	186488.0
43	202529.0
44	218570.0
45	223584.0
46	228598.0
47	223287.5
48	217977.0
49	205801.5
50	193626.0
51	169453.5
52	145281.0
53	125764.5
54	106248.0
55	90691.5
56	75135.0
57	66770.5
58	58406.0
59	51555.0
60	44704.0
61	38259.5
62	31815.0
63	26410.5
64	21006.0
65	17087.5
66	13169.0
67	10886.0
68	8603.0
69	7145.5
70	5688.0
71	4249.0
72	2810.0
73	2267.0
74	1724.0
75	1390.0
76	1056.0
77	826.5
78	597.0
79	438.0
80	279.0
81	208.0
82	137.0
83	91.5
84	46.0
85	29.0
86	12.0
87	11.0
88	10.0
89	6.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2100325.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.664419180693315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64123746406698	20.702585014496318
2	7.334037404198443	3.9111569526486236
3	2.5485880806341057	2.0386986270283924
4	1.5153855207221805	1.6162749897955777
5	1.0537426177711455	1.404871743440546
6	0.7813715385055191	1.2500890943144647
7	0.6545015647317254	1.2216332853498486
8	0.5327999666454838	1.1365441320075678
9	0.45885711257455525	1.1011642554357473
>10	6.228889398979704	39.05142581095571
>50	1.0934880971780871	19.547294010887942
>100	0.15401729469219613	5.757847437506524
>500	0.0019954901352609604	0.3920021603507246
>1k	9.07040970573164E-4	0.5153009642418891
>5k	1.814081941146328E-4	0.353111521540016
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	7300	0.347565257757728	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4257	0.20268291811981481	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2660	0.12664706652541868	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07108423696332711	0.0
2	0.0	0.0	0.0	0.24591432278337877	0.0
3	0.0	0.0	0.0	0.3697523002392487	0.0
4	0.0	0.0	0.0	0.5446299977384452	0.0
5	0.0	0.0	0.0	0.8888624379560306	0.0
6	0.0	0.0	0.0	1.3468391796507684	0.0
7	0.0	0.0	0.0	1.65679121088403	0.0
8	0.0	0.0	0.0	2.5415590444335994	0.0
9	0.0	0.0	0.0	3.2865865996929045	0.0
10	0.0	0.0	0.0	4.468546534464904	0.0
11	0.0	0.0	0.0	5.194100912953948	0.0
12	0.0	0.0	0.0	5.941175770416483	0.0
13	4.761167914489424E-5	0.0	0.0	6.251651530120339	0.0
14	4.761167914489424E-5	0.0	0.0	6.38377393974742	0.0
15	4.761167914489424E-5	0.0	0.0	6.544796638615453	0.0
16	4.761167914489424E-5	0.0	0.0	6.869793960458501	0.0
17	4.761167914489424E-5	0.0	0.0	7.2409269993929515	0.0
18	4.761167914489424E-5	0.0	0.0	7.842357730351255	0.0
19	4.761167914489424E-5	0.0	0.0	8.094318736386036	0.0
20	4.761167914489424E-5	0.0	0.0	8.367276492923715	0.0
21	4.761167914489424E-5	0.0	0.0	8.655517598466904	0.0
22	4.761167914489424E-5	0.0	0.0	8.94342542225608	0.0
23	4.761167914489424E-5	0.0	0.0	9.2878006975111	0.0
24	4.761167914489424E-5	0.0	0.0	9.52157404211253	0.0
25	4.761167914489424E-5	0.0	0.0	9.715401187911395	0.0
26	4.761167914489424E-5	0.0	0.0	9.90613357456584	0.0
27	4.761167914489424E-5	0.0	0.0	10.117196148215157	0.0
28	9.522335828978848E-5	0.0	0.0	10.321497863425899	0.0
29	9.522335828978848E-5	0.0	0.0	10.526847035577827	0.0
30	9.522335828978848E-5	0.0	0.0	10.810803089997977	0.0
31	9.522335828978848E-5	0.0	0.0	11.05052789449252	0.0
32	9.522335828978848E-5	0.0	0.0	11.260876292954661	0.0
33	9.522335828978848E-5	0.0	0.0	11.47712853963077	0.0
34	9.522335828978848E-5	0.0	0.0	11.69638032209301	0.0
35	9.522335828978848E-5	0.0	0.0	11.946436860961994	0.0
36	9.522335828978848E-5	0.0	0.0	12.173068453691691	0.0
37	9.522335828978848E-5	0.0	0.0	12.40079511504172	0.0
38	9.522335828978848E-5	0.0	0.0	12.631664147215313	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGTA	20	7.8579696E-4	44.000004	15
CCACGTA	20	7.8579696E-4	44.000004	29
CCTTACG	25	4.444103E-5	44.0	30
CCGTCGA	25	4.444103E-5	44.0	2
TATCTCG	30	2.5287209E-6	44.0	1
CATACCG	25	4.444103E-5	44.0	1
TATAGCG	135	0.0	44.0	1
ACGAGTT	25	4.444103E-5	44.0	5
TAGTCGA	30	2.5287209E-6	44.0	2
TCGCGAA	25	4.444103E-5	44.0	21
TAATGCG	105	0.0	43.999996	1
TTGTGCG	110	0.0	42.000004	1
CGTTTTT	6780	0.0	41.890854	1
GAGCGAT	1340	0.0	41.865673	7
TAAGCGA	75	0.0	41.066666	2
CGACGGT	295	0.0	40.27119	28
TCGTAGA	95	0.0	39.36842	2
ATGTACG	45	2.352499E-8	39.11111	1
TATACGA	45	2.352499E-8	39.11111	2
CACGACG	305	0.0	38.95082	26
>>END_MODULE