##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546757_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2187457
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494536349743104	31.0	31.0	33.0	30.0	34.0
2	31.886407824245232	31.0	31.0	34.0	30.0	34.0
3	32.02614954259672	33.0	31.0	34.0	30.0	34.0
4	35.70621091066019	37.0	35.0	37.0	33.0	37.0
5	35.59345669423445	37.0	35.0	37.0	33.0	37.0
6	35.6434723059699	37.0	35.0	37.0	33.0	37.0
7	36.03600482203765	37.0	35.0	37.0	35.0	37.0
8	21.893411847638603	35.0	0.0	37.0	0.0	37.0
9	29.862624956742007	37.0	18.0	39.0	17.0	39.0
10	34.33272745475683	35.0	32.0	39.0	27.0	39.0
11	35.941411876896325	37.0	35.0	39.0	32.0	39.0
12	36.376435285356465	37.0	35.0	39.0	32.0	39.0
13	36.472505288103946	39.0	35.0	39.0	32.0	39.0
14	37.60382215513265	39.0	36.0	41.0	33.0	41.0
15	37.869794011950866	40.0	36.0	41.0	33.0	41.0
16	37.93591599743446	40.0	36.0	41.0	33.0	41.0
17	37.901908014648974	40.0	36.0	41.0	33.0	41.0
18	37.86621999883884	40.0	36.0	41.0	33.0	41.0
19	37.88507339801422	40.0	36.0	41.0	33.0	41.0
20	37.790122045827644	40.0	36.0	41.0	33.0	41.0
21	37.69770651491664	40.0	35.0	41.0	33.0	41.0
22	37.66662658968839	39.0	35.0	41.0	33.0	41.0
23	37.53315333741418	39.0	35.0	41.0	33.0	41.0
24	37.44942963450253	39.0	35.0	41.0	33.0	41.0
25	37.323535045488896	39.0	35.0	41.0	32.0	41.0
26	37.263660497097774	39.0	35.0	41.0	32.0	41.0
27	37.143017211309754	39.0	35.0	41.0	32.0	41.0
28	37.19071643465449	39.0	35.0	41.0	32.0	41.0
29	37.12452359063515	39.0	35.0	41.0	32.0	41.0
30	37.048673413923105	39.0	35.0	41.0	32.0	41.0
31	36.98285909163015	39.0	35.0	41.0	32.0	41.0
32	36.76214069579425	39.0	35.0	41.0	31.0	41.0
33	36.64742255504908	39.0	35.0	41.0	31.0	41.0
34	36.55833828962123	39.0	35.0	41.0	30.0	41.0
35	36.40917558608009	39.0	35.0	41.0	30.0	41.0
36	36.268166185666736	39.0	35.0	41.0	30.0	41.0
37	36.21191685139411	39.0	35.0	41.0	30.0	41.0
38	36.12399603740782	39.0	35.0	41.0	30.0	41.0
39	36.03942523213028	39.0	35.0	41.0	30.0	41.0
40	35.95199814213491	39.0	35.0	40.0	29.0	41.0
41	35.97338964834509	39.0	35.0	40.0	29.0	41.0
42	35.74628026973787	38.0	35.0	40.0	28.0	41.0
43	35.80631619272973	38.0	35.0	40.0	29.0	41.0
44	35.61656709137597	38.0	35.0	40.0	28.0	41.0
45	35.45942937392598	38.0	35.0	40.0	27.0	41.0
46	35.563442389953266	38.0	35.0	40.0	28.0	41.0
47	35.45232569143074	38.0	35.0	40.0	27.0	41.0
48	35.47364679625702	38.0	35.0	40.0	28.0	41.0
49	35.409888285804016	38.0	35.0	40.0	27.0	41.0
50	34.905830377465705	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	1.0
13	1.0
14	8.0
15	22.0
16	85.0
17	212.0
18	536.0
19	1166.0
20	2409.0
21	4034.0
22	6480.0
23	9509.0
24	13983.0
25	20139.0
26	26411.0
27	30908.0
28	33816.0
29	39068.0
30	48116.0
31	61299.0
32	80633.0
33	107729.0
34	166752.0
35	258754.0
36	208331.0
37	310850.0
38	377739.0
39	378462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.552848810285184	24.578265995628716	31.081799550802597	12.787085643283502
2	29.34489683682925	26.70329062468428	31.13912639197022	12.812686146516253
3	27.457088299335712	25.785055431946773	33.26620820432127	13.491648064396236
4	24.471155318710263	27.99922467047352	32.85916020292056	14.670459807895654
5	23.18107281651708	32.587657723100385	30.453032905332538	13.778236555049997
6	20.110383884117493	41.26284539536091	27.436287890459106	11.19048283006249
7	87.85374981085342	3.8046462170456383	6.661570947451767	1.6800330246491701
8	54.21532857560172	40.58237487639757	3.5510183743040438	1.6512781736966713
9	83.25850519575928	5.702649240647931	8.10777080418038	2.9310747594124136
10	42.88747161658492	34.818467288728414	12.237634842650621	10.05642625203604
11	34.8312675403448	24.069455993877824	27.586599416582818	13.512677049194565
12	32.708711531243814	23.907441380561995	27.901805612636043	15.482041475558148
13	21.748221793616974	35.135547807339755	28.533132308429376	14.583098090613897
14	16.65088730887053	38.32966773746867	28.941003183148283	16.078441770512516
15	14.700631829562822	25.614446363974242	44.496920396606654	15.188001409856286
16	17.04417503978364	23.2624001294654	41.42746577418436	18.265959056566597
17	17.76222344027791	22.94125095944743	30.15698137151953	29.139544228755128
18	20.79122012455559	24.93205580726844	34.50815261740002	19.768571450775944
19	25.54715361261958	26.335694827372606	28.422638707869456	19.694512852138352
20	27.31834271485108	25.916029435092895	26.80290401137028	19.962723838685744
21	22.192527670258205	29.010078826692364	29.2436834186912	19.55371008435823
22	22.075313937599688	25.74382033566831	27.893348303532367	24.287517423199635
23	18.219558144457242	31.057296211994114	27.970378389152334	22.752767254396314
24	18.943549518916257	26.113061879616378	36.021462364745915	18.921926236721454
25	17.15238288112635	27.971795559867008	34.44200274565397	20.433818813352676
26	17.628963677914584	32.95996218439951	29.49278545818272	19.918288679503185
27	18.532295720555876	31.74745835003842	30.296275538216292	19.423970391189403
28	16.312914951013894	28.91238547774882	36.60922248985923	18.165477081378057
29	17.04079211614217	25.93660126804778	35.762120123961296	21.260486491848756
30	19.199188829768996	29.503574241687954	31.63961623017047	19.657620698372586
31	24.742520652977408	27.65494361717739	26.80505262503446	20.797483104810745
32	25.268108127382614	28.23488644576785	28.230726363992524	18.26627906285701
33	24.618495357851604	28.848795656326043	26.953032676756617	19.579676309065732
34	19.31868832164472	28.793891719928666	29.316141985876754	22.57127797254986
35	20.408949753069432	27.680818411516206	30.13133515310244	21.778896682311927
36	26.181268934657915	26.807612675357735	27.559536027451053	19.451582362533298
37	21.569246846909447	31.38617124816625	28.526915043358564	18.517666861565736
38	21.569566853199856	31.96647979823146	25.39423632098825	21.069717027580428
39	21.656654279375548	30.951511275421645	27.529912587996012	19.861921857206795
40	23.547160012745394	27.149379393514934	27.41480175381733	21.88865883992234
41	19.54214414271915	26.310277184877233	27.686807100665295	26.460771571738324
42	22.51710547910199	27.22791808021826	26.45254283855637	23.80243360212338
43	22.25666607389311	27.294250812701687	27.83474143720311	22.614341676202095
44	20.44337328688061	29.980840766241347	28.628357037418333	20.947428909459706
45	18.746197068102365	32.98944847830151	26.398004623633746	21.866349829962374
46	22.139498056418937	30.781222213739518	27.519855247440294	19.559424482401255
47	21.610481943187914	27.356240602672415	28.488285712587725	22.544991741551947
48	22.18247032970248	25.758769200948866	29.833683587837385	22.22507688151127
49	22.069325248450596	24.116405488199312	30.982323309669628	22.83194595368046
50	19.99138725926955	30.08310563361931	28.238543660515386	21.68696344659575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1321.0
1	1729.0
2	2137.0
3	8812.0
4	15487.0
5	11748.5
6	8010.0
7	8583.5
8	9157.0
9	9968.0
10	10779.0
11	11034.0
12	11289.0
13	11215.5
14	11142.0
15	10993.5
16	10845.0
17	10444.0
18	10043.0
19	9977.0
20	9911.0
21	11081.0
22	12251.0
23	13813.0
24	15375.0
25	17495.5
26	19616.0
27	23828.5
28	28041.0
29	33712.5
30	39384.0
31	46204.5
32	53025.0
33	59350.0
34	65675.0
35	73945.0
36	82215.0
37	92576.5
38	102938.0
39	124140.5
40	145343.0
41	169662.0
42	193981.0
43	207399.0
44	220817.0
45	223190.5
46	225564.0
47	219240.5
48	212917.0
49	201125.5
50	189334.0
51	164924.5
52	140515.0
53	120554.0
54	100593.0
55	87243.5
56	73894.0
57	65028.0
58	56162.0
59	48773.0
60	41384.0
61	34996.5
62	28609.0
63	23036.0
64	17463.0
65	13275.0
66	9087.0
67	7073.0
68	5059.0
69	4125.5
70	3192.0
71	2628.0
72	2064.0
73	1816.0
74	1568.0
75	1172.0
76	776.0
77	516.0
78	256.0
79	189.5
80	123.0
81	98.0
82	73.0
83	47.5
84	22.0
85	18.5
86	15.0
87	9.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2187457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.60728480089274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.59338429091245	20.379419896925317
2	6.872742976804723	3.6573005949435724
3	2.664873848414227	2.1271517232962522
4	1.5475011155390679	1.6469921164338879
5	1.0950728861846493	1.4568458080225282
6	0.8489975444344479	1.3553711676015565
7	0.7155614299761607	1.3327402731936844
8	0.6482137496742766	1.3797766279550454
9	0.5502856387706403	1.317744604135045
>10	7.499880197341498	46.08302321201918
>50	0.889975854731084	15.447447172094957
>100	0.07103663681589234	2.7350619311945614
>500	0.0014136173719556605	0.22943637209219409
>1k	7.068086859778303E-4	0.30983313362449977
>5k	3.5340434298891514E-4	0.5418553664677329
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	6192	0.2830684214592561	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5333	0.2437990781075925	No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3230	0.1476600454317502	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06107548628384467	0.0
2	0.0	0.0	0.0	0.19191234387693107	0.0
3	0.0	0.0	0.0	0.29010855984826217	0.0
4	0.0	0.0	0.0	0.42281973999946054	0.0
5	0.0	0.0	0.0	0.6891564039887412	0.0
6	0.0	0.0	0.0	1.0619637323156523	0.0
7	0.0	0.0	0.0	1.3089171581429944	0.0
8	0.0	0.0	0.0	2.009365212664752	0.0
9	0.0	0.0	0.0	2.666200981322147	0.0
10	0.0	0.0	0.0	3.6328485542801525	0.0
11	0.0	0.0	0.0	4.238940468315492	0.0
12	0.0	0.0	0.0	4.829489219673804	0.0
13	0.0	0.0	0.0	5.095185871082266	0.0
14	0.0	0.0	0.0	5.2063194842230045	0.0
15	0.0	0.0	0.0	5.344196480205096	0.0
16	0.0	0.0	0.0	5.616247542237402	0.0
17	0.0	0.0	0.0	5.947591198364128	0.0
18	0.0	0.0	0.0	6.4780701974941675	0.0
19	0.0	0.0	0.0	6.6884057606618095	0.0
20	0.0	0.0	0.0	6.940753578241766	0.0
21	0.0	0.0	0.0	7.194792857642459	0.0
22	0.0	0.0	0.0	7.465198173038373	0.0
23	0.0	0.0	0.0	7.773135654780871	0.0
24	0.0	0.0	0.0	7.989368476728914	0.0
25	0.0	0.0	0.0	8.170034885257174	0.0
26	0.0	0.0	0.0	8.346495496825765	0.0
27	0.0	0.0	0.0	8.532464866737952	0.0
28	0.0	0.0	0.0	8.726800115385124	0.0
29	0.0	0.0	0.0	8.927626920209175	0.0
30	0.0	0.0	0.0	9.209689607612859	0.0
31	0.0	0.0	0.0	9.447865718046115	0.0
32	0.0	0.0	0.0	9.664738552574976	0.0
33	0.0	0.0	0.0	9.875988419429502	0.0
34	0.0	0.0	0.0	10.084038223379935	0.0
35	0.0	0.0	0.0	10.329574478492606	0.0
36	0.0	0.0	0.0	10.560664735352512	0.0
37	0.0	0.0	0.0	10.795640782881675	0.0
38	0.0	0.0	0.0	11.026319603082483	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCCG	35	1.4468787E-7	44.0	26
CGACCGA	30	2.52877E-6	44.0	32
TCGATCT	20	7.8580435E-4	44.0	12
TATAGCG	125	0.0	44.0	1
CGTCCGT	20	7.8580435E-4	44.0	28
TTCGATC	30	2.52877E-6	44.0	31
ATTAGCG	170	0.0	42.70588	1
CGTTTTT	7685	0.0	42.568638	1
AGCGATA	270	0.0	42.37037	8
GAGCGAT	1460	0.0	42.041092	7
TTAGCGA	195	0.0	41.743587	2
TTCGAAG	145	0.0	40.965515	1
CGGTCTA	425	0.0	40.89412	31
CCGAGAC	55	7.8216544E-11	40.000004	5
GCGTAAG	245	0.0	39.510204	1
TCACGAC	435	0.0	39.448277	25
CGAACGG	95	0.0	39.36842	2
CGAGACC	225	0.0	39.11111	6
TGTTACG	85	0.0	38.82353	1
TGTAGCG	165	0.0	38.666668	1
>>END_MODULE