FastQCFastQC Report
Thu 26 May 2016
SRR1546757_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546757_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2187457
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA61920.2830684214592561No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53330.2437990781075925No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.1476600454317502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCCG351.4468787E-744.026
CGACCGA302.52877E-644.032
TCGATCT207.8580435E-444.012
TATAGCG1250.044.01
CGTCCGT207.8580435E-444.028
TTCGATC302.52877E-644.031
ATTAGCG1700.042.705881
CGTTTTT76850.042.5686381
AGCGATA2700.042.370378
GAGCGAT14600.042.0410927
TTAGCGA1950.041.7435872
TTCGAAG1450.040.9655151
CGGTCTA4250.040.8941231
CCGAGAC557.8216544E-1140.0000045
GCGTAAG2450.039.5102041
TCACGAC4350.039.44827725
CGAACGG950.039.368422
CGAGACC2250.039.111116
TGTTACG850.038.823531
TGTAGCG1650.038.6666681
Produced by FastQC (version 0.11.5)