FastQCFastQC Report
Thu 26 May 2016
SRR1546756_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546756_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2591706
Sequences flagged as poor quality0
Sequence length50
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217140.8378265127294532No Hit
CGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133570.5153748148902692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA207.858313E-444.02
CGTTTTT318950.043.5102651
GAGCGAT16700.041.6287467
GTTTTTA141250.040.6669042
CGACGTC950.039.3684218
TCGTAGA1200.038.52
GAGACCG6750.037.8074047
CGAGGTT1750.037.7142876
AGCGATA4350.037.425298
AGACCGA6000.037.0333338
CGACGGT1800.036.66666828
CGACGAA301.3015464E-436.66666422
TACGAGA4750.036.1263164
GTTTTTT229700.036.0888142
GACCGAT13350.035.76039
CGGTCTA1850.035.6756831
TATTTTT162300.035.6364757
ACGTCGC1050.035.6190520
ACGGGAT8900.035.5955055
GCGCGAC2600.035.5384649
Produced by FastQC (version 0.11.5)